Penicillamine (Synonyms: D-(-)-Penicillamine)

Name: Penicillamine
Brand: MedChemExpress
SKU: HY-B0300
Rating: 5.0 (3 reviews)

Cat. No.: HY-B0300 Purity: 98.0%: Data Sheet
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Penicillamine (D-(-)-Penicillamine) is a penicillin metabolic degradation product, can be used as a heavy metal chelator. Penicillamine increases free copper and increases oxidative stress. Penicillamine has effect of seizures through nitric oxide/NMDA pathways. Penicillamine is a potential immune modulator. Penicillamine can be used for the research of Wilson disease, rheumatoid arthritis, and cystinuria.

For research use only. We do not sell to patients.

CAS No. : 52-67-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Based on 3 publication(s) in Google Scholar

Other Forms of Penicillamine:

3 Publications Citing Use of MCE Penicillamine

Microbiological Assay

ELISA

: Penicillamine purchased from MedChemExpress. Usage Cited in: J Colloid Interface Sci. 2025 Jun 12;699(Pt 1):138178. [Abstract]; Effects of Penicillamine (0-400 μM) on MRSA cell viability after CuSA-COF treatment.

: Penicillamine purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2021 Jun 29;10(7):1049. [Abstract]; ATDC5 cells were incubated with 1 mM P* or penicillamine (Peni) for 1 h followed by stimulation with IL-1β/IFN-γ for 24 h. IL-6 levels were determined by ELISA and normalized against stimulated cells (Stim) set to 100 %.

: Penicillamine purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2021 Jun 29;10(7):1049. [Abstract]; Primary human chondrocytes from OA patients were preincubated for 1 h with 0.5 mM P* or penicillamine (Peni) and stimulated for 24 h with IL-1β. IL-6 mRNA levels were determined by qPCR with SDHA as a reference gene and normalized to Stim (100 %).

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
KB 3-1	IC₅₀	4.04 mM Compound: D-penicillamine	Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay	[PMID: 21657271]
KB-V1	IC₅₀	6.93 mM Compound: D-penicillamine	Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay	[PMID: 21657271]
Sf21	IC₅₀	> 1000 μM Compound: D-Penicillamine	Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
Sf21	IC₅₀	> 1000 μM Compound: D-Penicillamine	Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]

In Vitro

Penicillamine (D-(-)-Penicillamine) (5 mg; 7 d; CD4⁺ and CD⁺ splenocytes) promotes cellular immune responses^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3]

Cell Line:	CD4⁺ and CD⁺ splenocytes
Concentration:	5 mg
Incubation Time:	7 days
Result:	Increased IL-4 and IFN-γ mRNA expression in response to high dose treatment and remained high in both CD4⁺ and CD⁺ splenocytes.

In Vivo

Penicillamine (D-(-)-Penicillamine) (200 mg/kg; i.g.; daily, for 3, 10 and 14 d; tx mice and DL mice) increases serum free copper concentration^[1].
Penicillamine (200 mg/kg; i.g.; daily, for 3, 10 and 14 d; tx mice and DL mice) increases ATP7A and CTR1 mRNA expression in the brain of tx mice^[1].
Penicillamine (200 mg/kg; i.g.; daily, for 3, 10 and 14 d; tx mice and DL mice) induces oxidative-stress in the central nervous system^[1].
Penicillamine (0.1-250 mg/kg; i.p.; once, for 90 min; male NMRI mice) has binaural phase effect on seizure^[2].
Penicillamine (5 mg/kg; i.v.; daily, for 8 weeks; male BN rats) prevents the onset of autoimmunity at a low dose^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Toxic milk mutant mice (tx mice) and DL mice^[1]
Dosage:	200 mg/kg
Administration:	Oral gavage; daily, for 3, 10 and 14 days
Result:	Increased the free copper concentrations in the tx mice serum on the 3rd day.

Animal Model:	Toxic milk mutant mice (tx mice) and DL mice^[1]
Dosage:	200 mg/kg
Administration:	Oral gavage; daily, for 3, 10 and 14 days
Result:	Increased the mRNA expression of ATP7A by 4-fold. Increased CTR1 mRNA expression by 6.9-fold in the cortex and 9.1-fold in the basal ganglia of tx mice.

