Formamide (deionized)
Based on 5 publication(s) in Google Scholar
Formamide deionized (Methanamide deionizde) is an effective DNA denaturant that significantly reduces the stability of DNA in buffer solutions. Formamide deionized can also be used as a decalcifying agent for rat cardiac cells. Additionally, Formamide deionized is widely used as a solvent or chemical raw material for ion compounds, resins, and plasticizers.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 75-12-7
- Formula: CH3NO
- Molecular Weight:45.04
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Formamide (deionized)
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Biological Activity
Formamide deionized (15 mol/L, 15 minutes) can induce the loss of T-tubules in rat cardiac myocytes[4]. Formamide deionized (25 mL, 10 minutes) induces DNA denaturation in apoptotic Inducer (Staurosporine (HY-15141) treated breast cancer MDA-468 cells, leukemia MOLT-4 cells, and drug-resistant subline MOLT-4/R), but has no effect on the DNA of non-apoptotic cells (actively proliferating MDA-468 and MOLT-4 cells)[5]. Formamide deionized (0.31-0.5 M, 3 hours) inhibits interkinetic nuclear migration and reduces microtubule quantity in chick embryo neuroepithelial cells[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Neuroepithelial cells in the chick embryo
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Concentration:0.1-0.5 M
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Incubation Time:3 h
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Result:Only affected mitosis which was blocked in metaphase so that mitotic figures accumulated near the neuro-coele (0.25 M). Totally inhibited interkinetic nuclear migration and affected only slightly the cell asymmetry (0.31 M). Considerably reduced the amount of cytoplasmic microtubules and that the cells became round (0.37 M). Caused all microtubules had disappeared and all cells were spherical (0.5 M).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:B6C3F1 mice[7]
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Dosage:10-160 mg/kg
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Administration:Oral Gavage (p.o.), once daily, 5 days a week for 2 weeks, 3 months, or 2 years
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Result:Resulted in a significant increase in the incidence of liver hemangiosarcoma, particularly in the 40 mg/kg and 80 mg/kg groups.
Chemical Information
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CAS No. 75-12-7
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Appearance Liquid (Density: 1.1334 g/cm³)
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Molecular Weight 45.04
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Formula CH3NO
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Color Colorless to light yellow
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SMILES
O=CN
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Synonyms
Methanamide (deionized); Formimidic acid (deionized)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (5)
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Journal Impact Factor
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Most Recent
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Redox Biol
TRPV4 drives macrophage pyroptosis via mitochondrial dysfunction and mtROS-dependent NLRP3 inflammasome activation in acute lung injury. [Abstract]2026 Jun 11:95:104255. PMID: 42314332 -
Clin Transl Med
FABP4-mediated lipid droplet accumulation drives epithelial-mesenchymal transition and aggravates alveolar epithelial barrier disruption. [Abstract]2026 Jan;16(1):e70563. PMID: 41454478 -
J Inflamm Res
Lysophosphatidylcholine 14:0 Alleviates Lipopolysaccharide-Induced Acute Lung Injury via Protecting Alveolar Epithelial Barrier by Activation of Nrf2/HO-1 Pathway. [Abstract]2024 Dec 6:17:10533-10546. PMID: 39659750 -
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Solvent & Solubility
DMSO : ≥ 166.67 mg/mL (3700.49 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Kamineni VK, et al. Layer-by-layer nanoassembly of polyelectrolytes using Formamide deionized as the working medium. Langmuir. 2007;23(14):7423-7. [Content Brief]
[2]. Blake RD, et al. Thermodynamic effects of Formamide deionized on DNA stability. Nucleic Acids Res. 1996 Jun 1;24(11):2095-103. [Content Brief]
[3]. Costanzo G, et al. Formamide deionized as the main building block in the origin of nucleic acids. BMC Evol Biol. 2007 Aug 16;7 Suppl 2(Suppl 2):S1. [Content Brief]
[4]. Brette F, et al. Validation of Formamide deionized as a detubulation agent in isolated rat cardiac cells. Am J Physiol Heart Circ Physiol. 2002 Oct;283(4):H1720-8. [Content Brief]
[5]. Frankfurt OS, Krishan A. Identification of apoptotic cells by Formamide deionized-induced dna denaturation in condensed chromatin. J Histochem Cytochem. 2001 Mar;49(3):369-78. [Content Brief]
[6]. Messier PE.Messier PE. Effects of Formamide deionized on neuroepithelial cells and on interkinetic nuclear migration in the chick embryo. J Embryol Exp Morphol. 1976 Feb;35(1):197-212. [Content Brief]
[7]. National Toxicology Program. Toxicology and carcinogenesis studies of Formamide deionized (Cas No. 75-12-7) in F344/N rats and B6C3F1 mice (gavage studies). Natl Toxicol Program Tech Rep Ser. 2008 Jul;(541):1-192. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 22.2025 mL | 111.0124 mL | 222.0249 mL | 555.0622 mL |
| 5 mM | 4.4405 mL | 22.2025 mL | 44.4050 mL | 111.0124 mL | |
| 10 mM | 2.2202 mL | 11.1012 mL | 22.2025 mL | 55.5062 mL | |
| 15 mM | 1.4802 mL | 7.4008 mL | 14.8017 mL | 37.0041 mL | |
| 20 mM | 1.1101 mL | 5.5506 mL | 11.1012 mL | 27.7531 mL | |
| 25 mM | 0.8881 mL | 4.4405 mL | 8.8810 mL | 22.2025 mL | |
| 30 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL | 18.5021 mL | |
| 40 mM | 0.5551 mL | 2.7753 mL | 5.5506 mL | 13.8766 mL | |
| 50 mM | 0.4440 mL | 2.2202 mL | 4.4405 mL | 11.1012 mL | |
| 60 mM | 0.3700 mL | 1.8502 mL | 3.7004 mL | 9.2510 mL | |
| 80 mM | 0.2775 mL | 1.3877 mL | 2.7753 mL | 6.9383 mL | |
| 100 mM | 0.2220 mL | 1.1101 mL | 2.2202 mL | 5.5506 mL |
Formamide (deionized) Related Classifications
HY-Y0842B Related Classifications
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