1. Membrane Transporter/Ion Channel
  2. P2X Receptor
  3. AF-353

AF-353 (Synonyms: Ro-4)

Cat. No.: HY-14483 Purity: 98.95%
Handling Instructions

AF-353 is a novel, potent and orally bioavailable P2X3/P2X2/3 receptor antagonist, inhibits human and rat P2X3 (pIC50= 8.0).

For research use only. We do not sell to patients.

AF-353 Chemical Structure

AF-353 Chemical Structure

CAS No. : 865305-30-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 132 In-stock
Estimated Time of Arrival: December 31
5 mg USD 120 In-stock
Estimated Time of Arrival: December 31
10 mg USD 192 In-stock
Estimated Time of Arrival: December 31
50 mg USD 780 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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  • Biological Activity

  • Technical Information

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Description

AF-353 is a novel, potent and orally bioavailable P2X3/P2X2/3 receptor antagonist, inhibits human and rat P2X3 (pIC50= 8.0). IC50 value: pIC50 = 8.0 (pIC50, for P2X3) Target: P2X3/P2X2/3 receptor in vitro: AF-353 is a highly potent inhibitor of α,β-meATP-evoked intracellular calcium flux in cell lines expressing recombinant rat and human P2X3 and human P2X2/3 channels. AF-353 also blocks human P2X2/3 channel function with marginally reduced potency (pIC50 = 7.3). [1] in vivo: SCI rats has significantly higher frequencies for field potentials and NVC than NL rats. Intravesical ATP increases field potential frequency in control but not SCI rats, while systemic AF-353 significantly reduces this parameter in both groups. AF-353 also reduces the inter-contractile interval in control but not in SCI rats; however, the frequency of NVC in SCI rats was significantly reduced.[2]

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 32 mg/mL (79.96 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4987 mL 12.4934 mL 24.9869 mL
5 mM 0.4997 mL 2.4987 mL 4.9974 mL
10 mM 0.2499 mL 1.2493 mL 2.4987 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

400.21

Formula

C₁₄H₁₇IN₄O₂

CAS No.

865305-30-2

SMILES

CC(C)C1=C(OC2=CN=C(N)N=C2N)C=C(I)C(OC)=C1

Shipping

Room temperature in continental US; may vary elsewhere

Purity: 98.95%

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Product Name:
AF-353
Cat. No.:
HY-14483
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AF-353

Cat. No.: HY-14483