AD-BChE/HClO

Guidelines (The following are our recommended protocols; this is a guideline and should be modified according to your specific needs).
1. Stock Solution Preparation
1.1 Solvent: For most dyes, organic solvents such as anhydrous DMSO are typically used for dissolution.
1.2 Concentration Recommendation: Generally, a high concentration of 1-10 mM stock solution is recommended.
1.3 Storage Conditions: Store at -20 ℃ or -80 ℃ protected from light and avoid repeated freeze-thaw cycles.
2. Working Solution Preparation
2.1 Diluent: Serum-free culture medium or PBS is typically used. Proteins and esterases in serum may interfere with staining or cause dye hydrolysis.
2.2 Working Concentration: 10 μM
Note: Please adjust the dye working solution concentration according to your actual situation and prepare fresh before use.
3. Cell Staining
3.1 Culture cells on sterile coverslips.
3.2 Remove the coverslips from the culture medium and aspirate excess medium.
3.3 Add working solution, gently shake to completely cover the cells, and incubate at 37 ℃ for 40 min.
3.4 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope or flow cytometry.
Note: If flow cytometry is required, the cells should be resuspended with trypsin before staining.
AD-BChE/HClO (Probe S1) (10 μM; up to 80 min) acts as an AND logic gate probe, producing strong fluorescence only in the simultaneous presence of BChE and HClO, with detection limits of 8.28 U/L for BChE and 3.09 μM for HClO in cell-free biochemical assays^[1].
AD-BChE/HClO (0-100 μM; 24 h) is non-cytotoxic to PC12 cells at concentrations up to 100 μM, and it can specifically detect both exogenous and endogenous HClO, upregulated BChE, and differentiate Aβ_1-42-induced or glutamate-induced AD model PC12 cells from normal cells via two-photon fluorescence imaging^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.