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LOM612 

Cat. No.: HY-101035
Handling Instructions

LOM612 is a potent activator of FOXO nuclear translocation, with an EC50 value of 1.5 μM in cells.

For research use only. We do not sell to patients.

LOM612 Chemical Structure

LOM612 Chemical Structure

CAS No. : 2173232-79-4

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

LOM612 is a potent activator of FOXO nuclear translocation, with an EC50 value of 1.5 μM in cells.

IC50 & Target

EC50: 1.5 μM (FOXO)[1]

In Vitro

LOM612 potently activates nuclear translocation of FOXO with an EC50 value of 1.5 μM, and this effect is independent of CRM-1. LOM612 effectively induces translocation of endogenous FOXO3a and FOXO1, and increases the expression of the FOXO target genes p27 and FasL. LOM612 shows no effect on the nuclear export of endogenous NFKB2 transcription factor in U2OS cells. LOM612 is cytotoxic to HepG2 cells, with an IC50 value of 0.64 μM, and does not sensitize non-cancer THLE2 cells (IC50, 2.76 μM)[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8715 mL 19.3573 mL 38.7147 mL
5 mM 0.7743 mL 3.8715 mL 7.7429 mL
10 mM 0.3871 mL 1.9357 mL 3.8715 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Cells are seeded at a concentration of 1× 104 cells/well in 200 μL culture medium and incubated at 37°C in 5% CO2. After 24 hours, when the monolayer formed, the medium is replaced with a final volume of 200 μL of new medium with tested compounds (LOM612, etc.) or controls are added to the plates. Cells are treated with eight 2-fold serial dilutions of each compound spanning concentrations from 50 μM to 0.39 μM in 1% DMSO final. Controls are on the first and the last columns of the plates. On the first column, methyl methanesulfonate (MMS) acts as a positive control and DMSO as a negative control. On the last column there are four points of rotenone and doxorubicin with an initial concentration of 10 mM and dilution ½. When compounds (LOM612, etc.) and controls are added, plates are incubated at 37°C in 5% CO2 incubator for 72 hours. After this time, MTT solution is prepared at 5 mg/mL in PBS 1X and then diluted at 0.5 mg/mL in MEM without phenol red. The sample solution in wells is flicked off and 100 μL of MTT dye is added to each well. The plates are gently shaken and incubated for 3 hours at 37°C in 5% CO2 incubator. The supernatant is removed and 100 μL of DMSO 100% is added. The plates are gently shaken to solubilize the formed formazan. The absorbance is measured at a wavelength of 570 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

258.30

Formula

C₁₃H₁₀N₂O₂S

CAS No.

2173232-79-4

SMILES

O=C1C2=C(SN=C2N(C)C)C(C3=CC=CC=C31)=O

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
LOM612
Cat. No.:
HY-101035
Quantity:

LOM612

Cat. No.: HY-101035