Indirubin (Synonyms: Couroupitine B; Indigo red; Indigopurpurin)

Name: Indirubin
Brand: MedChemExpress
SKU: HY-N0117
Rating: 5.0 (12 reviews)

Cat. No.: HY-N0117 Purity: 99.37%: Data Sheet
COA

SDS
Handling Instructions
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Indirubin (Couroupitine B) is a bis-indole alkaloid and has emarkable anticancer activity against chronic myelocytic leukemia.

For research use only. We do not sell to patients.

CAS No. : 479-41-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
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250 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Indirubin:

12 Publications Citing Use of MCE Indirubin

Cell Proliferation/Viability Assay

Cell Imaging/Staining

Apoptosis Analysis

In Vivo Efficacy Study

: Indirubin purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Feb 18:19:1083-1103. [Abstract]; Effect of Indirubin (0.01, 0.05, 0.1, 0.5, 1, 5, 10, 50 μM) on ALL cell proliferation. The CCK8 assay examined the inhibitory effect of Indirubin on the activity of ALL cell lines BALL-1 and Jurkat cells after treatment at different concentrations for 24, 48 and 72 hours.

: Indirubin purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Feb 18:19:1083-1103. [Abstract]; EdU assay to detect the effect of Indirubin (1, 5, 10 μM) on the proliferative capacity of ALL cells.

: Indirubin purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Feb 18:19:1083-1103. [Abstract]; Effect of Indirubin (1, 5, 10 μM) on apoptosis in ALL cells.

: Indirubin purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Feb 18:19:1083-1103. [Abstract]; Western blotting indicated that the expression of caspase 3 remains unchanged, whereas the expression of Bax and cleaved-caspase 3 increased, and the expression of Bcl-2 decreased treated with Indirubin (1, 5,10 μM).

: Indirubin purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2025 Feb 18:19:1083-1103. [Abstract]; Tumor weight after 3 weeks of Indirubin (25 mg/kg, i.g.) treatment.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Indirubin (Couroupitine B) is a bis-indole alkaloid and has emarkable anticancer activity against chronic myelocytic leukemia^[1]^[2].

In Vitro

Indirubin (Couroupitine B) significantly inhibits Td-EC proliferation, migration, invasion, and angiogenesis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Human umbilical vein endothelial cells (HUVEC) line; tumor-derived endothelial cells (Td-EC).
Concentration:	5, 10μM
Incubation Time:	24, 48, 72 hours
Result:	Inhibited Td-EC proliferation in a dose- and time-dependent manner.

Cell Invasion Assay^[1]

Cell Line:	Human umbilical vein endothelial cells (HUVEC) line; tumor-derived endothelial cells (Td-EC).
Concentration:	5μM
Incubation Time:	overnight
Result:	Inhibited Td-EC migration, invasion, and angiogenesis.

In Vivo

Indirubin (Couroupitine B) (12.5 mg/kg, 25 mg/kg; intraperitoneal injected; once a day for 14 days) may reduce the pathological alterations in a dose-dependent manner^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male mice (C57BL/6) ^[3]
Dosage:	12.5 mg/kg, 25 mg/kg
Administration:	Indirubin (12.5 mg/kg, 25 mg/kg; intraperitoneal injected; once a day for 14 days)
Result:	Attenuated alterations of lung structure induced by BLM.

Clinical Trial

Molecular Weight

262.26

Formula

C₁₆H₁₀N₂O₂

CAS No.

479-41-4

Appearance

Solid

Color

Brown to reddish brown

SMILES

O=C1NC2=C(C=CC=C2)/C1=C3NC4=C(C=CC=C4)C\3=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (19.07 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.8130 mL	19.0650 mL	38.1301 mL
5 mM	0.7626 mL	3.8130 mL	7.6260 mL
10 mM	0.3813 mL	1.9065 mL	3.8130 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (3.81 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.37%

Select Batch:

Data Sheet (284 KB) SDS (394 KB)

COA (257 KB) HNMR (258 KB) RP-HPLC (229 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Kim MH, et al. Indirubin, a purple 3,2- bisindole, inhibited allergic contact dermatitis via regulating T helper (Th)-mediated immune system in DNCB-induced model. J Ethnopharmacol. 2013 Jan 9;145(1):214-9. [Content Brief]

[2]. Hsieh WL, et al. Indirubin, an acting component of indigo naturalis, inhibits EGFR activation and EGF-induced CDC25B gene expression in epidermal keratinocytes. J Dermatol Sci. 2012 Aug;67(2):140-6. [Content Brief]

[3]. Zhang X, et al. Indirubin inhibits tumor growth by antitumor angiogenesis via blocking VEGFR2-mediated JAK/STAT3 signaling in endothelial cell. Int J Cancer. 2011 Nov 15;129(10):2502-11. [Content Brief]

[4]. Alex D, et al. Indirubin shows anti-angiogenic activity in an in vivo zebrafish model and an in vitro HUVEC model. J Ethnopharmacol. 2010 Sep 15;131(2):242-7. [Content Brief]

[5]. Li, Zhuohong, et al. Indirubin inhibits cell proliferation, migration, invasion and angiogenesis in tumor-derived endothelial cells. OncoTargets and therapy vol. 11 2937-2944. 18 May. 2018. [Content Brief]

[6]. Mohan, Lakshmi, et al. Indirubin, a bis-indole alkaloid binds to tubulin and exhibits antimitotic activity against HeLa cells in synergism with vinblastine. Biomed Pharmacother. 2018 Sep;105:506-517. [Content Brief]

[7]. Wang, Qi, et al. Indirubin alleviates bleomycin-induced pulmonary fibrosis in mice by suppressing fibroblast to myofibroblast differentiation. Biomedicine pharmacotherapy vol. 131 (2020): 110715. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8130 mL	19.0650 mL	38.1301 mL	95.3252 mL
	5 mM	0.7626 mL	3.8130 mL	7.6260 mL	19.0650 mL
	10 mM	0.3813 mL	1.9065 mL	3.8130 mL	9.5325 mL
	15 mM	0.2542 mL	1.2710 mL	2.5420 mL	6.3550 mL

Indirubin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indirubin479-41-4Couroupitine B Indigo red IndigopurpurinApoptosisIndirubin anticancerInhibitorinhibitorinhibit

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Indirubin (Synonyms: Couroupitine B; Indigo red; Indigopurpurin)

Customer Review

Other Forms of Indirubin:

Indirubin Related Antibodies

12 Publications Citing Use of MCE Indirubin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Indirubin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Indirubin Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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