1. Apoptosis
  2. Apoptosis
  3. Indirubin

Indirubin  (Synonyms: Couroupitine B; Indigo red; Indigopurpurin)

Cat. No.: HY-N0117 Purity: 98.74%
COA Handling Instructions

Indirubin (Couroupitine B) is a bis-indole alkaloid and has emarkable anticancer activity against chronic myelocytic leukemia.

For research use only. We do not sell to patients.

Indirubin Chemical Structure

Indirubin Chemical Structure

CAS No. : 479-41-4

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 61 In-stock
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Solid
5 mg USD 55 In-stock
10 mg USD 77 In-stock
25 mg USD 132 In-stock
50 mg USD 220 In-stock
100 mg USD 385 In-stock
250 mg USD 715 In-stock
500 mg   Get quote  
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Indirubin:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Indirubin (Couroupitine B) is a bis-indole alkaloid and has emarkable anticancer activity against chronic myelocytic leukemia[1][2].

In Vitro

Indirubin (Couroupitine B) significantly inhibits Td-EC proliferation, migration, invasion, and angiogenesis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Human umbilical vein endothelial cells (HUVEC) line; tumor-derived endothelial cells (Td-EC).
Concentration: 5, 10μM
Incubation Time: 24, 48, 72 hours
Result: Inhibited Td-EC proliferation in a dose- and time-dependent manner.

Cell Invasion Assay[1]

Cell Line: Human umbilical vein endothelial cells (HUVEC) line; tumor-derived endothelial cells (Td-EC).
Concentration: 5μM
Incubation Time: overnight
Result: Inhibited Td-EC migration, invasion, and angiogenesis.
In Vivo

Indirubin (Couroupitine B) (12.5 mg/kg, 25 mg/kg; intraperitoneal injected; once a day for 14 days) may reduce the pathological alterations in a dose-dependent manner[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male mice (C57BL/6) [3]
Dosage: 12.5 mg/kg, 25 mg/kg
Administration: Indirubin (12.5 mg/kg, 25 mg/kg; intraperitoneal injected; once a day for 14 days)
Result: Attenuated alterations of lung structure induced by BLM.
Clinical Trial
Molecular Weight

262.26

Formula

C16H10N2O2

CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

O=C1NC2=C(C=CC=C2)/C1=C3NC4=C(C=CC=C4)C\3=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (19.07 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8130 mL 19.0650 mL 38.1301 mL
5 mM 0.7626 mL 3.8130 mL 7.6260 mL
10 mM 0.3813 mL 1.9065 mL 3.8130 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (3.81 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 98.74%

References
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Indirubin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Indirubin
Cat. No.:
HY-N0117
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