2. Stem Cell/Wnt TGF-beta/Smad
  3. TGF-beta/Smad
  4. Menaquinone-7

Menaquinone-7  (Synonyms: Vitamin K2-7; Vitamin K2(35); Vitamin MK-7)

Cat. No.: HY-112499 Purity: 98.51%
COA
SDS
Handling Instructions Technical Support

Menaquinone-7 (Vitamin K2-7), belongs to a class of K2-vitamin homologs (orally active), is originally discovered as the anti-hemorrhagic factors. Menaquinone-7 inhibits osteoclast bone resorption in vitro and stimulates bone formation in femoral tissue of aged female rats. Menaquinone-7 has a well-researched potential in the prevention of aging-induced bone degeneration. Menaquinone-7 is also a pharmacological option for activating Gla matrix protein and intervening in the progression of calcific aortic stenosis (CAVS).

For research use only. We do not sell to patients.

CAS No. : 2124-57-4

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Menaquinone-7:

Menaquinone-7 Related Antibodies

Top Publications Citing Use of Products

    Menaquinone-7 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 May 16:175:116697.  [Abstract]

    The CCK8 method was used to screen for the working concentration of in vitro Menaquinone-7 (VK).

    Menaquinone-7 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 May 16:175:116697.  [Abstract]

    Menaquinone-7 (VK: 0.5/1 μM). Statistical data of apoptosis detected by flow cytometry.

    Menaquinone-7 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2024 May 16:175:116697.  [Abstract]

    Menaquinone-7 (VK: 0.5/1 μM). WB results for ACAN, COL2A1, SOX9, MMP3, MMP13 and Tubulin.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Menaquinone-7 (Vitamin K2-7), belongs to a class of K2-vitamin homologs (orally active), is originally discovered as the anti-hemorrhagic factors. Menaquinone-7 inhibits osteoclast bone resorption in vitro and stimulates bone formation in femoral tissue of aged female rats. Menaquinone-7 has a well-researched potential in the prevention of aging-induced bone degeneration. Menaquinone-7 is also a pharmacological option for activating Gla matrix protein and intervening in the progression of calcific aortic stenosis (CAVS)[1][2][3][4][5].

    IC50 & Target

    Smad1

     

    In Vitro

    Menaquinone-7 (0.01-10 μM; 7 days) inhibits osteoclast-like cell formation in marrow cultures[1].
    Menaquinone-7 (10 μM) promotes the expression of tenascin C and increases phosphorylated Smad1 level in MC3T3E1 cells[2].
    Menaquinone-7 (1, 10 μM) significantly enhances the anabolic effect on the femoral calcium content in the presence of phytoestrogen genistein (1, 10 μM)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Menaquinone-7 Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Marrow cells (from 3-week-old male wistar rats; PTH/PGE2 stimulated)
    Concentration: 0.01-10 µM
    Incubation Time: 7 days
    Result: Significantly decreased the number of TRACP-positive MNCs stimulated by PTH or PGE2.
    In Vivo

    Menaquinone-7 (18.1 mg/100 g diet; in animal feedings; 24 days) shows a preventive effect on bone loss in ovariectomized rats[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female Wistar rats (5-week-old; ovariectomized (OVX) rats model)[5].
    Dosage: 18.1 mg/100 g diet
    Administration: In animal feedings; 24 days
    Result: Had a preventive effect on OVX-induced bone loss.
    Clinical Trial
    Molecular Weight

    649.00

    Formula

    C46H64O2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C1C(C/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)/CC/C=C(C)\C)=C(C)C(C2=C1C=CC=C2)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    Ethanol : 10 mg/mL (15.41 mM; Need ultrasonic)

    DMSO : 5 mg/mL (7.70 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5408 mL 7.7042 mL 15.4083 mL
    5 mM 0.3082 mL 1.5408 mL 3.0817 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 0.54 mg/mL (0.83 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 0.54 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.4 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 0.54 mg/mL (0.83 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.54 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.4 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.51%

    SDS (393 KB)

    COA (239 KB) HNMR (301 KB) RP-HPLC (243 KB) MS (85 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / Ethanol 1 mM 1.5408 mL 7.7042 mL 15.4083 mL 38.5208 mL
    5 mM 0.3082 mL 1.5408 mL 3.0817 mL 7.7042 mL
    Ethanol 10 mM 0.1541 mL 0.7704 mL 1.5408 mL 3.8521 mL
    15 mM 0.1027 mL 0.5136 mL 1.0272 mL 2.5681 mL
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    Menaquinone-7 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Menaquinone-72124-57-4Vitamin K2-7 Vitamin K2(35) Vitamin MK-7Menaquinone7Menaquinone 7Vitamin MK7Vitamin MK 7Vitamin MK-7TGF-beta/SmadTransforming growth factor betaCAVSmarrow cellMC3T3E1 cellsosteoporosisanti-hemorrhagicInhibitorinhibitorinhibit

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