1. Metabolic Enzyme/Protease
  2. Phosphatase
  3. ARL67156 triethylamine

ARL67156 triethylamine  (Synonyms: FPL 67156 triethylamine)

Cat. No.: HY-103265D Purity: ≥98.0%
COA Handling Instructions

ARL67156 (FPL 67156) triethylamine is a selective small is a selective samll molecular inhibitor, targeting to ecto-ATPase, CD39, and CD73. ARL67156 triethylamine is also a competitive inhibitor of NTPDase1 (CD39), NTPDase3 and NPP1, with Kis of 11, 18 and 12?μM, respectively. ARL67156 triethylamine can be used in the research of disease like calcific aortic valve disease, asthma.

For research use only. We do not sell to patients.

ARL67156 triethylamine Chemical Structure

ARL67156 triethylamine Chemical Structure

Size Price Stock Quantity
1 mg USD 180 In-stock
5 mg USD 650 In-stock
10 mg USD 1040 In-stock
25 mg USD 2100 In-stock
50 mg USD 3360 In-stock
100 mg USD 5350 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of ARL67156 triethylamine:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

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Description

ARL67156 (FPL 67156) triethylamine is a selective small is a selective samll molecular inhibitor, targeting to ecto-ATPase, CD39, and CD73. ARL67156 triethylamine is also a competitive inhibitor of NTPDase1 (CD39), NTPDase3 and NPP1, with Kis of 11, 18 and 12?μM, respectively. ARL67156 triethylamine can be used in the research of disease like calcific aortic valve disease, asthma[1][2].

IC50 & Target

Ki: 11 μM (NTPDase1), 18 μM (NTPDase3), 12 μM (NPP1)[1]; 0.97 μM (CD39), 0.45 μM (CD73)[8]

In Vitro

ARL67156 triethylamine (1-100 μM) potentiates neurogenic contractions in a concentration-dependent manner[4].
? ARL67156 triethylamine (10 μg/mL, 24 h) increases the surface expression of CXCR3 on ATP-treated HMC-1 cells[5].
? ARL67156 triethylamine (30 μM, 5s) potentiates the norepinephrine release promoted by ATP in guinea pig heart synaptosomes[6].
? ARL67156 triethylamine (100 μM, 4h) significantly decreases HIV-1replication in macrophages[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ARL67156 triethylamine (1.1 μg/kg/day, administered with osmotic pumps implanted subcutaneously, for 28 days) prevents the development of calcific aortic valve disease in Warfarin (HY-B0687)-treated rats[2].
? ARL67156 triethylamine (intraperitoneal injection, 2?mg/kg) prevents the increase of serum adenosine concentration induced by Fructose 1,6-bisphosphate (FBP)[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Warfarin-induced mineralization rat model[2]
Dosage: 1.1 μg/kg/day
Administration: Administered with osmotic pumps implanted subcutaneously, for 28 days
Result: Prevented the development of aortic stenosis by lowering the level of apoptosis and mineralization of the aortic valve/aorta.
Normalized the level of pAkt (an important kinase involved in the survival pathway).
Animal Model: C57BL/6 mice[3]
Dosage: 2 mg/kg
Administration: Intraperitoneal injection, 1  h before administration of FBP (100 mg/kg)
Result: Completely abolished the anti-inflammatory effects of FBP (observed by the neutrophil infiltration, hyperalgesia and oedema of the joint).
Molecular Weight

1154.23

Formula

C15H24Br2N5O12P3.(4.3C6H15N)

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1[C@@H](O[C@H](COP(OP(C(Br)(Br)P(O)(O)=O)(O)=O)(O)=O)[C@H]1O)N2C3=C(C(N(CC)CC)=NC=N3)N=C2.CCN(CC)CC.[4.3]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Methanol : 125 mg/mL (108.30 mM; Need ultrasonic)

DMSO : 100 mg/mL (86.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (86.64 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8664 mL 4.3319 mL 8.6638 mL
5 mM 0.1733 mL 0.8664 mL 1.7328 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (2.17 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O / Methanol 1 mM 0.8664 mL 4.3319 mL 8.6638 mL 21.6595 mL
5 mM 0.1733 mL 0.8664 mL 1.7328 mL 4.3319 mL
10 mM 0.0866 mL 0.4332 mL 0.8664 mL 2.1659 mL
15 mM 0.0578 mL 0.2888 mL 0.5776 mL 1.4440 mL
20 mM 0.0433 mL 0.2166 mL 0.4332 mL 1.0830 mL
25 mM 0.0347 mL 0.1733 mL 0.3466 mL 0.8664 mL
30 mM 0.0289 mL 0.1444 mL 0.2888 mL 0.7220 mL
40 mM 0.0217 mL 0.1083 mL 0.2166 mL 0.5415 mL
50 mM 0.0173 mL 0.0866 mL 0.1733 mL 0.4332 mL
60 mM 0.0144 mL 0.0722 mL 0.1444 mL 0.3610 mL
80 mM 0.0108 mL 0.0541 mL 0.1083 mL 0.2707 mL
Methanol 100 mM 0.0087 mL 0.0433 mL 0.0866 mL 0.2166 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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ARL67156 triethylamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ARL67156 triethylamine
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