NSC348884

Name: NSC348884
Brand: MedChemExpress
SKU: HY-13915
Rating: 5.0 (3 reviews)

Cat. No.: HY-13915 Purity: 99.40%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

NSC348884 is a nucleophosmin (NPM) inhibitor, it disrupts oligomer formation and induces apoptosis, inhibits cell proliferation with IC₅₀s of 1.7-4.0 μM in distinct cancer cell lines. NSC348884 can be used for the research of cancer.

For research use only. We do not sell to patients.

CAS No. : 81624-55-7

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE NSC348884

Cell Proliferation/Viability Assay

RT-PCR

: NSC348884 purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2021 May 25:12:679341. [Abstract]; NPM1 inhibitor NSC348884 (0.1, 1 μM) and DMSO-treated cells were infected with PCV3 for 48, 72, and 96 h. Western blot was used to examine the expression levels of Cap, NPM1, and β-actin

: NSC348884 purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2021 May 25:12:679341. [Abstract]; NPM1 expression in NSC348884 (1 μM)- and DMSO-treated cells analyzed by immunofluorescence.

: NSC348884 purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2021 May 25:12:679341. [Abstract]; Viability of PK15 cells treated with NSC348884 (0.1, 1 μM) and DMSO analyzed using a CCK-8 assay.

: NSC348884 purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2021 May 25:12:679341. [Abstract]; qPCR was exploited to determine the expression level of Cap gene treated with NSC348884.

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References
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Description

IC₅₀ & Target

IC50: 4 μM (LNCaP cells), 1.7 μM (Granta cells)^[1]

In Vitro

NSC348884 (0-6 μM; 24 h) binds with NPM and disrupts NPM oligomer formation^[1].
NSC348884 (0.01-100 μM; 4 d) shows cytotoxicity to cancer cells^[1].
NSC348884 (1 μM; 4 d) shows cytotoxicity to mantle cell lymphoma with 0.1 nM doxorubicin^[1].
NSC348884 (1-5 μM; 24 h) induces cell apoptosis^[1].
NSC348884 (0-5 μM; 0-24 h) upregulates the concentration of p53^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	LNCaP and Granta cell lines
Concentration:	0.01-100 μM
Incubation Time:	4 days
Result:	Inhibited LNCaP cells and Granta cell lines with IC₅₀s of 4 and 1.7 μM, respectively.

Apoptosis Analysis^[1]

Cell Line:	LNCaP cell line
Concentration:	1-5 μM
Incubation Time:	24 h
Result:	Induced apoptosis in LNCaP cell line.

Western Blot Analysis^[1]

Cell Line:	LNCaP cell line
Concentration:	0-5 μM
Incubation Time:	0-24 h
Result:	Dose-dependently increased both p53 protein level and phosphorylation on Ser15, and increased the concentration of p21.

Western Blot Analysis^[2]

Cell Line:	SKOV3, ES2 and MOSEC/Luc cell lines
Concentration:	0, 2.5 and 5 μM
Incubation Time:	48 h
Result:	Increased PD-L1 expression in ovarian cancer cells.

In Vivo

NSC348884 (50 μg; i.p. once) reduces tumor growth^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57Bl/6J mice with MOSEC/Luc cells injection^[2]
Dosage:	50 μg
Administration:	Intraperitoneal injection; 50 μg once
Result:	Reduced tumorigenesis, but not completely inhibited.

Molecular Weight

636.79

Formula

C₃₈H₄₀N₁₀

CAS No.

81624-55-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

CC1=CC=C2N=C(CN(CC3=NC4=CC=C(C)C=C4N3)CCN(CC5=NC6=CC=C(C)C=C6N5)CC7=NC8=CC=C(C)C=C8N7)NC2=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 34 mg/mL (53.39 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5704 mL	7.8519 mL	15.7038 mL
5 mM	0.3141 mL	1.5704 mL	3.1408 mL
10 mM	0.1570 mL	0.7852 mL	1.5704 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.93 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.93 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.40%

Select Batch:

Data Sheet (280 KB) SDS (392 KB)

COA (252 KB) HNMR (274 KB) LCMS (108 KB)

Handling Instructions (2659 KB)

References

[1]. Qi W, et al. NSC348884, a nucleophosmin inhibitor disrupts oligomer formation and induces apoptosis in human cancercells. Oncogene. 2008 Jul 10;27(30):4210-20. [Content Brief]

[2]. Lin Y, et al. Inhibition of nucleophosmin/B23 sensitizes ovarian cancer cells to antiprogrammed cell death protein-1 ligand therapy. 2022.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.5704 mL	7.8519 mL	15.7038 mL	39.2594 mL
	5 mM	0.3141 mL	1.5704 mL	3.1408 mL	7.8519 mL
	10 mM	0.1570 mL	0.7852 mL	1.5704 mL	3.9259 mL
	15 mM	0.1047 mL	0.5235 mL	1.0469 mL	2.6173 mL
	20 mM	0.0785 mL	0.3926 mL	0.7852 mL	1.9630 mL
	25 mM	0.0628 mL	0.3141 mL	0.6282 mL	1.5704 mL
	30 mM	0.0523 mL	0.2617 mL	0.5235 mL	1.3086 mL
	40 mM	0.0393 mL	0.1963 mL	0.3926 mL	0.9815 mL
	50 mM	0.0314 mL	0.1570 mL	0.3141 mL	0.7852 mL

NSC348884 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NSC34888481624-55-7NSC 348884NSC-348884Apoptosissmall molecularp53apoptosisdoxorubicincancerLNCaPlymphomaSKOV3ES2MOSEC/Luc cellInhibitorinhibitorinhibit

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NSC348884

Customer Review

NSC348884 Related Antibodies

3 Publications Citing Use of MCE NSC348884

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Complete Stock Solution Preparation Table

NSC348884 Related Classifications

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