ddCTP trilithium
ddCTP trilithium is a type of chain-terminating deoxynucleotide. ddCTP trilithium can be incorporated into the extension primer chain that lacks the 3'-hydroxyl group, thereby terminating primer extension, viral genome replication, and DNA synthesis. ddCTP trilithium can distinguish almost identical RNA through distinguishable extension products in primer extension inhibition experiments. ddCTP trilithium is the active metabolite of Zalcitabine (HY-17392), which can competitively inhibit HIV reverse transcriptase, terminate the synthesis of viral DNA chains, and thereby inhibit HIV replication.
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- CAS No.: 93939-77-6
- Formule: C9H13Li3N3O12P3
- Masse moléculaire:468.96
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Chemical Information
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Activité biologique
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DNA Polymerase |
ddCTP (0.1 mM; 20 min) trilithium enables discrimination of nearly identical RNA sequences via chain termination during poisoned primer extension reactions using total cellular RNA or purified in vitro transcripts, generating extension products of distinct sizes detectable by gel electrophoresis[1].
In addition to inhibiting viral reverse transcriptase, ddCTP trilithium may also inhibit DNA polymerase γ in mammalian cells. This enzyme is responsible for mitochondrial DNA synthesis. Inhibition of DNA polymerase γ leads to decreased mitochondrial DNA content and impaired function[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 93939-77-6
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Masse moléculaire 468.96
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Formule C9H13Li3N3O12P3
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SMILES
O=[P](O[P](O[P](O)(O[Li])=O)(O[Li])=O)(O[Li])OC[C@@H]1CC[C@H](N2C(N=C(N)C=C2)=O)O1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
[1].
Nilsen TW. Poisoned primer extension. Cold Spring Harb Protoc. 2015 Jan 5;2015(1):pdb.prot080986.
[Content Brief]
[3].
Z G Chidgeavadze, et al. 3'-Fluoro-2',3'-dideoxyribonucleoside 5'-triphosphates: terminators of DNA synthesis. FEBS Lett. 1985 Apr 22;183(2):275-8.
[Content Brief]
[4]. Z G Chidgeavadze, et al. 3'-Fluoro-2',3'-dideoxyribonucleoside 5'-triphosphates: terminators of DNA synthesis. FEBS Lett. 1985 Apr 22;183(2):275-8. [Content Brief]
[5]. Pelletier H, et al. Structures of ternary complexes of rat DNA polymerase beta, a DNA template-primer, and ddCTP. Science. 1994 Jun 24;264(5167):1891-903. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
ddCTP trilithium Related Classifications
HY-137697B Related Classifications
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- ddCTP trilithium
- 93939-77-6
- Nucleoside Antimetabolite/Analog
- HIV
- Drug Metabolite
- DNA/RNA Synthesis
- HIV Protease
- RNAs
- total cellular RNA
- rat DNA polymerase β
- purified in vitro transcript
- viral genome replication
- DNA synthesis
- human HIV-1 reverse transcriptase
- RNA sequences
- poisoned primer extension assays
- DNA template-primer
- Inhibitor
- inhibitor
- inhibit