2. NF-κB
  3. NF-κB
  4. Aristolochic acid A sodium

Aristolochic acid A sodium  (Synonyms: Aristolochic acid I sodium; TR 1736 sodium)

Cat. No.: HY-N0510A Purity: 99.93%
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Aristolochic acid A (Aristolochic acid I) sodium is the main component of plant extract Aristolochic acids, which are found in various herbal plants of genus Aristolochia and Asarum. Aristolochic acid A sodium significantly reduces both activator protein 1 (AP-1) and NF-κB activities. Aristolochic acid A sodium reduces BLCAP gene expression in human cell lines.

For research use only. We do not sell to patients.

CAS No. : 10190-99-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
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Customer Review

Based on 10 publication(s) in Google Scholar

Other Forms of Aristolochic acid A sodium:

Aristolochic acid A sodium Related Antibodies

Top Publications Citing Use of Products

    Aristolochic acid A sodium purchased from MedChemExpress. Usage Cited in: Nature. 2024 Jul;631(8020):459-466.  [Abstract]

    FLIPR Ca2+ assay responses in wild-type (WT) TAS2R14 and mutants in pockets 1, 2 and 3, following Aristolochic acid A (150 μM) stimulation. The negative logarithmic half-maximal effective concentration (pEC50) and the maximum response (Emax) values were calculated from the concentration–response curves.

    Aristolochic acid A sodium purchased from MedChemExpress. Usage Cited in: Precis Clin Med. 2022 Sep 22;5(4):pbac023.

    Aristolochic acid A (AAI, 2 mg/kg/day). Body weight of mice in Control, AAI-4w, and AAI-8w groups.

    Aristolochic acid A sodium purchased from MedChemExpress. Usage Cited in: Precis Clin Med. 2022 Sep 22;5(4):pbac023.

    Aristolochic acid A (AAI, 2 mg/kg/day, 4 or 8 weeks). H&E staining showed pathological changes of mice livers after AAI treatment, scale bar = 100 μm.

    Aristolochic acid A sodium purchased from MedChemExpress. Usage Cited in: Precis Clin Med. 2022 Sep 22;5(4):pbac023.

    Aristolochic acid A (AAI, 2 mg/kg/day, 4 or 8 weeks). Liver/body weight ratio after AAI treatment (n = 5, ***P < 0.001, ns = not significant). Effects of AAI on the levels of serum ALT and AST in the indicated groups (n = 5, *P < 0.05, **P < 0.01, ns = not significant).

    Aristolochic acid A sodium purchased from MedChemExpress. Usage Cited in: Precis Clin Med. 2022 Sep 22;5(4):pbac023.

    Aristolochic acid A (AAI, 2 mg/kg/day, 4 or 8 weeks). IF staining of p-STAT3 (red) in the three groups, and the nuclei were stained by Hoechst (blue), scale bar = 50 µm.

    Aristolochic acid A sodium purchased from MedChemExpress. Usage Cited in: Precis Clin Med. 2022 Sep 22;5(4):pbac023.

    Aristolochic acid A (AAI, 2 mg/kg/day, 4 or 8 weeks). IF staining of Bcl-2 (green) and Bax (Red) to detect endothelial cell apoptosis in the three groups, the nucleus was labelled by Hoechst (blue), scale bar = 40 µm.

    Aristolochic acid A sodium purchased from MedChemExpress. Usage Cited in: Precis Clin Med. 2022 Sep 22;5(4):pbac023.

    Aristolochic acid A (AAI, 2 mg/kg/day, 4 or 8 weeks). Western blot was used to detect the expression of apoptotic signaling pathway-related markers cleaved-caspase 3, Bax, and Bcl-2 in liver tissues after AAI treatment.

    View All NF-κB Isoform Specific Products:

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    NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Aristolochic acid A (Aristolochic acid I) sodium is the main component of plant extract Aristolochic acids, which are found in various herbal plants of genus Aristolochia and Asarum. Aristolochic acid A sodium significantly reduces both activator protein 1 (AP-1) and NF-κB activities. Aristolochic acid A sodium reduces BLCAP gene expression in human cell lines[1].

