Cat. No.: HY-16576A

SMI-4a

(Synonyms: TCS-PIM-1-4a)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

SMI-4a (TCS-PIM-1-4a) is a poten, selective, cell-permeable and ATP-competitive Pim-1 inhibitor with an IC₅₀ of 24 μM and a K_i of 0.6 μM. SMI-4a also inhibits Pim-2 (IC₅₀ of 100 μM), and does not significantly inhibit the other serine/threonine- or tyrosine-kinases. SMI-4a has anticancer activity.

For research use only. We do not sell to patients.

Purity: 99.85%
CAS No.: 438190-29-5
Formula: C₁₁H₆F₃NO₂S
Molecular Weight:273.23

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

SMI-4a Chemical Structure

CAS No.: 438190-29-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

PIM1

24 μM (IC₅₀)

PIM1

0.6 μM (Ki)

PIM2

100 μM (IC₅₀)

All Pim Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
DLD-1	IC₅₀	17.8 μM Compound: 4	Inhibition of growth of DLD-1 cell line after 96 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of DLD-1 cell line after 96 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
DLD-1	IC₅₀	31.4 μM Compound: 4	Inhibition of growth of DLD-1 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of DLD-1 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
DLD-1	IC₅₀	37.4 μM Compound: 4	Inhibition of growth of DLD-1 cell line after 72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of DLD-1 cell line after 72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
DLD-1	IC₅₀	40.1 μM Compound: 4	Inhibition of growth of DLD-1 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of DLD-1 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-116	IC₅₀	36.8 μM Compound: 4	Inhibition of growth of HCT116 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT116 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-116	IC₅₀	45.5 μM Compound: 4	Inhibition of growth of HCT116 cell line after 96 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT116 cell line after 96 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-116	IC₅₀	48.8 μM Compound: 4	Inhibition of growth of HCT116 cell line after 72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT116 cell line after 72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-116	IC₅₀	91.7 μM Compound: 4	Inhibition of growth of HCT116 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT116 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-8	IC₅₀	100 μM Compound: 4	Inhibition of growth of HCT-8 cell line after 72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT-8 cell line after 72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HCT-8	IC₅₀	82.2 μM Compound: 4	Inhibition of growth of HCT-8 cell line after 96 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HCT-8 cell line after 96 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HL-60	GI₅₀	4.9 μM Compound: SMI-4a	Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay Antiproliferative activity against human HL-60 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay	[PMID: 34958208]
HT-29	IC₅₀	31.4 μM Compound: 4	Inhibition of growth of HT-29 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HT-29 cell line after 72+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HT-29	IC₅₀	38.8 μM Compound: 4	Inhibition of growth of HT-29 cell line after 72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HT-29 cell line after 72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HT-29	IC₅₀	59.7 μM Compound: 4	Inhibition of growth of HT-29 cell line after 96 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HT-29 cell line after 96 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
HT-29	IC₅₀	71.7 μM Compound: 4	Inhibition of growth of HT-29 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO Inhibition of growth of HT-29 cell line after 24+72 hours of exposure time with the compound dissolved in DMSO	[PMID: 15993594]
MOLM-13	GI₅₀	2.16 μM Compound: SMI-4a	Antiproliferative activity against human MOLM-13 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay Antiproliferative activity against human MOLM-13 cells assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay	[PMID: 34958208]
Mononuclear cell line	GI₅₀	8.35 μM Compound: SMI-4a	Antiproliferative activity against AML patient derived mononuclear cells of assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay Antiproliferative activity against AML patient derived mononuclear cells of assessed as cell growth inhibition incubated for 72 hrs by CCK8 assay	[PMID: 34958208]
PC-3	IC₅₀	17 μM Compound: 4a	Antiproliferative activity against human PC3 cells after 48 hrs by MTS assay Antiproliferative activity against human PC3 cells after 48 hrs by MTS assay	[PMID: 19072652]

In Vitro

SMI-4a (0.5 μM; 1 hour; HEK-293T cells) treatment attenuates the autophosphorylation of tagged Pim-1 in intact cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HEK-293T cells
Concentration:

0.5 µM
Incubation Time:

1 hour
Result:

Caused a dose-dependent reduction in Pim-1-induced 4E-BP1 phosphorylation, with an IC₅₀ of approximately 125 nM.

Chemical Information

CAS No. 438190-29-5
Appearance Solid
Molecular Weight 273.23
Formula C₁₁H₆F₃NO₂S
Color White to off-white
SMILES

O=C(NC/1=O)SC1=C/C2=CC=CC(C(F)(F)F)=C2
Synonyms

TCS-PIM-1-4a
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (1)

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Journal Impact Factor

J Virol

Neuronal expression of herpes simplex virus-1 VP16 protein induces pseudorabies virus escape from silencing and reactivation. [Abstract]

2024 Jun 13:e0056124. PMID: 38869285

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (365.99 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.6599 mL

18.2996 mL

36.5992 mL

5 mM

0.7320 mL

3.6599 mL

7.3198 mL

10 mM

0.3660 mL

1.8300 mL

3.6599 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 2 mg/mL (7.32 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 20% HP-β-CD in Saline

Solubility: 5 mg/mL (18.30 mM); Suspended solution; Need ultrasonic

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xia Z, et al. Synthesis and evaluation of novel inhibitors of Pim-1 and Pim-2 protein kinases. J Med Chem. 2009 Jan 8;52(1):74-86. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.6599 mL	18.2996 mL	36.5992 mL	91.4980 mL
	5 mM	0.7320 mL	3.6599 mL	7.3198 mL	18.2996 mL
	10 mM	0.3660 mL	1.8300 mL	3.6599 mL	9.1498 mL
	15 mM	0.2440 mL	1.2200 mL	2.4399 mL	6.0999 mL
	20 mM	0.1830 mL	0.9150 mL	1.8300 mL	4.5749 mL
	25 mM	0.1464 mL	0.7320 mL	1.4640 mL	3.6599 mL
	30 mM	0.1220 mL	0.6100 mL	1.2200 mL	3.0499 mL
	40 mM	0.0915 mL	0.4575 mL	0.9150 mL	2.2875 mL
	50 mM	0.0732 mL	0.3660 mL	0.7320 mL	1.8300 mL
	60 mM	0.0610 mL	0.3050 mL	0.6100 mL	1.5250 mL
	80 mM	0.0457 mL	0.2287 mL	0.4575 mL	1.1437 mL
	100 mM	0.0366 mL	0.1830 mL	0.3660 mL	0.9150 mL

Keywords

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SMI-4a

Epigenetics Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Anti-Cancer Compound Library

Covalent Screening Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) SMI-4a

All Pim Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

SMI-4a

Epigenetics Compound Library

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Anti-Cancer Compound Library

Covalent Screening Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Protein Kinase Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) SMI-4a

All Pim Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

SMI-4a Related Classifications

Cat. No.: HY-16576A