Antibacterial agent 330 

Antibacterial agent 330 is an antibacterial agent. Antibacterial agent 330 triggers ROS accumulation, forms DNA supramolecular complex by intercalation to block DNA replication and inhibits LDH to
disturb metabolism, and further prompts bacterial cell rupture to induce the leakage of intracellular content, ultimately causing bacterial death. Antibacterial agent 330 displays antibacterial activity and promotes wound healing in both G. Mellonella larval and murine wound infection models. Antibacterial agent 330 can be used for the research of bacterial infections^[1].

Antibacterial agent 330 (Compound I-10b) potently inhibits the growth of multiple Gram-positive and Gram-negative bacterial strains (MICs =
0.0007-0.0026 mM), with the lowest MIC of 0.0003 mM against Enterococcus faecalis, and also inhibits clinically isolated Enterococcus faecalis strains at low concentrations^[1].
Antibacterial agent 330 (4×-8× MIC; up to 8 h) exhibits concentration-dependent bactericidal activity against Staphylococcus aureus 25923 and Enterococcus faecalis, with significant CFU reduction observed at 8× MIC within 6 hours^[1].
Antibacterial agent 330 (14 days) shows low potential to induce bacterial resistance in Staphylococcus aureus 25923 and Enterococcus faecalis, and potently inhibits sulfamethoxazole-resistant bacterial strains at low concentrations (0.0007-0.0055 mM)^[1].
Antibacterial agent 330 (32×-64× MIC; 4 h) exhibits negligible hemolytic toxicity^[1].
Antibacterial agent 330 (5-40 μg/mL) shows no significant cytotoxicity to human normal LO2 hepatic cells and Beas-2b lung epithelial cells^[1].
Antibacterial agent 330 (0.5×-16× MIC) effectively inhibits and eradicates biofilms of Staphylococcus aureus 25923 and Enterococcus faecalis in a concentration-dependent manner, with over 40% eradication of Staphylococcus aureus 25923 biofilm at 16× MIC^[1].
Antibacterial agent 330 (1×-16× MIC) increases membrane permeability in Staphylococcus aureus 25923 and Enterococcus faecalis^[1].
Antibacterial agent 330 (0.5×-8× MIC) induces concentration-dependent protein leakage from Staphylococcus aureus 25923 and Enterococcus faecalis, indicating bacterial membrane damage^[1].
Antibacterial agent 330 (0.5×-16× MIC) triggers concentration-dependent intracellular ROS accumulation in Staphylococcus aureus 25923 and Enterococcus faecalis^[1].
Antibacterial agent 330 (4×-16× MIC) inhibits LDH activity in Staphylococcus aureus 25923 and Enterococcus faecalis by ~45% at 16× MIC^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Antibacterial agent 330 (Compound I-10b) (0.007-0.014 mM; injection; single dose) exhibits potent antibacterial efficacy against S. aureus 25923 in Galleria mellonella larvae, with the 0.007 mM dose yielding a 90% 8-day survival rate and the 0.014 mM dose maintaining near-complete survival^[1].
Antibacterial agent 330 (4× MIC; topical; once daily; 6 days) effectively promotes wound healing and reduces S. aureus 25923 bacterial load in a murine wound infection model, while supporting normal mouse weight gain^[1].
Antibacterial agent 330 (5 mg/kg/day; daily; 3 days) exhibits good in vivo biosafety in Kunming mice, with no histopathological damage observed in major organs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

759.16

C₃₄H₃₆ClN₄O₁₀PS

3031427-16-1

CCOP(OCC)(C(C1=CC(OC)=C(C2=C[N+]3=C(C4=CC5=C(OCO5)C=C4CC3)C=C12)O)NC6=CC=C(C=C6)S(=O)(NC7=NOC(C)=C7)=O)=O.[Cl-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zhou Y, et al. Novel aminophosphoryl hybridization of natural berberine and heterocyclic azoles with large potential to combat global increasing bacterial resistance. Bioorg Chem. Published online March 4, 2026.  [Content Brief]