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3-Methyltoxoflavin 

Cat. No.: HY-111117 Purity: 99.98%
Handling Instructions

3-Methyltoxoflavin is a potent Protein disulfide isomerase (PDI) inhibitor, with an IC50 of 170 nM.

For research use only. We do not sell to patients.

3-Methyltoxoflavin Chemical Structure

3-Methyltoxoflavin Chemical Structure

CAS No. : 32502-62-8

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 165 In-stock
Estimated Time of Arrival: December 31
5 mg USD 150 In-stock
Estimated Time of Arrival: December 31
10 mg USD 240 In-stock
Estimated Time of Arrival: December 31
50 mg USD 750 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1200 In-stock
Estimated Time of Arrival: December 31
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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

3-Methyltoxoflavin is a potent Protein disulfide isomerase (PDI) inhibitor, with an IC50 of 170 nM.

IC50 & Target

IC50: 170 nM (PDI)[1].

In Vitro

3-Methyltoxoflavin is a potent Protein disulfide isomerase (PDI) inhibitor, with an IC50 of 170 nM. 3-Methyltoxoflavin is toxic in a panel of human glioblastoma cell lines. From the screen, 3-Methyltoxoflavin emerges as the most cytotoxic inhibitor of PDI. Bromouridine labeling and sequencing (Bru-seq) of nascent RNA reveals that 3-Methyltoxoflavin induces Nrf2 antioxidant response, ER stress response, and autophagy. Specifically, 3-Methyltoxoflavin upregulates heme oxygenase 1 and SLC7A11 transcription and protein expression and represses PDI target genes such as TXNIP and EGR1. Interestingly, 3-Methyltoxoflavin-induced cell death does not proceed via apoptosis or necrosis, but by a mixture of autophagy and ferroptosis[1].

Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (723.97 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8265 mL 24.1324 mL 48.2649 mL
5 mM 0.9653 mL 4.8265 mL 9.6530 mL
10 mM 0.4826 mL 2.4132 mL 4.8265 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (12.07 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (12.07 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (12.07 mM); Clear solution

References
Cell Assay
[1]

The colon cancer cells are seeded in duplicate in 96-well plates at 7000-10000 cells per well. For the combination therapies, NAC is added to the well at the same time as 3-Methyltoxoflavin (35G8) (24 hours after plates are seeded), and Z-VAD-FMK and Necrostatin-1 are added to the well 1 hour prior to 3-Methyltoxoflavin addition. Cell growth inhibition is assessed by the cell viability rate. Cell viability is determined with the MTT assay. U87MG cells are seeded at 5000 cells per well in 96-well plates. Deferoxamine is added to cells in a five-point, three-fold dilution series from 400 μM. 3-Methyltoxoflavin is added immediately after in a five-point, three-fold dilution series from 100 μM. Cells are incubated with compounds for 12 hours[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

207.19

Formula

C₈H₉N₅O₂

CAS No.

32502-62-8

SMILES

CC1=NN(C)C(C2=N1)=NC(N(C)C2=O)=O

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
3-Methyltoxoflavin
Cat. No.:
HY-111117
Quantity:

3-Methyltoxoflavin

Cat. No.: HY-111117