Art. -Nr.: HY-N0468

Rebaudioside D

1 Cited Publications

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Rebaudioside D is an orally active sweetener that targets and activates FXR, modulates Acetyl-CoA Carboxylase, and inhibits 3-hydroxy-3-methylglutaryl-CoA reductase. Rebaudioside D regulates bile acid homeostasis and lipid metabolism, reduces the synthesis rates of fatty acids and cholesterol, and exerts multiple effects including anti-adipogenesis, hepatoprotection, anti-steatosis, gut microbiota modulation, enhancement of secondary bile acid metabolism, anti-endotoxin activity, regulation of bile acid transport, and inhibition of bile acid efflux. Rebaudioside D also reduces body weight gain, visceral fat accumulation, hepatic triglyceride and cholesterol accumulation, hepatic lipid peroxidation, and decreases the circulating level of lipopolysaccharide-binding protein. Rebaudioside D additionally enhances the secondary bile acid metabolic pathway of intestinal bacteria, upregulates the gene expression of ileal organic solute transporter α, and downregulates the gene expression of hepatic bile salt export pump. Rebaudioside D does not affect glucose homeostasis, alter total caloric intake or fecal energy excretion, induce weight gain, exacerbate obesity, promote hepatic steatosis, impair brown adipose tissue function, nor change skeletal muscle metabolism-related proteins. Rebaudioside D can be used in diet-induced obesity and obesity-related research.

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Reinheit: 98.48%
CAS. Nr.: 63279-13-0
Formel: C₅₀H₈₀O₂₈
Molecular Weight:1129.15

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Rebaudioside D Chemical Structure

CAS. Nr.: 63279-13-0

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Biologische Aktivität
- Beschreibung
- In Vivo
Chemical Information
Publications (1)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

In Vivo

Rebaudioside D (50 mg/kg; p.o.; daily; 12 weeks) reduces obesity in mice with obesity induced by a high-fat high-sugar (HFHS) diet, alleviates hepatic steatosis and lipid peroxidation, decreases circulating LBP levels, and increases the abundance of Faecalibaculum rodentium in the gut microbiota, without impairing glucose homeostasis^[1].
Rebaudioside D (0.2 g/L (0.02%); ad libitum access via drinking water; continuous administration for 16 weeks) does not induce obesity, hepatic steatosis or brown adipose tissue dysfunction, nor does it interfere with skeletal muscle metabolism in mice fed a regular diet^[1].
Rebaudioside D (0.2 g/L (0.02%); free access via drinking water; ad libitum access; for 16 consecutive weeks) does not exacerbate obesity or hepatic steatosis in male mice fed a high-fat diet. It maintains the expression of thermogenic proteins in brown adipose tissue, reduces several serum lipid and blood glucose parameters, and shifts the gut microbiota toward a more beneficial profile^[2].
Rebaudioside D (0.2 g/L (0.02%); ad libitum access via drinking water for 16 weeks) does not increase the expression of hepatic lipogenic proteins in female mice fed a high-fat diet^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57BL/6 J (8-week-old male, high-fat/high-sucrose diet-induced obesity)^[1]
Dosage:

50 mg/kg
Administration:

p.o.; daily; 12 weeks
Result:

Reduced weight gain significantly at weeks 8 and 10 through 12 compared to HFHS-fed vehicle controls.
Decreased the sum of all white adipose tissues, with a specific reduction in epididymal adipose tissue weight.
Reduced liver triglyceride and cholesterol levels, and decreased hepatic lipid peroxidation (measured by TBARS).

Animal Model:

C57BL/6J (male, 8-10 weeks old)^[2]
Dosage:

0.2 g/L (0.02%)
Administration:

in drinking water; ad libitum; daily for 16 weeks
Result:

Showed no significant difference in oral glucose tolerance test AUC, with a non-significant decreasing trend in fasting blood glucose levels compared to HFD controls.
Had a relative abundance of Bacillota phylum of 75.3%, relative abundance of Akkermansia of 8.07%, and relative abundance of Faecalibaculum of 13.57%.
Identified short-chain fatty acid-producing genera including Faecalibaculum, Acetatifactor, and Parabacteroides as biomarkers via LEfSe analysis.

Chemical Information

CAS. Nr. 63279-13-0
Appearance Solid
Molecular Weight 1129.15
Formel C₅₀H₈₀O₂₈
Color White to off-white
SMILES

O[C@H]1[C@H](O)[C@@H](CO)O[C@@H](OC([C@@]2(C)[C@](CC[C@]3(CC4=C)[C@@]5([H])CC[C@]4(O[C@]6([H])O[C@H](CO)[C@@H](O)[C@H](O[C@]7([H])O[C@H](CO)[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O[C@@]8([H])[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O8)C3)([H])[C@@]5(C)CCC2)=O)[C@@H]1O[C@@]9([H])[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O9
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
- Plants
- other families
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Mol Catal

2025 Nov 2;588:115566.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (88.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

0.8856 mL

4.4281 mL

8.8562 mL

5 mM

0.1771 mL

0.8856 mL

1.7712 mL

10 mM

0.0886 mL

0.4428 mL

0.8856 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (2.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (2.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (2.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (285 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Morissette A, et al. Rebaudioside D decreases adiposity and hepatic lipid accumulation in a mouse model of obesity. Sci Rep. 2024;14(1):3077. Published 2024 Feb 6. [Content Brief]

[2]. Lim S, et al. Rebaudioside D and M, the next-generation sugar substitutes, do not exacerbate metabolic dysfunction in high-fat diet mice. Food Chem X. 2025;29:102679. Published 2025 Jun 19. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	0.8856 mL	4.4281 mL	8.8562 mL	22.1405 mL
	5 mM	0.1771 mL	0.8856 mL	1.7712 mL	4.4281 mL
	10 mM	0.0886 mL	0.4428 mL	0.8856 mL	2.2141 mL
	15 mM	0.0590 mL	0.2952 mL	0.5904 mL	1.4760 mL
	20 mM	0.0443 mL	0.2214 mL	0.4428 mL	1.1070 mL
	25 mM	0.0354 mL	0.1771 mL	0.3542 mL	0.8856 mL
	30 mM	0.0295 mL	0.1476 mL	0.2952 mL	0.7380 mL
	40 mM	0.0221 mL	0.1107 mL	0.2214 mL	0.5535 mL
	50 mM	0.0177 mL	0.0886 mL	0.1771 mL	0.4428 mL
	60 mM	0.0148 mL	0.0738 mL	0.1476 mL	0.3690 mL
	80 mM	0.0111 mL	0.0554 mL	0.1107 mL	0.2768 mL

Keywords

Rebaudioside D

1 Cited Publications

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Publications Citing Use of MedChemExpress (MCE) Rebaudioside D

Biologische Aktivität

Chemical Information

Publications (1)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Rebaudioside D Related Classifications

Art. -Nr.: HY-N0468