    In Vitro

    Aristolochic acid A (150, 200 μM, 24 hours) sodium inhibits the cell viabilities of kidney cells HEK293 and HK-2[1].
    Aristolochic acid A (100, 200 μM, 24 hours) sodium causes a concentration-dependent decrease in bladder cancer-associated protein (BLCAP) mRNA levels in kidney cells (HEK 293 and HK-2) , and bladder cancer cell line (HT-1376)[1].
    Aristolochic acid A (100, 200 μM, 24 hours) sodium weakens the BLCAP protein signals in a dose-dependent manner in both HEK293 and HT-1376 cells[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Aristolochic acid A sodium Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Kidney cells (HEK 293 and HK-2)
    Concentration: 50, 100, 150, 200 μM
    Incubation Time: 24 hours
    Result: The cell viabilities of HEK293 and HK-2 were lower than that of vehicle-treated cultures until 150 μM and 200 μM.

    RT-PCR[1]

    Cell Line: Kidney cells (HEK 293 and HK-2), and bladder cancer cell line (HT-1376)
    Concentration: 100, 200 μM
    Incubation Time: 24 hours
    Result: Down regulated the levels of BLCAP mRNA.

    Western Blot Analysis[1]

    Cell Line: HEK293 and HT-1376 cells
    Concentration: 100, 200 μM
    Incubation Time: 24 hours
    Result: Reduced the BLCAP protein expression in a dose-dependent manner.
    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment    .

    Aristolochic acid A can be used to induce nephrotoxicity models. Pharmacokinetic studies show that in male C57BL/6 mice, the concentration of Aristolochic acid A in the kidneys is higher than in the liver 30 minutes after intraperitoneal administration (10 mg/kg). The plasma concentration of Aristolochic acid A also peaks approximately 30 minutes after administration[2].

    Induction of Nephrotoxicity [2][3]
    Background
    The pathogenesis of Aristolochic acid Anephropathy remains unclear. Research shows that Aristolochic acid A mainly damages renal tubular epithelial cells and renal tules, leading to interstitial fibrosis, thereby producing nephrotoxicity.
    Specific Modeling Methods
    Mice: C57BL/6J • 8 weeks of age
    Administration: 10 mg/kg • i.p. • 3-5 days[3]
    Modeling Indicators
    Pathology changs: H&E stain shows renal tissue structure is disordered, glomerular edema, pyknosis, and proximal tubule epithelial cell shedding
    Biochemistry changes: Scr and blood urea nitrogen (BUN) contents increases

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    363.25

    Formula

    C17H10NNaO7
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    O=C(O[Na])C1=CC2=C(OCO2)C3=C4C(C(OC)=CC=C4)=CC([N+]([O-])=O)=C31
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 10 mg/mL (27.53 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7529 mL 13.7646 mL 27.5293 mL
    5 mM 0.5506 mL 2.7529 mL 5.5058 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.93%

    SDS (584 KB)

    COA (258 KB) HNMR (265 KB) LCMS (338 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.7529 mL 13.7646 mL 27.5292 mL 68.8231 mL
    5 mM 0.5506 mL 2.7529 mL 5.5058 mL 13.7646 mL
    10 mM 0.2753 mL 1.3765 mL 2.7529 mL 6.8823 mL
    15 mM 0.1835 mL 0.9176 mL 1.8353 mL 4.5882 mL
    20 mM 0.1376 mL 0.6882 mL 1.3765 mL 3.4412 mL
    25 mM 0.1101 mL 0.5506 mL 1.1012 mL 2.7529 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Aristolochic acid A sodium Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Aristolochic acid A10190-99-5Aristolochic acid ITR 1736TR1736TR-1736NF-κBNuclear factor-κBNuclear factor-kappaBbiochemical assayreagentInhibitorinhibitorinhibit

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