Biotinyl tyramide
Based on 8 publication(s) in Google Scholar
Biotinyl tyramide is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols. Biotinyl tyramide can be used for the research of tyramide signal amplification.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 41994-02-9
- Formula: C18H25N3O3S
- Molecular Weight:363.47
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Biotinyl tyramide
More- Nature. 2025 May;641(8061):202-210. [Abstract]
- Nat Immunol. 2025 Mar;26(3):391-403. [Abstract]
- Nat Cell Biol. 2022 Apr;24(4):497-512. [Abstract]
- Nat Commun. 2023 Apr 25;14(1):2378. [Abstract]
- Genome Biol. 2022 Dec 15;23(1):259. [Abstract]
- Free Radic Biol Med. 2026 Mar 16:246:69-79. [Abstract]
- bioRxiv. 2026 May 21.
- Med Rev (2021). 2024 Jun 14;4(6):531-543. [Abstract]
Customer Validation & Images
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Biological Activity
Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
1. Solution preparation[7]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 27.5 mM (optimize according to the experiment).
Storage: Aliquot and store at -20°C or -80°C protected from light. Avoid repeated freeze-thaw cycles.
1.2 Preparation of working solution
Dilute with PBS or serum-free culture medium to 27.5 μM (optimize according to the experiment).
Note: The working solution should be freshly prepared, used immediately, and protected from light.
Biotinyl tyramide assay[7].
1. Wash HaCaT cells and HaCaT DUOX1 knockout fusion cells on coverslips with culture medium.
2. Add 1 μM Thapsigargin (HY-13433), and incubate at 37°C for 5 min in the presence or absence of 0.2 U/mL horseradish peroxidase and 27.5 μM Biotinyl tyramide.
3. On ice, wash the cells three times with PBS. The bound Biotinyl tyramide is visualized by incubation with fluorescent streptavidin (1:1000, prepared in PBS) at 4°C for 30 min.
4. Fix the cells with ice-cold 4% paraformaldehyde solution and stain the nuclei.
5. Observe using a fluorescence microscope.
Biotinyl tyramide (100 μM, 5 min) and Hemin (HY-19424) enhanced biotinylation in starved U2OS cells after confirming the G4-specific biotinylation activity of the RNA-Hemin complex in vitro[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 41994-02-9
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Appearance Solid
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Molecular Weight 363.47
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Formula C18H25N3O3S
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Color White to off-white
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SMILES
O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (8)
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Journal Impact Factor
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Most Recent
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Nature
2025 May;641(8061):202-210. PMID: 40140567
Biotinyl tyramide purchased from MedChemExpress. Usage Cited in: Nature. 2025 May;641(8061):202-210. [Abstract]
HEK293T cells expressing STING-APEX2 were first treated with 0.5 μM diBAZI, OVA peptide, or ABM5-OVA for 2 h, then with Biotinylated tyramide (500 μM; 30 min) and H2O2. Biotinylated proteins were pulled down by streptavidin magnetic beads and analyzed by western blot for TAP1 and proteasomes.
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Nat Immunol
The lactate receptor HCAR1 drives the recruitment of immunosuppressive PMN-MDSCs in colorectal cancer. [Abstract]2025 Mar;26(3):391-403. PMID: 39905201 -
Nat Cell Biol
2022 Apr;24(4):497-512. PMID: 35332264 -
Nat Commun
Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development. [Abstract]2023 Apr 25;14(1):2378. PMID: 37185772
Biotinyl tyramide purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Apr 25;14(1):2378. [Abstract]
Proximity labelling of peroxidase-based approach. Biotin-phenol (Biotinyl tyramide, 500 μM; 30 min) was oxidized into reactive phenoxyl radicals with hydrogen peroxide existence, thus enabling the proximal labelling. Biotinylated proteins from a proximity labeling experiment analyzed by biotin and streptavidin-HRP blot in E11.5 wild-type kidney rudiments. Clear changes in band pattern were observed for HRP-Flag-Ism1 treated samples compared with ligand-free samples. WCL, whole cell lysis.
