BAY 59-9435
Based on 6 publication(s) in Google Scholar
BAY 59-9435 is a potent and selective inhibitor of Hormone Sensitive Lipase (HSL), with an IC50 of 0.023 μM.
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- Pureté: 99.92%
- CAS No.: 654059-21-9
- Formule: C14H22N2O3
- Masse moléculaire:266.34
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BAY 59-9435
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Activité biologique
IC50: 0.023 μM (HSL)[1].
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 3T3-L1 | IC50 |
28 nM
Compound: 59
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Inhibition of forskolin-stimulated lipolysis in differentiated 3T3-L1 cells
Inhibition of forskolin-stimulated lipolysis in differentiated 3T3-L1 cells
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[PMID: 15149665] |
BAY 59-9435 significantly diminishes the isoproterenol-increased media IL-6. BAY 59-9435 blocks isoproterenol-induced SphK1 expression[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:3T3-L1 cells.
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Concentration:10 μM.
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Incubation Time:1 h.
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Result:Pretreatment with BAY completely abrogated the induction of SphK1 expression by CL.
Chemical Information
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CAS No. 654059-21-9
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Appearance Solid
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Masse moléculaire 266.34
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Formule C14H22N2O3
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Color White to off-white
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SMILES
O=C1C(C(C)C)=C(C)N(C(N2C[C@@H](C)CCC2)=O)O1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (6)
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Journal Impact Factor
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Most Recent
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Cell Metab
Single-nucleus transcriptomics identifies separate classes of UCP1 and futile cycle adipocytes. [Abstract]2024 Sep 3;36(9):2130-2145.e7. PMID: 39084216 -
Cell Metab
Lipid-laden lung mesenchymal cells foster breast cancer metastasis via metabolic reprogramming of tumor cells and natural killer cells. [Abstract]2022 Dec 6;34(12):1960-1976.e9. PMID: 36476935 -
Nat Chem Biol
2025 Aug 5. PMID: 40764678 -
Microcirculation
Adipose Triglyceride Lipase and Gpr40 Contribute to the Anti-Contractile Effect of Perivascular Adipose Tissue. [Abstract]2026 May;33(4):e70063. PMID: 42076883 -
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Solvant et solubilité
DMSO : 100 mg/mL (375.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 5 mg/mL (18.77 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 5 mg/mL (18.77 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Ebdrup S, et al. Synthesis and structure-activity relationship for a novel class of potent and selective carbamoyl-triazole based inhibitors of hormone sensitive lipase. J Med Chem. 2004 Jan 15;47(2):400-10. [Content Brief]
[2]. Elizabeth A Rondini, et al. Novel Pharmacological Probes Reveal ABHD5 as a Locus of Lipolysis Control in White and Brown AdipocytesJ Pharmacol Exp Ther. 2017 Dec;363(3):367-376. [Content Brief]
[3]. Wenliang Zhang, et al. Adipocyte lipolysis-stimulated interleukin-6 production requires sphingosine kinase 1 activity. J Biol Chem. 2014 Nov 14;289(46):32178-85. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7546 mL | 18.7729 mL | 37.5459 mL | 93.8646 mL |
| 5 mM | 0.7509 mL | 3.7546 mL | 7.5092 mL | 18.7729 mL | |
| 10 mM | 0.3755 mL | 1.8773 mL | 3.7546 mL | 9.3865 mL | |
| 15 mM | 0.2503 mL | 1.2515 mL | 2.5031 mL | 6.2576 mL | |
| 20 mM | 0.1877 mL | 0.9386 mL | 1.8773 mL | 4.6932 mL | |
| 25 mM | 0.1502 mL | 0.7509 mL | 1.5018 mL | 3.7546 mL | |
| 30 mM | 0.1252 mL | 0.6258 mL | 1.2515 mL | 3.1288 mL | |
| 40 mM | 0.0939 mL | 0.4693 mL | 0.9386 mL | 2.3466 mL | |
| 50 mM | 0.0751 mL | 0.3755 mL | 0.7509 mL | 1.8773 mL | |
| 60 mM | 0.0626 mL | 0.3129 mL | 0.6258 mL | 1.5644 mL | |
| 80 mM | 0.0469 mL | 0.2347 mL | 0.4693 mL | 1.1733 mL | |
| 100 mM | 0.0375 mL | 0.1877 mL | 0.3755 mL | 0.9386 mL |
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