TOTO-3
Based on 1 Customer Validation
TOTO-3 is a cyanine nucleic acid intercalating fluorescent probe that binds to both RNA and DNA (Ex=642 nm, Em=660 nm). TOTO-3 cannot enter intact cells, but can penetrate cells with permeabilized plasma membranes, staining cytoplasmic RNA and nucleoli to distinguish intact cells from damaged ones. TOTO-3 is taken up by macrophages in an ultrasound-independent manner. TOTO-3 accumulates in tumor cells via ultrasound-mediated enhancement of plasma membrane permeability. TOTO-3 supports optical imaging for real-time assessment of plasma membrane integrity, nucleic acid detection, and monitoring of ultrasound-mediated intracellular delivery processes. TOTO-3 is applicable to studies related to rectal cancer.
For research use only. We do not sell to patients.
- Purity: 85.0%
- CAS No.: 166196-17-4
- Formula: C53H62I4N6S2
- Molecular Weight:1354.85
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
TOTO-3 is a cell-impermeable dye, as it only enters and stains mouse rectal carcinoma line CMT-93 cells[1].
TOTO-3 (1 μM; 5 min at 37°C) discriminates between intact and membrane-compromised human HaCaT keratinocyte cells and human M4Beu melanoma cells, enabling selective analysis of viable cells for Ursolic acid (HY-N0140)-induced mitochondrial transmembrane potential changes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 166196-17-4
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Appearance Solid
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Molecular Weight 1354.85
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Formula C53H62I4N6S2
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Color Brown to black
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SMILES
CN1C2=CC=CC=C2S/C1=C\C=C\C3=CC=[N+](CCC[N+](C)(CCC[N+](C)(CCC[N+]4=CC=C(/C=C/C=C5SC6=C(C=CC=C6)N/5C)C7=C4C=CC=C7)C)C)C8=C3C=CC=C8.[I-].[I-].[I-].[I-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 50 mg/mL (36.90 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.7381 mL | 3.6904 mL | 7.3809 mL | 18.4522 mL |
| 5 mM | 0.1476 mL | 0.7381 mL | 1.4762 mL | 3.6904 mL | |
| 10 mM | 0.0738 mL | 0.3690 mL | 0.7381 mL | 1.8452 mL | |
| 15 mM | 0.0492 mL | 0.2460 mL | 0.4921 mL | 1.2301 mL | |
| 20 mM | 0.0369 mL | 0.1845 mL | 0.3690 mL | 0.9226 mL | |
| 25 mM | 0.0295 mL | 0.1476 mL | 0.2952 mL | 0.7381 mL | |
| 30 mM | 0.0246 mL | 0.1230 mL | 0.2460 mL | 0.6151 mL |
TOTO-3 Related Classifications
HY-D0920 Related Classifications
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