Succinyl-CoA synthetase
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Succinyl-CoA synthetase is a mitochondrial matrix enzyme and catalyst. Succinyl-CoA synthetase supports TCA, ketone and heme metabolism and is activated by mitochondrial phosphate. Succinyl-CoA synthetase distributes broadly across mammalian tissues with distinct substrate-related biochemical features. Succinyl-CoA synthetase gains structural stability after phosphorylation and relies on SUCLG1-encoded subunit for activity. Succinyl-CoA synthetase malfunction links to metabolic and neurological disorders. Succinyl-CoA synthetase serves as a research tool for mitochondrial hepatoencephalomyopathy.
For research use only. We do not sell to patients.
- CAS No.: 9080-33-5
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Succinyl-CoA synthetase exists as a tetramer, composed of two distinct subunits with molecular weights of 42500 and 34500 respectively; the smaller α subunit contains a histidine phosphorylation active site[1][2].
Succinyl-CoA synthetase gains improved structural stability and resistance to storage-related deactivation and trypsin-dependent proteolysis after phosphorylation-triggered conformational alterations[2].
Succinyl-CoA synthetase exhibits specificity for succinate at standard concentrations (malate, itaconate, and α-methylsuccinate can serve as weak or equivalently active alternative substrates), accepts dephospho-coenzyme A as a weak substitute for coenzyme A, and shows relaxed specificity for purine nucleotides. Its intrinsic purine nucleoside diphosphate kinase activity is regulated by succinyl-CoA and ATP[2].
Succinyl-CoA synthetase (1 mg protein/mL; 10 min) shows severely deficient activityin cultured human dermal fibroblasts with homozygous SUCLG c.40A > T mutation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
6.2.1.4
8 U/mg protein
One Unit is defined as the amount of enzyme required to release one μmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in glycylglycine buffer (34 mM), pH 8.4 at 25°C).
Chemical Information
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CAS No. 9080-33-5
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Succinyl-CoA synthetase]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Li X, et al. Identification of the kinetic mechanism of succinyl-CoA synthetase. Biosci Rep. 2013;33(1):145-163. Published 2013 Jan 18. [Content Brief]
[2]. Bridger WA. Succinyl-Coa Synthetase. In: Boyer PD, editor. The Enzymes. Vol 10; 1974. p.581-606.
[3]. Van Hove JL, et al. Succinyl-CoA ligase deficiency: a mitochondrial hepatoencephalomyopathy. Pediatr Res. 2010;68(2):159-164. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Succinyl-CoA synthetase Related Classifications
HY-E70121 Related Classifications
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