CBB1007 trihydrochloride
Based on 2 publication(s) in Google Scholar
CBB1007 trihydrochloride is a reversible and selective LSD1 inhibitor with an IC50 of 5.27 µM for human LSD1. CBB1007 trihydrochloride significantly blocks the demethylase activity of LSD1 on H3K4Me2 and H3K4Me. CBB1007 trihydrochloride shows selectivity for LSD1 over LSD2 or JARID1A, and induces differentiation-related genes in pluripotent cells. CBB1007 trihydrochloride is studied in non-pluripotent cancer research, targeting teratocarcinoma, embryonic carcinoma, and seminoma.
For research use only. We do not sell to patients.
- Purity: 97.08%
- CAS No.: 2070015-03-9
- Formula: C27H37Cl3N8O4
- Molecular Weight:643.99
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) CBB1007 trihydrochloride
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Customer Validation & Images
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Biological Activity
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KDM1/LSD1 5.27 μM (IC50) |
CBB1007 (0-100 μM; 30 h; F9) trihydrochloride inhibits F9 cell growth[2].
CBB1007 (5-20 μM; 14 days; hESCs) trihydrochloride increases lipid droplet formation in hESCs during adipogenesis[1].
CBB1007 (5-20 μM; 14 days; hESCs) trihydrochloride reduces LSD1 and histone H3 levels while increasing H3K4me2 in human embryonic stem cells (hESCs)[1].
CBB1007 (5-20 μM; 14 days; hESCs) trihydrochloride upregulates adipocyte marker genes PPARγ-2 and C/EBPα in hESCs[1].
CBB1007 (0.5-20 μM; 24 h; F9) trihydrochloride activates the expression of CHRM4 and SCN3A genes[2].
CBB1007 (10 μM) trihydrochloride significantly blocks the demethylase activity of LSD1 on mono- and di-methylated H3K4, but not tri-methylated H3K4 or di-methylated H3K9[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:F9 cell
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Concentration:1 μM, 5 μM, 10 μM, 50 μM, 100 μM
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Incubation Time:30 h
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Result:Cell growth was significantly inhibited.
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Cell Line:hESCs
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Concentration:5 μM, 10 μM, 20 μM
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Incubation Time:14 days
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Result:Lipid droplet density increased, and droplets fused due to high density.
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Cell Line:hESCs
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Concentration:5 μM, 10 μM, 20 μM
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Incubation Time:14 days
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Result:LSD1 and histone H3 expression decreased, while H3K4me2 levels increased with higher doses.
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Cell Line:hESCs
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Concentration:5 μM, 10 μM, 20 μM
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Incubation Time:14 days
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Result:PPARγ-2 and C/EBPα expression increased with increasing doses.
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Cell Line:F9 cell
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Concentration:0.5 μM, 1 μM, 5 μM, 20 μM
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Incubation Time:24 h
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Result:The expression of CHRM4 and SCN3A genes were activated. And markedly induced the expression of genes for differentiation, such as FOXA2.
Chemical Information
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CAS No. 2070015-03-9
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Appearance Solid
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Molecular Weight 643.99
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Formula C27H37Cl3N8O4
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Color White to off-white
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SMILES
NC(N1CCN(CC1)CC2=CC(C(OC)=O)=CC(C(N3CCN(CC3)C(C4=CC=C(C=C4)C(N)=N)=O)=O)=C2)=N.[H]Cl.[H]Cl.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
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Journal Impact Factor
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Most Recent
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Clin Exp Med
Corin: a dual inhibitor for KDM1A/HDAC1, suppresses hepatocellular carcinoma by triggering cuproptosis. [Abstract]2025 Nov 25;26(1):33. PMID: 41286164 -
BMC Neurosci
Mechanism of LSD1 in oxygen-glucose deprivation/reoxygenation-induced pyroptosis of retinal ganglion cells via the miR-21-5p/NLRP12 axis. [Abstract]2022 Nov 10;23(1):63. PMID: 36357913
CBB1007 trihydrochloride purchased from MedChemExpress. Usage Cited in: BMC Neurosci. 2022 Nov 10;23(1):63. [Abstract]
CBB1007 (CBB) elevates H3K4me2 expression in RGC-5 cells, after which miR-21-5p expression levels are increased .
Solvent & Solubility
DMSO : 25 mg/mL (38.82 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (1.94 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (1.94 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Xiong Y, et al. Inhibition of Lysine-Specific Demethylase-1 (LSD1/KDM1A) Promotes the Adipogenic Differentiation of hESCs Through H3K4 Methylation. Stem Cell Rev Rep. 2016;12(3):298-304. [Content Brief]
[2]. Wang J, et al. Novel histone demethylase LSD1 inhibitors selectively target cancer cells with pluripotent stem cell properties. Cancer Res. 2011 Dec 1;71(23):7238-49. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5528 mL | 7.7641 mL | 15.5282 mL | 38.8205 mL |
| 5 mM | 0.3106 mL | 1.5528 mL | 3.1056 mL | 7.7641 mL | |
| 10 mM | 0.1553 mL | 0.7764 mL | 1.5528 mL | 3.8820 mL | |
| 15 mM | 0.1035 mL | 0.5176 mL | 1.0352 mL | 2.5880 mL | |
| 20 mM | 0.0776 mL | 0.3882 mL | 0.7764 mL | 1.9410 mL | |
| 25 mM | 0.0621 mL | 0.3106 mL | 0.6211 mL | 1.5528 mL | |
| 30 mM | 0.0518 mL | 0.2588 mL | 0.5176 mL | 1.2940 mL |
CBB1007 trihydrochloride Related Classifications
HY-15313C Related Classifications
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