ddCTP 

ddCTP is a type of chain-terminating deoxynucleotide. ddCTP can be incorporated into the extension primer chain that lacks the 3'-hydroxyl group, thereby terminating primer extension, viral genome replication, and DNA synthesis. ddCTP can distinguish almost identical RNA through distinguishable extension products in primer extension inhibition experiments. ddCTP is the active metabolite of Zalcitabine (HY-17392), which can competitively inhibit HIV reverse transcriptase, terminate the synthesis of viral DNA chains, and thereby inhibit HIV replication^[1][2][3].

ddCTP (0.1 mM; 20 min) enables discrimination of nearly identical RNA sequences via chain termination during poisoned primer extension reactions using total cellular RNA or purified in vitro transcripts, generating extension products of distinct sizes detectable by gel electrophoresis^[1].
ddCTP, in addition to inhibiting viral reverse transcriptase, may also inhibit DNA polymerase γ in mammalian cells. This enzyme is responsible for mitochondrial DNA synthesis. Inhibition of DNA polymerase γ leads to decreased mitochondrial DNA content and impaired function^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

451.16

C₉H₁₆N₃O₁₂P₃

66004-77-1

O=[P](O[P](O[P](O)(O)=O)(O)=O)(O)OC[C@@H]1CC[C@H](N2C(N=C(N)C=C2)=O)O1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nilsen TW. Poisoned primer extension. Cold Spring Harb Protoc. 2015 Jan 5;2015(1):pdb.prot080986.
  

  [2]. Adkins JC, et al. An update of its pharmacodynamic and pharmacokinetic properties and clinical efficacy in the management of HIV infection. Drugs. 1997 Jun;53(6):1054-80.
  

  [3]. Z G Chidgeavadze, et al. 3'-Fluoro-2',3'-dideoxyribonucleoside 5'-triphosphates: terminators of DNA synthesis. FEBS Lett. 1985 Apr 22;183(2):275-8.
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