(Z)-Indirubin (Synonyms: (Z)-Couroupitine B; (Z)-Indigo red; (Z)-Indigopurpurin)

Cat. No.: HY-N0117A Purity: 99.95%: Data Sheet
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(Z)-Indirubin is an isomer of Indirubin (HY-N0117). Indirubin is a bis-indole alkaloid and has emarkable anticancer activity against chronic myelocytic leukemia.

For research use only. We do not sell to patients.

(Z)-Indirubin Chemical Structure

CAS No. : 906748-38-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
250 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

(Z)-Indirubin is an isomer of Indirubin (HY-N0117). Indirubin is a bis-indole alkaloid and has emarkable anticancer activity against chronic myelocytic leukemia^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	31 μM Compound: Indirubin	Inhibitory activity on the proliferation of Human lung carcinoma A549 cancer cells using MTT or sulforhodamine B assay Inhibitory activity on the proliferation of Human lung carcinoma A549 cancer cells using MTT or sulforhodamine B assay	[PMID: 15993584]
A549	IC₅₀	> 10 μM Compound: Indirubin	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
A549	IC₅₀	> 100 μM Compound: 4a	Antiproliferative activity against apoptosis resistant human A549 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis resistant human A549 cells after 72 hrs by MTT assay	[PMID: 26883150]
B16-F10	IC₅₀	0.2 μM Compound: 4a	Antiproliferative activity against apoptosis sensitive mouse B16F10 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 26883150]
Cancer cell lines	IC₅₀	> 100 μM Compound: Indirubin	Inhibitory activity on the proliferation of Human colon carcinoma Co12 cancer cells using MTT or sulforhodamine B assay Inhibitory activity on the proliferation of Human colon carcinoma Co12 cancer cells using MTT or sulforhodamine B assay	[PMID: 15993584]
DU-145	IC₅₀	6.8 μM Compound: Indirubin	Cytotoxicity against human DU145 cells after 24 hrs by MTT assay Cytotoxicity against human DU145 cells after 24 hrs by MTT assay	[PMID: 20627564]
HCT-116	IC₅₀	19.52 μM Compound: Indirubin	Cytotoxicity against human HCT-116 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 assay Cytotoxicity against human HCT-116 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 assay	[PMID: 37801824]
HL-60	IC₅₀	> 100 μM Compound: Indirubin	Inhibitory activity on the proliferation of Human myeloid leukemia HL-60 cancer cells using MTT or sulforhodamine B assay Inhibitory activity on the proliferation of Human myeloid leukemia HL-60 cancer cells using MTT or sulforhodamine B assay	[PMID: 15993584]
HT-1080	IC₅₀	42.2 μM Compound: Indirubin	Inhibitory activity on the proliferation of Human fibrosarcoma HT1080 cancer cells using MTT or sulforhodamine B assay Inhibitory activity on the proliferation of Human fibrosarcoma HT1080 cancer cells using MTT or sulforhodamine B assay	[PMID: 15993584]
HeLa	IC₅₀	40 μM Compound: 1	Cytotoxicity against human HeLa cells assessed as cell growth inhibition measured after 24 hrs by SRB assay Cytotoxicity against human HeLa cells assessed as cell growth inhibition measured after 24 hrs by SRB assay	[PMID: 34161865]
HeLa	IC₅₀	40 μM Compound: 58	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 24 hrs by SRB assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 24 hrs by SRB assay	[PMID: 36170798]
HeLa	IC₅₀	> 10 μM Compound: Indirubin	Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
HepG2	IC₅₀	> 10 μM Compound: Indirubin	Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
HepG2	IC₅₀	> 100 μM Compound: 1a	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by alamar blue assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by alamar blue assay	[PMID: 28743492]
Hs 683	IC₅₀	> 100 μM Compound: 4a	Antiproliferative activity against apoptosis sensitive human Hs683 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive human Hs683 cells after 72 hrs by MTT assay	[PMID: 26883150]
K562	IC₅₀	> 10 μM Compound: Indirubin	Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
K562	IC₅₀	> 100 μM Compound: 7	Antiproliferative activity against human K562 cells after 72 hrs by CCK8 assay Antiproliferative activity against human K562 cells after 72 hrs by CCK8 assay	[PMID: 29678463]
K562/VCR	IC₅₀	> 100 μM Compound: 7	Antiproliferative activity against human K562/VCR cells after 72 hrs by CCK8 assay Antiproliferative activity against human K562/VCR cells after 72 hrs by CCK8 assay	[PMID: 29678463]
LoVo	IC₅₀	2.2 μM Compound: Indirubin	Cytotoxicity against human LoVo cells after 24 hrs by MTT assay Cytotoxicity against human LoVo cells after 24 hrs by MTT assay	[PMID: 20627564]
LoVo	IC₅₀	> 100 μM Compound: 4a	Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay	[PMID: 26883150]
MCF7	IC₅₀	4 μM Compound: Indirubin	Antiproliferative activity against human MCF7 cells after 3 days by SRB assay Antiproliferative activity against human MCF7 cells after 3 days by SRB assay	[PMID: 24326279]
MCF7	IC₅₀	6.7 μM Compound: Indirubin	Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 20627564]
MCF7	IC₅₀	9.91 μM Compound: 1; 4a	Antiproliferative activity against human MCF7 cells after 3 days by SRB assay Antiproliferative activity against human MCF7 cells after 3 days by SRB assay	[PMID: 28557430]
MCF7	IC₅₀	> 10 μM Compound: Indirubin	Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
MCF7	IC₅₀	> 100 μM Compound: 4a	Antiproliferative activity against apoptosis sensitive human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive human MCF7 cells after 72 hrs by MTT assay	[PMID: 26883150]
MV4-11	IC₅₀	> 10 μM Compound: 1	Antiproliferative activity against wild type FLT3 expressing human MV411 cells after 72 hrs by WST8 assay Antiproliferative activity against wild type FLT3 expressing human MV411 cells after 72 hrs by WST8 assay	[PMID: 20153646]
Oocyte	IC₅₀	10 μM Compound: 1	Inhibition of starfish oocyte CDK1/Cyclin B using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by scintillation counter analysis Inhibition of starfish oocyte CDK1/Cyclin B using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by scintillation counter analysis	[PMID: 34161865]
Oocyte	IC₅₀	10 μM Compound: Indirubin	Inhibitory activity against cyclin dependent kinase 1-cyclinB from starfish (Marthasterias glacialis) oocytes Inhibitory activity against cyclin dependent kinase 1-cyclinB from starfish (Marthasterias glacialis) oocytes	[PMID: 14761195]
Oocyte	IC₅₀	6.5 μM Compound: 4	Inhibition of Cyclin-dependent kinase 1-cyclin B of M-phase Marthasterias glacialis oocytes Inhibition of Cyclin-dependent kinase 1-cyclin B of M-phase Marthasterias glacialis oocytes	[PMID: 15163202]
PC-3	IC₅₀	> 100 μM Compound: 4a	Antiproliferative activity against apoptosis sensitive human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive human PC3 cells after 72 hrs by MTT assay	[PMID: 26883150]
RAW264.7	IC₅₀	6.1 μM Compound: Indirubin	Cytotoxicity against mouse RAW264.7 cells after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells after 24 hrs by MTT assay	[PMID: 20627564]
RS4-11	IC₅₀	> 10 μM Compound: 1	Antiproliferative activity against human RS4:11 cells having FLT3-ITD mutation after 72 hrs by WST8 assay Antiproliferative activity against human RS4:11 cells having FLT3-ITD mutation after 72 hrs by WST8 assay	[PMID: 20153646]
SK-MEL-28	IC₅₀	> 100 μM Compound: 4a	Antiproliferative activity against apoptosis resistant human SK-MEL-28 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis resistant human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 26883150]
U-118-MG	IC₅₀	12.5 μM Compound: 1	Cytotoxicity against human U-118-MG cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against human U-118-MG cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 34161865]
U-373MG ATCC	IC₅₀	> 100 μM Compound: 4a	Antiproliferative activity against apoptosis resistant human U373 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis resistant human U373 cells after 72 hrs by MTT assay	[PMID: 26883150]
U-87MG ATCC	IC₅₀	12.5 μM Compound: 1	Cytotoxicity against human U87 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against human U87 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 34161865]

