Cat. No.: HY-112083

BAY-3827

18 Cited Publications

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Based on 18 publication(s) in Google Scholar

BAY-3827, a chemical probe, is a potent and selective AMPK inhibitor with IC₅₀ values of 1.4 nM at low (10 μM ATP concentration) and 15 nM at high (2 mM ATP concentration). BAY-3827 shows over 500-fold selectivity for most of the 331 kinases. BAY-3827 prevents phosphorylation of acetyl-CoA carboxylase 1 and shows strongest anti-proliferative activity in androgen-dependent prostate cancer cell lines.

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Pureté: 99.72%
CAS No.: 2377576-35-5
Formule: C₂₇H₂₅FN₆O
Masse moléculaire:468.53

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

BAY-3827 Chemical Structure

CAS No.: 2377576-35-5

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (18)
Solvant et solubilité
- In Vitro
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

IC50: 1.4 nM (AMPK kinase,10 µM ATP), 15 nM (AMPK kinase, 2 mM ATP), 1324 nM (Aurora A), 124 nM (Flt3), 788 nM (c-Met), 36 nM (Rsk4)^[1]

Voir tous les produits spécifiques à Isoform AMPK :

In Vitro

BAY-3827 (0-200 μM) inhibits AMPK kinase activity with IC₅₀ values of 1.4 nM at low, 10 μM ATP concentration and 15 nM at high, 2 mM ATP concentration^[1].
BAY-3827 (0-200 μM) inhibits Aurora A, Flt3, c-Met and Rsk4 with IC₅₀ values of 1324, 124, 788 and 36 nM , respectively with 10 μM ATP concentration^[1].
BAY-3827 (overnight) strongly reduces ACC1 Ser79 phosphorylation in LNCaP and VCaP cells, and shows a lesser extent in IMR-32 and especially in Colo320 cells^[1].
BAY-3827 (0-10 nM; 6 d) shows strong inhibitory effects to LNCaP and VCaP cells^[1].
BAY-3827 (1 and 5 μM; 24 and 48 h) represses LIPE gene expression, reduces serine/threonine kinase AKT3 and blocks the expression of several genes from the mitochondrial carnitine palmitoyltransferase (CPT) family which is involved in acyl carnitine formation in VCaP cells^[1].
BAY-3827 (5 μM; 2-4 d) significantly increases the formation of lipid droplets in comparison to androgen treatment only^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

LNCaP, VCaP, 22Rv1, C4-2B, PC-3 and DU-145 prostate cancer cell lines
Concentration:

0-10 nM
Incubation Time:

6 d
Result:

Showed strong inhibitory effects for LNCaP and VCaP cells, two prostate cancer cell lines with IC₅₀ values of 0.28 and 1.71 nM, respectily. Inhibited proliferation of 22Rv1 cells with an IC₅₀ value of 5.55 nM.

Chemical Information

CAS No. 2377576-35-5
Appearance Solid
Masse moléculaire 468.53
Formule C₂₇H₂₅FN₆O
Color White to off-white
SMILES

CCC1=CC=CC=C1C(NC2=NNC3=C2C=C(C4C(C#N)=C(C)N(C)C(C)=C4C#N)C(F)=C3C)=O
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (18)

All In Vivo Efficacy StudyWBHistological Imaging/StainingRT-PCRELISA

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Journal Impact Factor

Bioact Mater

Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury. [Abstract]

2026 Apr 9:63:373-389. PMID: 42006003
J Extracell Vesicles

Stromal cell-derived small extracellular vesicles enhance radioresistance of prostate cancer cells via interleukin-8-induced autophagy. [Abstract]

2023 Jul;12(7):e12342. PMID: 37387557
Autophagy

AMPK promotes TFEB transcriptional activity through dephosphorylation at both MTORC1-dependent and -independent sites. [Abstract]

2026 Feb 9. PMID: 41661247
Nat Commun

Attenuated growth factor signaling during cell death initiation sensitizes membranes towards peroxidation. [Abstract]

2025 Feb 25;16(1):1774. PMID: 40000627
Phytomedicine

Exploring the therapeutic potential of HAPC in COVID-19-induced acute lung injury. [Abstract]

2025 Apr:139:156563. PMID: 40023068
J Pharm Anal

2025 Dec 15.

BAY-3827 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Dec 15.

BAY-3827 at 10 mg/kg for a duration of 4 weeks. Distinctive micro-CT visuals showcasing both longitudinal and cross-sectional views of the distal femur segments.

BAY-3827 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Dec 15.

