2. Apoptosis
  3. Apoptosis
  4. NSC348884

NSC348884 is a nucleophosmin (NPM) inhibitor, it disrupts oligomer formation and induces apoptosis, inhibits cell proliferation with IC50s of 1.7-4.0 μM in distinct cancer cell lines. NSC348884 can be used for the research of cancer.

For research use only. We do not sell to patients.

NSC348884 Chemical Structure

NSC348884 Chemical Structure

CAS No. : 81624-55-7

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10 mM * 1 mL in DMSO
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Based on 3 publication(s) in Google Scholar

NSC348884 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

NSC348884 is a nucleophosmin (NPM) inhibitor, it disrupts oligomer formation and induces apoptosis, inhibits cell proliferation with IC50s of 1.7-4.0 μM in distinct cancer cell lines. NSC348884 can be used for the research of cancer[1].

IC50 & Target

IC50: 4 μM (LNCaP cells), 1.7 μM (Granta cells)[1]
In Vitro

NSC348884 (0-6 μM; 24 h) binds with NPM and disrupts NPM oligomer formation[1].
NSC348884 (0.01-100 μM; 4 d) shows cytotoxicity to cancer cells[1].
NSC348884 (1 μM; 4 d) shows cytotoxicity to mantle cell lymphoma with 0.1 nM doxorubicin[1].
NSC348884 (1-5 μM; 24 h) induces cell apoptosis[1].
NSC348884 (0-5 μM; 0-24 h) upregulates the concentration of p53[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NSC348884 Related Antibodies

Cell Viability Assay[1]

Cell Line: LNCaP and Granta cell lines
Concentration: 0.01-100 μM
Incubation Time: 4 days
Result: Inhibited LNCaP cells and Granta cell lines with IC50s of 4 and 1.7 μM, respectively.

Apoptosis Analysis[1]

Cell Line: LNCaP cell line
Concentration: 1-5 μM
Incubation Time: 24 h
Result: Induced apoptosis in LNCaP cell line.

Western Blot Analysis[1]

Cell Line: LNCaP cell line
Concentration: 0-5 μM
Incubation Time: 0-24 h
Result: Dose-dependently increased both p53 protein level and phosphorylation on Ser15, and increased the concentration of p21.

Western Blot Analysis[2]

Cell Line: SKOV3, ES2 and MOSEC/Luc cell lines
Concentration: 0, 2.5 and 5 μM
Incubation Time: 48 h
Result: Increased PD-L1 expression in ovarian cancer cells.
In Vivo

NSC348884 (50 μg; i.p. once) reduces tumor growth[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57Bl/6J mice with MOSEC/Luc cells injection[2]
Dosage: 50 μg
Administration: Intraperitoneal injection; 50 μg once
Result: Reduced tumorigenesis, but not completely inhibited.
Molecular Weight

636.79

Formula

C38H40N10
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

CC1=CC=C2N=C(CN(CC3=NC4=CC=C(C)C=C4N3)CCN(CC5=NC6=CC=C(C)C=C6N5)CC7=NC8=CC=C(C)C=C8N7)NC2=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 34 mg/mL (53.39 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5704 mL 7.8519 mL 15.7038 mL
5 mM 0.3141 mL 1.5704 mL 3.1408 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (3.93 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (3.93 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5704 mL 7.8519 mL 15.7038 mL 39.2594 mL
5 mM 0.3141 mL 1.5704 mL 3.1408 mL 7.8519 mL
10 mM 0.1570 mL 0.7852 mL 1.5704 mL 3.9259 mL
15 mM 0.1047 mL 0.5235 mL 1.0469 mL 2.6173 mL
20 mM 0.0785 mL 0.3926 mL 0.7852 mL 1.9630 mL
25 mM 0.0628 mL 0.3141 mL 0.6282 mL 1.5704 mL
30 mM 0.0523 mL 0.2617 mL 0.5235 mL 1.3086 mL
40 mM 0.0393 mL 0.1963 mL 0.3926 mL 0.9815 mL
50 mM 0.0314 mL 0.1570 mL 0.3141 mL 0.7852 mL
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NSC348884 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NSC34888481624-55-7NSC 348884NSC-348884Apoptosissmall molecularp53apoptosisdoxorubicincancerLNCaPlymphomaSKOV3ES2MOSEC/Luc cellInhibitorinhibitorinhibit

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