Molecular Weight

262.26

Formula

C₁₆H₁₀N₂O₂

CAS No.

906748-38-7

Appearance

Solid

Color

Dark purple to black

SMILES

O=C1/C(C2=CC=CC=C2N1)=C3NC4=CC=CC=C4C\3=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 4.17 mg/mL (15.90 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8130 mL	19.0650 mL	38.1301 mL
5 mM	0.7626 mL	3.8130 mL	7.6260 mL
10 mM	0.3813 mL	1.9065 mL	3.8130 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (257 KB) HNMR (266 KB) RP-HPLC (248 KB) MS (231 KB)

Handling Instructions (2659 KB)

References

[1]. Nguyen D T, et al. Synthesis of new indirubin derivatives and their in vitro anticancer activity[J]. Chemical Papers, 2019, 73: 1083-1092.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8130 mL	19.0650 mL	38.1301 mL	95.3252 mL
	5 mM	0.7626 mL	3.8130 mL	7.6260 mL	19.0650 mL
	10 mM	0.3813 mL	1.9065 mL	3.8130 mL	9.5325 mL
	15 mM	0.2542 mL	1.2710 mL	2.5420 mL	6.3550 mL

(Z)-Indirubin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(Z)-Indirubin906748-38-7(Z)-Couroupitine B (Z)-Indigo red (Z)-IndigopurpurinApoptosisAlkaloidisomerCouroupitine BleukemiaInhibitorinhibitorinhibit

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(Z)-Indirubin (Synonyms: (Z)-Couroupitine B; (Z)-Indigo red; (Z)-Indigopurpurin)

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Other Forms of (Z)-Indirubin:

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Complete Stock Solution Preparation Table

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