1. Immunology/Inflammation
  2. NO Synthase
  3. L-Canavanine sulfate

L-Canavanine sulfate 

Cat. No.: HY-B1581A Purity: >98.0%
Handling Instructions

L-Canavanine sulfate is a selective inhibitor of inducible NO synthase.

For research use only. We do not sell to patients.

L-Canavanine sulfate Chemical Structure

L-Canavanine sulfate Chemical Structure

CAS No. : 2219-31-0

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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

Publications Citing Use of MCE L-Canavanine sulfate

    L-Canavanine sulfate purchased from MCE. Usage Cited in: Oncol Rep. 2018 Jun;39(6):2595-2603.

    The effect of CuD (2 μM), SC79 (4 μg/mL), and L‑canavanine (1 mM) on cell apoptosis in the AGS cell line.
    • Biological Activity

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    Description

    L-Canavanine sulfate is a selective inhibitor of inducible NO synthase.

    IC50 & Target

    NO synthase[1]

    In Vitro

    L-Canavanine sulfate (L-CAV) causes only a limited degree of cytotoxicity in HeLa, Hep G2, and SK-HEP-1 cells when given alone in arginine-rich media with IC50 values ranging from 5 to 10 mM. In HaCaT keratinocyte cell line, IC50 of L-Canavanine sulfate exceeds the concentration of 10 mM, indicating low cytotoxicity in normal cells in vitro. In arginine-free media, IC50 of L-Canavanine sulfate in HeLa, Hep G2, and SK-HEP-1 cells are 0.21±0.04; 0.64±0.16; and 1.18±0.14 mM, respectively. L-Canavanine sulfate, which is hardly toxic alone, potentiates the cytotoxicity of vinblastine (VIN) and paclitaxel (PTX) in HeLa and hepatocellular carcinoma cells[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Administration of L-Canavanine sulfate (100 mg/kg) produces a moderate increase in mean arterial pressure of 20 mm Hg, returns blood pressure to near basal levels and completely attenuates the lipopolysaccharide-induced hypotension. All, but one, endotoxaemic rats dosed with L-Canavanine sulfate (100 mg/kg) survive for 6 h post lipopolysaccharide, after which time the experiment is terminated (n=7)[1]. L-canavanine inhibits DNA synthesis by Li 210 cells in vivo and significantly increases the lifespan of animals bearing the Li 210 leukemia. An optimal dose of 18 g/kg produces a peak increase in lifespan of 44%. The therapeutic dose range is narrow, and a dose of 24 g/kg causes death due to drug toxicity[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    274.25

    Formula

    C₅H₁₄N₄O₇S

    CAS No.

    2219-31-0

    SMILES

    N[[email protected]@H](CCONC(N)=N)C(O)=O.O=S(O)(O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (364.63 mM; Need ultrasonic and warming)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.6463 mL 18.2315 mL 36.4631 mL
    5 mM 0.7293 mL 3.6463 mL 7.2926 mL
    10 mM 0.3646 mL 1.8232 mL 3.6463 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [2]

    A dose-dependent cytotoxicity is examined using the MTT assay. Into each well of 96-well plates, 2×104 of cells are seeded, and after 24 h incubation, cells are incubated with test compounds (including L-Canavanine sulfate). After 24 h, 0.5 mg/mL of MTT is added to each well of HeLa, Caco-2, MIA PaCa-2, BxPC-3 and SK-HEP-1 cells, while MTT is added to Hep G2 after 48 h incubation with test compounds (including L-Canavanine sulfate). The cells are then incubated for 3 h so that the viable cells could produce formazan crystals; they are then dissolved in 100 μL DMSO. After incubation for 10 min in a shaker, the absorption of the formazan is measured at 570 nm using a reader[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    Male rats weighing 295 to 305 g are used. After a period of stabilization, 6 mg lipopolysaccharide/kg is administered intravenously in 0.3 mL over 2 min, and cardiovascular parameters are monitored over 5 to 6 h. L-Canavanine sulfate is administered intravenously in a 0.2 mL volume bolus injection at 100 mg/kg[1].

    Mice: L-Canavanine is dissolved in phosphate-buffered saline[1]. L-Canavanine (100 mg/mb) is infused at a rate of 0.1 mL/hr through a catheter implanted S.C. over the back. Groups of 5 mice are treated at each dose beveltogether with 6 to 8 control animals and are inspected twice per day for median time of death[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Keywords:

    L-CanavanineNO SynthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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    Cat. No.:
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