2. Others Neuronal Signaling
  3. Fluorescent Dye Cholinesterase (ChE)
  4. AD-BChE/HClO

AD-BChE/HClO is a dual-target two-photon fluorescent probe. AD-BChE/HClO can release 4-methylumbelliferone via butyrylcholinesterase-mediated hydrolysis of the ester bond at position 7, as well as hypochlorous acid-mediated thiocarbonyl oxidation. AD-BChE/HClO enables two-photon imaging in nerve cells and mouse brain tissues via tail vein injection. AD-BChE/HClO can be used for the research of Alzheimer's disease.

For research use only. We do not sell to patients.

AD-BChE/HClO

AD-BChE/HClO Chemical Structure

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AD-BChE/HClO Related Antibodies

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Description

AD-BChE/HClO is a dual-target two-photon fluorescent probe. AD-BChE/HClO can release 4-methylumbelliferone via butyrylcholinesterase-mediated hydrolysis of the ester bond at position 7, as well as hypochlorous acid-mediated thiocarbonyl oxidation. AD-BChE/HClO enables two-photon imaging in nerve cells and mouse brain tissues via tail vein injection. AD-BChE/HClO can be used for the research of Alzheimer's disease[1].

IC50 & Target

BChE

 

In Vitro

AD-BChE/HClO (AND logic gate probe, Probe S1) (10 μM; up to 80 min) acts as an AND logic gate probe, producing strong fluorescence only in the simultaneous presence of BChE and HClO, with detection limits of 8.28 U/L for BChE and 3.09 μM for HClO in cell-free biochemical assays[1].
AD-BChE/HClO (AND logic gate probe, Probe S1) (0-100 μM; 24 h for cytotoxicity, 40 min for imaging) is non-cytotoxic to PC12 cells at concentrations up to 100 μM, and it can specifically detect both exogenous and endogenous HClO, upregulated BChE, and differentiate Aβ1-42-induced or glutamate-induced AD model PC12 cells from normal cells via two-photon fluorescence imaging[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AD-BChE/HClO Related Antibodies

Cell Cytotoxicity Assay[1]

Cell Line: PC12 cells
Concentration: 0-100 μM (cytotoxicity assay); 10 μM (imaging assays); 50-200 μM HClO; 200-500 μM hydrocortisone; 5-20 μM Aβ1-42; 5-20 mM L-glutamate
Incubation Time: 24 h (cytotoxicity assay; L-glutamate stimulation); 40 min (probe incubation for imaging); 30 min (HClO stimulation); 48 h (hydrocortisone stimulation; Aβ1-42 stimulation)
Result: Maintained PC12 cell viability above 80% after 24 h incubation at concentrations from 0 to 100 μM.
Showed increased fluorescence intensity with increasing exogenous HClO concentration.
Increased fluorescence due to LPS-induced endogenous HClO production, which was reduced by ABH treatment.
Increased fluorescence intensity due to hydrocortisone-induced BChE upregulation, which was reduced more significantly by BChE inhibitor iso-OMPA than by AChE inhibitor donepezil.
Showed 2-3 fold higher fluorescence intensity in Aβ1-42-induced and glutamate-induced AD cell models than in normal cells, which was reduced by treatment with ABH or iso-OMPA.
Molecular Weight

260.31

Formula

C14H12O3S
SMILES

CC1=CC(OC2=CC(OC(C3CC3)=O)=CC=C12)=S
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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AD-BChE/HClO Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AD-BChE/HClOFluorescent DyeCholinesterase (ChE)neural cellsAβ1-42glutamatehypochlorous acidbutyrylcholinesterasemouse brainsPC12 cells4-methylumbelliferonealzheimer’s diseaseInhibitorinhibitorinhibit

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