1. Apoptosis
  2. Apoptosis
  3. INH6

INH6 

Cat. No.: HY-100541 Purity: 99.38%
Handling Instructions

INH6 is a potent Nek2/Hec1 inhibitor; inhibits the growth of HeLa cells with an IC50 of 2.4 μM.

For research use only. We do not sell to patients.

INH6 Chemical Structure

INH6 Chemical Structure

CAS No. : 1001753-24-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 50 In-stock
Estimated Time of Arrival: December 31
25 mg USD 110 In-stock
Estimated Time of Arrival: December 31
50 mg USD 180 In-stock
Estimated Time of Arrival: December 31
100 mg USD 340 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

INH6 is a potent Nek2/Hec1 inhibitor; inhibits the growth of HeLa cells with an IC50 of 2.4 μM.

IC50 & Target

IC50: 2.4 μM (HeLa), 1.7 μM (MB231), 2.1 μM (MB468), 2.5 μM (K562)[1]

In Vitro

Hec1 is an oncogene overly expressed in many human cancers. Small molecule INH (Inhibitor of Nek2/Hec1) targeting the Hec1 and its regulator, Nek2, in the mitotic pathway is identified to inactivate Hec1/Nek2 function mediated by protein degradation that subsequently leads to chromosome mis-segregation and cell death. INH6 treated cells exhibit increased mitotic population with multipolar spindle configurations. An increased rate of chromosome misalignment is detected upon treatment with INH6 of HeLa cells expressing the chromosome marker protein H2B-GFP. INH6 treated cells shows progressive morphological changes characteristic of dying cells (e.g., membrane bubbling), which is further confirmed by cell cycle profiling with FACS analysis. Approximately 20% of INH6 treated cells are apoptotic 72 hrs after treatment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

322.42

Formula

C₁₉H₁₈N₂OS

CAS No.

1001753-24-7

SMILES

O=C(NC1=NC(C2=C(C)C=C(C)C=C2C)=CS1)C3=CC=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (155.08 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1015 mL 15.5077 mL 31.0154 mL
5 mM 0.6203 mL 3.1015 mL 6.2031 mL
10 mM 0.3102 mL 1.5508 mL 3.1015 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (7.75 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

Standard XTT assays with a four-day drug treatment procedure were performed to measure the dose-dependent cytotoxicity of INH analogs in cultured cells. Triplicate sets were measured and compiled for final data presentation. Cells were plated on 96-well dishes one day before the drug treatment, followed by drug treatment (2.5 μM INH6) on day 2 and XTT assay on day 5 after drug addition. The absorption at 595 nm was measured with a plate reader and converted to cell survival percentages in comparison to mock treated groups[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Keywords:

INH6INH 6INH-6ApoptosisInhibitorinhibitorinhibit

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INH6
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