2. Epigenetics PI3K/Akt/mTOR
  3. AMPK
  4. Buformin

Buformin (1-Butylbiguanide), a potent AMPK activator, acts as an orally active biguanide antidiabetic agent. Buformin decreases hepatic gluconeogenesis and lowers blood glucose production in vivo. Buformin also has anti-cancer activities and is applied in cancer study (such as, cervical cancer and breast cancer, et al).

At equivalent molar concentrations, both the salt and free forms of a compound exhibit comparable biological activity. Nevertheless, the salt form (Buformin hydrochloride) usually boasts enhanced water solubility and stability.

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Buformin Chemical Structure

Buformin Chemical Structure

CAS No. : 692-13-7

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Buformin Related Antibodies

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Description

Buformin (1-Butylbiguanide), a potent AMPK activator, acts as an orally active biguanide antidiabetic agent. Buformin decreases hepatic gluconeogenesis and lowers blood glucose production in vivo. Buformin also has anti-cancer activities and is applied in cancer study (such as, cervical cancer and breast cancer, et al)[1].

In Vitro

Buformin (0-10 mM; 5 days) inhibits SKBR3 and BT474 cells growth as a concentration-dependent manner, exhibits IC50 values of 246.7 μM and  98.6 μM for erbB-2-overexpressing SKBR3 and BT474 cells, respectively[1].
Buformin (0-3 mM; 48 hours)  increases the percentage of cells in G0/G1 phase and reduced the percentage of cells in S phase, especially in the SKBR3 cells[1].
Buformin (0-3 mM; 24 hours) suppresses RTK activation, including erbB-2 and IGF1R signaling  downstream, and Akt activation/phosphorylation is inhibited in both SKBR3 and BT474 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Buformin Related Antibodies

Cell Viability Assay[1]

Cell Line: ErbB-2-overexpressing SKBR3 and BT474 cells
Concentration: 0 μM, 1 μM, 3 μM, 10 μM, 30 μM, 100 μM, 300 μM, 1, 3, or 10 mM
Incubation Time: 5 days
Result: Reduced cell viability in erbB-2-overexpressing breast cells.

Cell Cycle Analysis[1]

Cell Line: ErbB-2-overexpressing SKBR3 and BT474 cells
Concentration: 0.5 mM; 1 mM; 3 mM
Incubation Time: 48 hours
Result: Increased cells arresting in G0/G1 phase.

Western Blot Analysis[1]

Cell Line: ErbB-2-overexpressing SKBR3 and BT474 cells
Concentration: 0 mM, 0.1 mM, 0.3 mM, 1 mM, or 3 mM
Incubation Time: 24 hours
Result: Decreased p-AMPK, p-p706S, p-ERK1/2 expression in a concentration-dependent manner.
In Vivo

Buformin (oral administation; 7.6 mmol/kg of chow; 7 days) exhibits significantly reduced tumor volumes and weights, and hinders mammary morphogenesis and proliferation in MMTV-erbB-2 transgenic mice[1]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female MMTV-erbB-2 transgenic mice[1]
Dosage: 7.6 mmol/kg
Administration: Oral administation; 7 days
Result: Inhibited mammary syngeneic tumor growth in MMTV-erbB-2 transgenic mice.
Molecular Weight

157.22

Formula

C6H15N5
CAS No.
SMILES

NC(NC(NCCCC)=N)=N
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References
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Buformin Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Buformin692-13-71-ButylbiguanideAMPKAMP-activated protein kinaseantidiabeticagentcervical cancerbreast cancerbiguanide analogType II diabeteSKBR3BT474Inhibitorinhibitorinhibit

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