TVB-3166

Name: TVB-3166
Brand: MedChemExpress
SKU: HY-120394
Rating: 5.0 (12 reviews)

Cat. No.: HY-120394 Purity: 99.69%: Data Sheet
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TVB-3166 is an orally-available, reversible, and selective fatty acid synthase (FASN) inhibitor with IC₅₀s of 42 nM and 81 nM for biochemical FASN and cellular palmitate synthesis, respectively. TVB-3166 induces apoptosis, and inhibits in-vivo xenograft tumor growth.

For research use only. We do not sell to patients.

CAS No. : 1533438-83-3

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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Customer Review

Based on 12 publication(s) in Google Scholar

12 Publications Citing Use of MCE TVB-3166

ELISA

IHC

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

2D/3D Cell Culture and Differentiation

: TVB-3166 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 19;16(1):1762. [Abstract]; Inhibition of FASN by TVB-3166 reduced the protein levels of mutp53, but not wtp53, in different cells. Cells were treated with the small-molecule FASN inhibitor TVB-3166 (TVB) at the indicated concentrations for 24 h and subjected to Western blot analysis.

: TVB-3166 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 19;16(1):1762. [Abstract]; The FASN inhibitor TVB-3166 (TVB, 72 h) displayed a more pronounced inhibitory effect on AIG in cancer cells carrying mutp53 compared with cells with mutp53 KO (a) or knockdown (b). Left in a: Representative images. Scale bar: 200 μm.

: TVB-3166 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 19;16(1):1762. [Abstract]; TVB-3166 (TVB) displayed a more pronounced inhibitory effect on the growth of orthotopic tumors formed by SK-BR3 (R175H) cells than tumors formed by SK-BR3 with mutp53 KO. Mice were treated with TVB-3166 (60 mg/kg; i.g.) once every two days for 3 weeks when tumor sizes reached ~30 mm³.

: TVB-3166 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 19;16(1):1762. [Abstract]; TVB-3166 (40 μM) treatment displayed a more pronounced inhibitory effect on human colorectal tumor organoids expressing mutp53 compared with organoids expressing wtp53. Left panels: Representative images of different organoids expressing mutp53 or wtp53, which were treated with or without TVB-3166 for 48 h. Scale bar: 50 μm. Right panel: The relative viability of mutp53 and wtp53 organoids.

: TVB-3166 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Oct 24:192:118656. [Abstract]; Enzyme-linked immunosorbent assay (ELISA) was used to determine the FASN content of xenograft tumors in each group. The ELISA results showed that TVB-3166 (60 mg/kg; i.g.; 5 weeks) reduced the FASN gene of the tumor.

: TVB-3166 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2025 Oct 24:192:118656. [Abstract]; Statistical analysis was performed on the rate of KI67- and TUNEL-positive cells in each group of xenograft tumors by immunohistochemical staining (IHC). The corrected total cell fluorescence (CTCF) of BODIPY staining was used to quantitatively detect the lipid content of xenograft tumors in each group. The results showed that TVB-3166 (60 mg/kg; i.g.; 5 weeks) treatment can reduce lipid accumulation, inhibit cell proliferation and increase the apoptosis rate.

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Description

IC₅₀ & Target

IC50: 42 nM (FASN) and 81 nM (cellular palmitate synthesis)^[1]

In Vitro

ISX-9 promotes neurogenesis in vivo, enhancing the proliferation and differentiation of hippocampal subgranular zone (SGZ) neuroblasts, and the dendritic arborization of adult-generated dentate gyrus neurons. At 2.5-20 μM, ISX-9 has been shown to dose-dependently trigger neurogenesis and block gliogenesis in adult rat hippocampal stem cells through a calcium-activated signaling pathway dependent on myocyte-enhancer factor 2-dependent gene expression^[1].
Molecular exploration of ISX-9-induced regulation of neurogenesis (via FACS and microarray of SGZ stem and progenitor cells) suggested the involvement of the myocyte-enhancer family of proteins (Mef2)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	CALU-6 tumor cells
Concentration:	0.001, 0.01, 0.1, 1, 10 μM
Incubation Time:	24 hours
Result:	Caused cell death in CALU-6 non-small-cell lung tumor cells with a cellular IC₅₀ value of 0.10 μM.