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Genome Biol
G-quadruplexes sense natural porphyrin metabolites for regulation of gene transcription and chromatin landscapes. [Abstract]2022 Dec 15;23(1):259. PMID: 36522639 -
Free Radic Biol Med
Mitochondrial cysteinyl-tRNA synthetase 2 protects against excitotoxic retinal cell death via enhanced supersulfide production. [Abstract]2026 Mar 16:246:69-79. PMID: 41506554 -
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Med Rev (2021)
2024 Jun 14;4(6):531-543. PMID: 39664081
Biotinyl tyramide purchased from MedChemExpress. Usage Cited in: Med Rev (2021). 2024 Jun 14;4(6):531-543. [Abstract]
Biotinyl tyramide (500 μmol/L; 30 min). Immunofluorescence-validated APEX2-MST1 labeling specificity in KYSE150 cells, biotin red, MST1 green; right: expression of the APEX2-MST1 fusion protein was observed.
Biotinyl tyramide purchased from MedChemExpress. Usage Cited in: Med Rev (2021). 2024 Jun 14;4(6):531-543. [Abstract]
Biotinyl tyramide (500 μmol/L; 30 min) was used. Streptavidin-HRP Western blotting was performed to detect induced protein biotinylation in lysates from cells expressing APEX2-MST1.
Solvent & Solubility
DMSO : 100 mg/mL (275.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Evans M F, et al. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens [J]. BMC Clinical Pathology, 2003, 3: 1-17. [Content Brief]
[2]. Bobrow M N, et al. Catalyzed reporter deposition, a novel method of signal amplification application to immunoassays [J]. Journal of immunological methods, 1989, 125(1-2): 279-285. [Content Brief]
[3]. Kim S H, et al. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining [J]. Journal of Histochemistry & Cytochemistry, 2003, 51(1): 129-132. [Content Brief]
[4]. Hunyady B, et al. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining [J]. Journal of Histochemistry & Cytochemistry, 1996, 44(12): 1353-1362. [Content Brief]
[5]. Dráberová E, et al. Quantification of α-tubulin isotypes by sandwich ELISA with signal amplification through biotinyl-tyramide or immuno-PCR [J]. Journal of immunological methods, 2013, 395(1-2): 63-70. [Content Brief]
[6]. Kharel P, et al. Stress promotes RNA G-quadruplex folding in human cells [J]. Nature Communications, 2023, 14(1): 205. [Content Brief]
[7]. Pató A, et al. Hydrogen peroxide production by epidermal dual oxidase 1 regulates nociceptive sensory signals [J]. Redox Biology, 2023, 62: 102670. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.7513 mL | 13.7563 mL | 27.5126 mL | 68.7815 mL |
| 5 mM | 0.5503 mL | 2.7513 mL | 5.5025 mL | 13.7563 mL | |
| 10 mM | 0.2751 mL | 1.3756 mL | 2.7513 mL | 6.8781 mL | |
| 15 mM | 0.1834 mL | 0.9171 mL | 1.8342 mL | 4.5854 mL | |
| 20 mM | 0.1376 mL | 0.6878 mL | 1.3756 mL | 3.4391 mL | |
| 25 mM | 0.1101 mL | 0.5503 mL | 1.1005 mL | 2.7513 mL | |
| 30 mM | 0.0917 mL | 0.4585 mL | 0.9171 mL | 2.2927 mL | |
| 40 mM | 0.0688 mL | 0.3439 mL | 0.6878 mL | 1.7195 mL | |
| 50 mM | 0.0550 mL | 0.2751 mL | 0.5503 mL | 1.3756 mL | |
| 60 mM | 0.0459 mL | 0.2293 mL | 0.4585 mL | 1.1464 mL | |
| 80 mM | 0.0344 mL | 0.1720 mL | 0.3439 mL | 0.8598 mL | |
| 100 mM | 0.0275 mL | 0.1376 mL | 0.2751 mL | 0.6878 mL |
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