2. GPCR/G Protein Neuronal Signaling
  3. CGRP Receptor
  4. HCGRP-(8-37)

HCGRP-(8-37)  (Synonyms: Human α-CGRP (8-37))

Cat. No.: HY-P1014 Purity: 99.51%
COA
SDS
Handling Instructions Technical Support

HCGRP-(8-37) (Human α-CGRP (8-37)) is a fragment of human calcitonin gene-related peptide (hCGRP) and an antagonist of the CGRP receptor, with an IC50 of 32.1 pM against the CGRP receptor. HCGRP-(8-37) blocks adenylate cyclase activation induced by CGRP receptor ligands and attenuates vascular responses triggered by CGRP. HCGRP-(8-37) reduces capsaicin-induced vasodilation in porcine nasal mucosa and superficial skin. HCGRP-(8-37) serves as a research tool to distinguish effects mediated by CGRP or calcitonin receptors, and to investigate CGRP-induced vascular effects.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

HCGRP-(8-37)

HCGRP-(8-37) Chemical Structure

CAS No. : 119911-68-1

Size Price Stock Quantity
500 μg In-stock
1 mg In-stock
5 mg In-stock
10 mg   Get quote  
50 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

HCGRP-(8-37) Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HCGRP-(8-37) (Human α-CGRP (8-37)) is a fragment of human calcitonin gene-related peptide (hCGRP) and an antagonist of the CGRP receptor, with an IC50 of 32.1 pM against the CGRP receptor. HCGRP-(8-37) blocks adenylate cyclase activation induced by CGRP receptor ligands and attenuates vascular responses triggered by CGRP. HCGRP-(8-37) reduces capsaicin-induced vasodilation in porcine nasal mucosa and superficial skin. HCGRP-(8-37) serves as a research tool to distinguish effects mediated by CGRP or calcitonin receptors, and to investigate CGRP-induced vascular effects[1][2].

IC50 & Target

CGRP receptor[1]
In Vitro

HCGRP-(8-37) (10 min at 37°C) acts as a CGRP receptor antagonist in rat liver plasma membranes. It inhibits CGRP- and calcitonin-induced adenylate cyclase activation in a dose-dependent manner, but does not affect basal, epinephrine- or glucagon-stimulated activities[1].
HCGRP-(8-37) (60 min at 37°C) binds to calcitonin receptors in LLC-PK1 porcine kidney cells in a dose-dependent manner[1].
HCGRP-(8-37) (15 min at 37°C) acts as a calcitonin receptor agonist in LLC-PK1 porcine kidney cells, which dose-dependently stimulates cAMP production without antagonizing calcitonin- or CGRP-induced cAMP production[1].
HCGRP-(8-37) potently inhibits CGRP-mediated adenylate cyclase activation in cell-free rat liver plasma membrane preparations[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HCGRP-(8-37) Related Antibodies
In Vivo

HCGRP-(8-37) (16 µM; local intra-arterial infusion; 20 minutes) significantly attenuates both CGRP- and capsaicin-mediated vasodilation in the pig nasal mucosa and reduces the integrated and duration-based capsaicin- and CGRP-mediated skin vasodilation responses, confirming it acts as a functional CGRP receptor antagonist in vivo[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Domestic pigs (either sex, 17-22 kg)[2]
Dosage: 16 µM
Administration: local intra-arterial infusion; 20 minutes
Result: Significantly attenuated capsaicin-evoked increases in nasal vascular conductance and decreases in nasal cavity volume.
Significantly reduced capsaicin-evoked superficial skin blood flow when calculated as area under the curve (AUC); capsaicin-induced skin peak blood flow remained unchanged (96% of control, not significant), but the duration of the skin response was significantly reduced to 69% of control .
Significantly reduced CGRP-evoked increases in nasal vascular conductance and decreases in nasal cavity volume.
Significantly attenuated CGRP-evoked superficial skin blood flow, with reductions seen in AUC, peak response, and response duration.
Molecular Weight

3125.59

Formula

C139H230N44O38
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asn-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe
Sequence Shortening

VTHRLAGLLSRSGGVVKNNFVPTNVGSKAF
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 20 mg/mL (6.40 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.3199 mL 1.5997 mL 3.1994 mL
5 mM 0.0640 mL 0.3199 mL 0.6399 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 20 mg/mL (6.40 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.51%

SDS (252 KB)

COA (241 KB) RP-HPLC (266 KB) MS (74 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 0.3199 mL 1.5997 mL 3.1994 mL 7.9985 mL
5 mM 0.0640 mL 0.3199 mL 0.6399 mL 1.5997 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

HCGRP-(8-37) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HCGRP-(8-37)119911-68-1Human α-CGRP (8-37)CGRP ReceptorCalcitonin gene-related peptide receptorCGRP receptorantagonistvasodilation
Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
HCGRP-(8-37)
Cat. No.:
HY-P1014
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.