Cell Viability Assay^[1]

Cell Line:	90 different tumor cell lines (such as CALU-6 NSCLC cell line, NCI-H1975 NSCLC cell line)
Concentration:	0.02 or 0.20 μM
Incubation Time:	7 days
Result:	Dose-dependent induction of cell death was observed in all tumor cell lines.

Western Blot Analysis^[1]

Cell Line:	COLO-205 and A549 cells
Concentration:	0.2 μM
Incubation Time:	48 hours
Result:	Inhibited β-catenin pathway signal transduction and transcriptional activity.

In Vivo

TVB-3166 (Oral gavage; 30-100 mg/kg/day) inhibits xenograft tumor growth^[1].
TVB-3166 (Oral gavage; 30-100 mg/kg/day) has the concentration is approximately 3-fold higher in plasma than tumor. The 100 and 30 mg/kg groups had plasma and tumor concentrations of 7 and 2.9 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB-c-nude mice^[1]
Dosage:	30, 60, or 100 mg/kg
Administration:	Oral gavage; once daily
Result:	Inhibited xenograft tumor growth.

Animal Model:	Female BALB-c-nude mice^[1]
Dosage:	30, 60, or 100 mg/kg (Pharmacokinetic Study)
Administration:	Oral gavage; once daily
Result:	The concentration was approximately 3-fold higher in plasma than tumor. The 100 and 30 mg/kg groups had plasma and tumor concentrations of 7 and 2.9 μM, respectively.

Molecular Weight

384.47

Formula

C₂₄H₂₄N₄O

CAS No.

1533438-83-3

Appearance

Solid

Color

White to off-white

SMILES

N#CC1=CC=C(C2CN(C(C3=CC(C4=NNC(C)=C4C)=C(C)C=C3C)=O)C2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (162.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6010 mL	13.0049 mL	26.0098 mL
5 mM	0.5202 mL	2.6010 mL	5.2020 mL
10 mM	0.2601 mL	1.3005 mL	2.6010 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.08 mg/mL (5.41 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (247 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Ventura R, et al. Inhibition of de novo Palmitate Synthesis by Fatty Acid Synthase Induces Apoptosis in Tumor Cells by Remodeling Cell Membranes, Inhibiting Signaling Pathways, and Reprogramming Gene Expression. EBioMedicine. 2015 Jul 2;2(8):808-24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6010 mL	13.0049 mL	26.0098 mL	65.0246 mL
	5 mM	0.5202 mL	2.6010 mL	5.2020 mL	13.0049 mL
	10 mM	0.2601 mL	1.3005 mL	2.6010 mL	6.5025 mL
	15 mM	0.1734 mL	0.8670 mL	1.7340 mL	4.3350 mL
	20 mM	0.1300 mL	0.6502 mL	1.3005 mL	3.2512 mL
	25 mM	0.1040 mL	0.5202 mL	1.0404 mL	2.6010 mL
	30 mM	0.0867 mL	0.4335 mL	0.8670 mL	2.1675 mL
	40 mM	0.0650 mL	0.3251 mL	0.6502 mL	1.6256 mL
	50 mM	0.0520 mL	0.2601 mL	0.5202 mL	1.3005 mL
	60 mM	0.0433 mL	0.2167 mL	0.4335 mL	1.0837 mL
	80 mM	0.0325 mL	0.1626 mL	0.3251 mL	0.8128 mL
	100 mM	0.0260 mL	0.1300 mL	0.2601 mL	0.6502 mL

TVB-3166 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TVB-31661533438-83-3TVB3166TVB 3166Fatty Acid Synthase (FASN)ApoptosisInhibitorinhibitorinhibit

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TVB-3166

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TVB-3166 Related Antibodies

12 Publications Citing Use of MCE TVB-3166

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Complete Stock Solution Preparation Table

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