1. Formamide

Formamide  (Synonyms: Methanamide; Formimidic acid)

Cat. No.: HY-Y0842 Purity: ≥99.0%
COA Handling Instructions

Formamide is a denaturant of DNA which can lower DNA stability in aqueous buffer solutions. Formamide is also used as a solvent for many ionic compounds, resins and plasticizers or as a chemical feedstock.

For research use only. We do not sell to patients.

Formamide Chemical Structure

Formamide Chemical Structure

CAS No. : 75-12-7

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Description

Formamide is a denaturant of DNA which can lower DNA stability in aqueous buffer solutions. Formamide is also used as a solvent for many ionic compounds, resins and plasticizers or as a chemical feedstock[1][2].

Molecular Weight

45.04

Formula

CH3NO

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

O=CN

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (2220.25 mM; Need ultrasonic)

DMSO : 100 mg/mL (2220.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 22.2025 mL 111.0124 mL 222.0249 mL
5 mM 4.4405 mL 22.2025 mL 44.4050 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥99.0%

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 22.2025 mL 111.0124 mL 222.0249 mL 555.0622 mL
5 mM 4.4405 mL 22.2025 mL 44.4050 mL 111.0124 mL
10 mM 2.2202 mL 11.1012 mL 22.2025 mL 55.5062 mL
15 mM 1.4802 mL 7.4008 mL 14.8017 mL 37.0041 mL
20 mM 1.1101 mL 5.5506 mL 11.1012 mL 27.7531 mL
25 mM 0.8881 mL 4.4405 mL 8.8810 mL 22.2025 mL
30 mM 0.7401 mL 3.7004 mL 7.4008 mL 18.5021 mL
40 mM 0.5551 mL 2.7753 mL 5.5506 mL 13.8766 mL
50 mM 0.4440 mL 2.2202 mL 4.4405 mL 11.1012 mL
60 mM 0.3700 mL 1.8502 mL 3.7004 mL 9.2510 mL
80 mM 0.2775 mL 1.3877 mL 2.7753 mL 6.9383 mL
100 mM 0.2220 mL 1.1101 mL 2.2202 mL 5.5506 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Formamide
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HY-Y0842
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