1. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease JAK/STAT Signaling Protein Tyrosine Kinase/RTK Apoptosis
  2. Reactive Oxygen Species EGFR Apoptosis
  3. Anticancer agent 69

Anticancer agent 69 (Compound 34), a potent and selective anticancer agent , potently and selectively inhibits human prostate cancer cell line PC3 (IC50=26 nM). Anticancer agent 69 increases ROS level, down-regulates EGFR and induces apoptosis.

For research use only. We do not sell to patients.

Anticancer agent 69 Chemical Structure

Anticancer agent 69 Chemical Structure

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Description

Anticancer agent 69 (Compound 34), a potent and selective anticancer agent , potently and selectively inhibits human prostate cancer cell line PC3 (IC50=26 nM). Anticancer agent 69 increases ROS level, down-regulates EGFR and induces apoptosis[1].

In Vitro

Anticancer agent 69 (Compound 34, 0-20 μM approximately, 72 h) has selective anticancer activity in PC3 cells with an IC50 value of 26 nM against other cancer cell lines and normal cell lines[1].
Anticancer agent 69 (1-7 days) inhibits the cell viabilities of PC3 cells and PC9 cells in a time- and dose-dependent manne[1].
Anticancer agent 69 (0-100 nM, 7 days) forms fewer and smaller colonies compared those with DMSO-treated cells[1].
Anticancer agent 69 (0-800 nM, 34/72 h) increases ROS production, and further stimulates Prx I-III expression, phosphorylation level of MAPK ‘s downstream proteins, expression of apoptosis-related proteins[1].
Anticancer agent 69 (0-500 nM, 72 h) decreases the level of EGFR and phosphorylation level of its downstream protein (ERK, AKT), and induces PC3 cells and PC9 cells apoptosis in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: PC3, PC9
Concentration: 0-800 nM
Incubation Time: 72 h
Result: Stimulated Prx I-III expression, phosphorylation level of MAPK ‘s downstream proteins (P38, JNK), expression of apoptosis-related proteins (Bax, cleaved-Caspased 3).
Inhibited the level of EGFR and its downstream protein (p-ERK, p-AKT). Increased pro-apoptotic proteins (Bax and P53) expression and reduces anti-apoptotic protein (Bcl-2) expression.

Cell Proliferation Assay[1]

Cell Line: PC3, PC9
Concentration: 0-100 nM
Incubation Time: 7 days
Result: Formed fewer and smaller colonies compared those with DMSO-treated cells.

Cell Viability Assay[1]

Cell Line: Cancer cell lines (PC3, MGC-803, PC9, EC9706, SMMC-7721), normal cell lines (Het-1A, L02 and GES-1)
Concentration: 0-20 μM approximately
Incubation Time: 72 h
Result: Inhibited proliferation of multiple cancer cell lines with IC50 values of 26 nM (PC3), 557 nM (MGC-803), 148 nΜ (PC9), 3.99 μΜ (EC9706), 844 nΜ (SMMC-7721), respectively.
Displayed good selectivity against normal cell lines with IC50 values of > 20 μΜ (L02), > 5 μΜ (Het-1A), 1.57 μΜ (GES-1), respectively.
Molecular Weight

398.53

Formula

C19H26N8S

SMILES

[H]/C(C1=CC=CC=N1)=N\NC2=NC(SCCC)=NC3=C2N=NN3CCCCCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Anticancer agent 69
Cat. No.:
HY-146170
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