Animal Model:	Toxic milk mutant mice (tx mice) and DL mice^[1]
Dosage:	200 mg/kg
Administration:	Oral gavage; daily, for 3, 10 and 14 days
Result:	Increased the concentration of MDA and decreased GSH/GSSG ratios through nitric oxide/NMDA pathways.

Animal Model:	Male NMRI mice^[2]
Dosage:	0.1, 0.5, 1, 10, 100, 150 and 250 mg/kg
Administration:	Intraperitoneal injection; once, for 90 minutes
Result:	Had anticonvulsant effects at a low dose (0.5 mg/kg) and had anticonvulsant effects at a high dose (250 mg/kg). Reversed the anti- and proconvulsant effects by acute pretreatment of L-NAME (a nonselective nitric oxide synthase inhibitor) and 7-NI (a selective neuronal nitric oxide synthase inhibitor).

Animal Model:	Male BN rats^[3]
Dosage:	5 mg/kg
Administration:	Intravenous injection; daily, for 8 weeks
Result:	Inhibited IgE upregulation and prevented the onset of autoimmunity.

Clinical Trial

Molecular Weight

149.22

Formula

C₅H₁₁NO₂S

CAS No.

52-67-5

Appearance

Solid

Color

White to off-white

SMILES

N[C@H](C(C)(S)C)C(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

Penicillium

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

H₂O : 83.33 mg/mL (558.44 mM; ultrasonic and warming and heat to 60°C)

DMSO : 1.43 mg/mL (9.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.7015 mL	33.5076 mL	67.0151 mL
5 mM	1.3403 mL	6.7015 mL	13.4030 mL
10 mM	0.6702 mL	3.3508 mL	6.7015 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (670.15 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (278 KB) SDS (418 KB)

COA (247 KB) HNMR (223 KB)

Handling Instructions (2659 KB)

References

[1]. Chen DB, et, al. Penicillamine increases free copper and enhances oxidative stress in the brain of toxic milk mice. PLoS One. 2012;7(5):e37709. [Content Brief]

[2]. Rahimi N, et, al. Effects of D-penicillamine on pentylenetetrazole-induced seizures in mice: involvement of nitric oxide/NMDA pathways. Epilepsy Behav. 2014 Oct;39:42-7. [Content Brief]

[3]. Masson MJ, et, al. Tolerance induced by low dose D-penicillamine in the brown Norway rat model of drug-induced autoimmunity is immune-mediated. Chem Res Toxicol. 2004 Jan;17(1):82-94. [Content Brief]

[4]. Ishak R, et, al. Penicillamine revisited: historic overview and review of the clinical uses and cutaneous adverse effects. Am J Clin Dermatol. 2013 Jun;14(3):223-33. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	6.7015 mL	33.5076 mL	67.0151 mL	167.5379 mL
DMSO / H₂O	5 mM	1.3403 mL	6.7015 mL	13.4030 mL	33.5076 mL
H₂O	10 mM	0.6702 mL	3.3508 mL	6.7015 mL	16.7538 mL
	15 mM	0.4468 mL	2.2338 mL	4.4677 mL	11.1692 mL
	20 mM	0.3351 mL	1.6754 mL	3.3508 mL	8.3769 mL
	25 mM	0.2681 mL	1.3403 mL	2.6806 mL	6.7015 mL
	30 mM	0.2234 mL	1.1169 mL	2.2338 mL	5.5846 mL
	40 mM	0.1675 mL	0.8377 mL	1.6754 mL	4.1884 mL
	50 mM	0.1340 mL	0.6702 mL	1.3403 mL	3.3508 mL
	60 mM	0.1117 mL	0.5585 mL	1.1169 mL	2.7923 mL
	80 mM	0.0838 mL	0.4188 mL	0.8377 mL	2.0942 mL
	100 mM	0.0670 mL	0.3351 mL	0.6702 mL	1.6754 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Penicillamine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Penicillamine52-67-5D-(-)-PenicillamineCuproptosisDrug Metabolitenitric oxidesynthase inhibitorspentylenetetrazole seizure miceimmune modulatorrheumatoid arthritiscystinuriaInhibitorinhibitorinhibit

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Penicillamine (Synonyms: D-(-)-Penicillamine)

Customer Review

Other Forms of Penicillamine:

Penicillamine Related Antibodies

3 Publications Citing Use of MCE Penicillamine

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

Penicillamine Related Antibodies

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Complete Stock Solution Preparation Table

Penicillamine Related Classifications

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