3-Methyltoxoflavin
Based on 1 publication(s) in Google Scholar
3-Methyltoxoflavin is a potent Protein disulfide isomerase (PDI) inhibitor, with an IC50 of 170 nM.
For research use only. We do not sell to patients.
- Purity: 98.62%
- CAS No.: 32502-62-8
- Formula: C8H9N5O2
- Molecular Weight:207.19
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 3-Methyltoxoflavin
More
Biological Activity
IC50: 170 nM (PDI)[1].
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BHK-21 | CC50 |
6.2 μM
Compound: 33
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Cytotoxicity against hamster BHK-21 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity against hamster BHK-21 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
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[PMID: 36940609] |
| BT-474 | IC50 |
0.9 μM
Compound: 286a
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Antiproliferative activity against human BT-474 cells assessed as inhibition of cell growth
Antiproliferative activity against human BT-474 cells assessed as inhibition of cell growth
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[PMID: 28851503] |
| HepG2 | CC50 |
11 μM
Compound: 33
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Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
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[PMID: 36940609] |
| Huh-7 | CC50 |
>10 μM
Compound: GNF-Pf-2272
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NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
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[PMID: 18579783] |
| Huh-7 | CC50 |
1.1 μM
Compound: 33
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Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
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[PMID: 36940609] |
| Huh-7 | CC50 |
1.2 μM
Compound: 33
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Cytotoxicity against human Huh-7 cells infected with yellow fever virus assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human Huh-7 cells infected with yellow fever virus assessed as reduction in cell viability incubated for 48 hrs by MTT assay
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[PMID: 36940609] |
| Huh-7 | CC50 |
3.2 μM
Compound: 33
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Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
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[PMID: 36940609] |
| Vero 76 | CC50 |
1.7 μM
Compound: 33
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Cytotoxicity against African green monkey Vero 76 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against African green monkey Vero 76 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
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[PMID: 36940609] |
3-Methyltoxoflavin is a potent Protein disulfide isomerase (PDI) inhibitor, with an IC50 of 170 nM. 3-Methyltoxoflavin is toxic in a panel of human glioblastoma cell lines. From the screen, 3-Methyltoxoflavin emerges as the most cytotoxic inhibitor of PDI. Bromouridine labeling and sequencing (Bru-seq) of nascent RNA reveals that 3-Methyltoxoflavin induces Nrf2 antioxidant response, ER stress response, and autophagy. Specifically, 3-Methyltoxoflavin upregulates heme oxygenase 1 and SLC7A11 transcription and protein expression and represses PDI target genes such as TXNIP and EGR1. Interestingly, 3-Methyltoxoflavin-induced cell death does not proceed via apoptosis or necrosis, but by a mixture of autophagy and ferroptosis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 32502-62-8
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Appearance Solid
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Molecular Weight 207.19
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Formula C8H9N5O2
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Color Light yellow to yellow
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SMILES
CC1=NN(C)C(C2=N1)=NC(N(C)C2=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
DMSO : 50 mg/mL (241.32 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (24.13 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (24.13 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
The colon cancer cells are seeded in duplicate in 96-well plates at 7000-10000 cells per well. For the combination therapies, NAC is added to the well at the same time as 3-Methyltoxoflavin (35G8) (24 hours after plates are seeded), and Z-VAD-FMK and Necrostatin-1 are added to the well 1 hour prior to 3-Methyltoxoflavin addition. Cell growth inhibition is assessed by the cell viability rate. Cell viability is determined with the MTT assay. U87MG cells are seeded at 5000 cells per well in 96-well plates. Deferoxamine is added to cells in a five-point, three-fold dilution series from 400 μM. 3-Methyltoxoflavin is added immediately after in a five-point, three-fold dilution series from 100 μM. Cells are incubated with compounds for 12 hours[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.8265 mL | 24.1324 mL | 48.2649 mL | 120.6622 mL |
| 5 mM | 0.9653 mL | 4.8265 mL | 9.6530 mL | 24.1324 mL | |
| 10 mM | 0.4826 mL | 2.4132 mL | 4.8265 mL | 12.0662 mL | |
| 15 mM | 0.3218 mL | 1.6088 mL | 3.2177 mL | 8.0441 mL | |
| 20 mM | 0.2413 mL | 1.2066 mL | 2.4132 mL | 6.0331 mL | |
| 25 mM | 0.1931 mL | 0.9653 mL | 1.9306 mL | 4.8265 mL | |
| 30 mM | 0.1609 mL | 0.8044 mL | 1.6088 mL | 4.0221 mL | |
| 40 mM | 0.1207 mL | 0.6033 mL | 1.2066 mL | 3.0166 mL | |
| 50 mM | 0.0965 mL | 0.4826 mL | 0.9653 mL | 2.4132 mL | |
| 60 mM | 0.0804 mL | 0.4022 mL | 0.8044 mL | 2.0110 mL | |
| 80 mM | 0.0603 mL | 0.3017 mL | 0.6033 mL | 1.5083 mL | |
| 100 mM | 0.0483 mL | 0.2413 mL | 0.4826 mL | 1.2066 mL |
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