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ISO-1 (Synonyms: MIF Antagonist)

Cat. No.: HY-16692 Purity: 99.64%
Handling Instructions

ISO-1 is a macrophage migration inhibitory factor (MIF) antagonist with an IC50 of 7 μM.

For research use only. We do not sell to patients.

ISO-1 Chemical Structure

ISO-1 Chemical Structure

CAS No. : 478336-92-4

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 112 In-stock
Estimated Time of Arrival: December 31
5 mg USD 102 In-stock
Estimated Time of Arrival: December 31
10 mg USD 168 In-stock
Estimated Time of Arrival: December 31
50 mg USD 706 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    ISO-1 purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291.

    ISO-1 treatment partially recovers PCN033- or RS218-mediated downregulation of the tight junction proteins ZO-1 and occludin.

    ISO-1 purchased from MCE. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291.

    ISO-1 treatment partially recovers PCN033- or RS218-mediated downregulation of the tight junction proteins ZO-1 and occludin.
    • Biological Activity

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    • Customer Review

    Description

    ISO-1 is a macrophage migration inhibitory factor (MIF) antagonist with an IC50 of 7 μM.

    IC50 & Target

    IC50: 7μM (MIF)[1]

    In Vitro

    ISO-1 (0.1-20 μM; 16 hours) has a slight inhibitory effect on Cox-2 secretion without the addition of recombinant MIF[1].

    Western Blot Analysis[1]

    Cell Line: RAW 264.7 macrophage cells
    Concentration: 0.1μM,1μM,10μM,20μM
    Incubation Time: 16 hours
    Result: Suppressed Cox-2 secretion.
    In Vivo

    ISO-1 (injected intraperitoneally; 3.5-35 mg/kg; twice daily; 2 weeks) improves the survival rate from lethal endotoxemia and shows the anti-inflammatory effect[2].
    ISO-1 (intraperitoneal injection; 35 mg/kg; twice daily; 3 days) causes a significant reduction in average implant size and decreases Flk1 expression in implants[3].

    Animal Model: C57Bl/6 MIF+/+ and MIF–/– mice[2]
    Dosage: 3.5-35 mg/kg
    Administration: Injected intraperitoneally; 3.5-35 mg/kg; twice a day ; 2 weeks
    Result: Was protective against lethal sepsis.
    Animal Model: Female C57BL/6-Tg(ACTB-EGFP)1Osb/J mice[3]
    Dosage: 35 mg/kg
    Administration: Intraperitoneal injection; 35 mg/kg; twice daily; 3 days
    Result: Reduced average endometriotic implant size.
    Molecular Weight

    235.24

    Formula

    C₁₂H₁₃NO₄

    CAS No.

    478336-92-4

    SMILES

    O=C(OC)CC1ON=C(C(C=C2)=CC=C2O)C1

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (212.55 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.2510 mL 21.2549 mL 42.5098 mL
    5 mM 0.8502 mL 4.2510 mL 8.5020 mL
    10 mM 0.4251 mL 2.1255 mL 4.2510 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References

    Purity: 99.64%

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    Inquiry Information

    Product Name:
    ISO-1
    Cat. No.:
    HY-16692
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