C-HBrO-GGT 

C-HBrO-GGT is a sequence-activated two-photon fluorescent probe. C-HBrO-GGT exhibits sequential fluorescence activation properties: it generates fluorescence in response to hypobromous acid only after being hydrolytically activated by γ-glutamyl transpeptidase. C-HBrO-GGT enables verification of the voltage-gated chloride channel (CLC-1)-HBrO-catalase (CAT)-GGT signaling pathway at the cellular level. C-HBrO-GGT can serve as a tool to indicate the precise location of mature atherosclerotic plaques and provide early warning of plaque formation. C-HBrO-GGT is applicable to relevant research on atherosclerosis^[1].

C-HBrO-GGT reacts sequentially with GGT and HBrO in order, as confirmed by HPLC peak shifts corresponding to the formation of intermediate and final reaction products^[1].
C-HBrO-GGT (10 μM; 30 min) exhibits high sensitivity and good affinity for GGT in vitro, enabling quantitative detection of GGT activity with a detection limit of 0.076 U/L^[1].
GGT-activated C-HBrO-GGT (10 μM; overnight GGT activation) exhibits high sensitivity for HBrO in vitro, enabling quantitative detection of HBrO concentrations with a linear response across 0-12 μM^[1].
C-HBrO-GGT shows high selectivity for GGT and HBrO under physiological conditions, with optimal response to GGT at pH 7-9^[1].
C-HBrO-GGT (10 μM; 30 min) detects significantly elevated GGT activity and HBrO concentrations in ox-LDL-induced RAW 264.7 foam cells compared to untreated RAW 264.7 cells^[1].
C-HBrO-GGT uses TP fluorescence to detect elevated GGT activity and HBrO concentrations in NaBr-induced RAW 264.7 and SMMC-7721 cells, and confirms that increased HBrO indirectly elevates GGT activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C-HBrO-GGT (i.v.) enables in situ, selective two-channel fluorescence detection of elevated GGT activity and HBrO levels at atherosclerotic plaques in ApoE^-/-/HF mice, with fluorescence intensities significantly higher than those in control C57BL/6 J mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

480.46

C₂₂H₁₉F₃N₂O₅S

CSC1=CC=CC=C1C2=C3OC(C=C(C3=CC=C2NC(CCC(C(O)=O)N)=O)C(F)(F)F)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wang H, et al. Prediction of Early Atherosclerotic Plaques Using a Sequence-Activated Fluorescence Probe for the Simultaneous Detection of γ-Glutamyl Transpeptidase and Hypobromous Acid. Angew Chem Int Ed Engl. 2024;63(1):e202315861.  [Content Brief]