BAY-3827 was administered at 10 mg/kg for a duration of 4 weeks. Representative images of hematoxylin and eosin (H&E) staining and Masson's staining of the metaphyseal tissue sections of the thigh were presented.
Cell Chem Biol

Genetically encoded manipulation of ATP/ADP ratio in human cells uncovers proteomic and physiological signatures of energy stress. [Abstract]

2026 May 21;33(5):683-698.e7. PMID: 41980778
J Agric Food Chem

Natural Dietary Flavonoid Apigenin Mitigates Ulcerative Colitis via Modulating the AMPK/NF-κB/NLRP3 Signaling Axis. [Abstract]

2025 Dec 22. PMID: 41428381

BAY-3827 purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2025 Dec 22. [Abstract]

ELISA quantification of IL-1β in BMDM supernatants following LPS + nigericin stimulation with or without Api, the AMPK agonist AICAR, or the AMPK inhibitor BAY-3827 (500 nM) was performed.
Commun Biol

Dorsomorphin inhibits AMPK, upregulates Wnt and Foxo genes and promotes the activation of dormant follicles. [Abstract]

2024 Jun 20;7(1):747. PMID: 38902324
Cancer Biol Ther

Cooperative CCL2/CCR2 and HGF/MET signaling enhances breast cancer growth and invasion associated with metabolic reprogramming. [Abstract]

2025 Dec;26(1):2535824. PMID: 40736024
Int J Mol Sci

Comparison of the Effects of Sodium-Glucose Cotransporter 2 Inhibitors on Cardiac Fibroblast Properties. [Abstract]

2025 Oct 16;26(20):10098. PMID: 41155391

BAY-3827 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Oct 16;26(20):10098. [Abstract]

HCFs were incubated with the AMPK inhibitor BAY-3827 (500 nM) for 1 h prior to stimulation with DMSO (vehicle) or indicated concentrations of SGLT2i for 3 h.

BAY-3827 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Oct 16;26(20):10098. [Abstract]

HCFs were incubated with the AMPK inhibitor BAY-3827 (500 nM) for 1 h prior to stimulation with DMSO (vehicle) or indicated concentrations of SGLT2i for 72 h.
Int Immunopharmacol

Anti-Na+/K+-ATPase DR antibody attenuates UUO-induced renal fibrosis through inhibition of Na+/K+-ATPase α1-dependent HMGB1 release. [Abstract]

2023 Mar:116:109826. PMID: 36764269
Dis Model Mech

C-C chemokine ligand 2 signaling promotes skeletal muscle wasting in non-tumor and breast tumor mouse models. [Abstract]

2024 Jul 8:dmm.050398. PMID: 38973385
Toxicol In Vitro

Isobavachin induces autophagy-mediated cytotoxicity in AML12 cells via AMPK and PI3K/Akt/mTOR pathways. [Abstract]

2024 Aug 16:105919. PMID: 39154867
bioRxiv

2026 May 21.
bioRxiv

2025 March 03.
bioRxiv

Adiponectin Signaling Regulates Urinary Bladder Function by Blunting Smooth Muscle Purinergic Contractility. [Abstract]

2024 Oct 29:2024.10.25.620328. PMID: 39554160
bioRxiv

2024 October 25.

Solvant et solubilité

In Vitro:

DMSO : 25 mg/mL (53.36 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1343 mL

10.6717 mL

21.3434 mL

5 mM

0.4269 mL

2.1343 mL

4.2687 mL

10 mM

0.2134 mL

1.0672 mL

2.1343 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Lemos C, et al. The potent AMPK inhibitor BAY-3827 shows strong efficacy in androgen-dependent prostate cancer models. Cell Oncol (Dordr). 2021 Jun;44(3):581-594. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1343 mL	10.6717 mL	21.3434 mL	53.3584 mL
	5 mM	0.4269 mL	2.1343 mL	4.2687 mL	10.6717 mL
	10 mM	0.2134 mL	1.0672 mL	2.1343 mL	5.3358 mL
	15 mM	0.1423 mL	0.7114 mL	1.4229 mL	3.5572 mL
	20 mM	0.1067 mL	0.5336 mL	1.0672 mL	2.6679 mL
	25 mM	0.0854 mL	0.4269 mL	0.8537 mL	2.1343 mL
	30 mM	0.0711 mL	0.3557 mL	0.7114 mL	1.7786 mL
	40 mM	0.0534 mL	0.2668 mL	0.5336 mL	1.3340 mL
	50 mM	0.0427 mL	0.2134 mL	0.4269 mL	1.0672 mL

Keywords

BAY-3827

18 Cited Publications

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BAY-3827

Epigenetics Compound Library

Kinase Inhibitor Library

PI3K/Akt/mTOR Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Lipid Metabolism Compound Library

Glucose Metabolism Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Highly Selective Inhibitors Library

Serine/Threonine Kinase Inhibitor Library

Anti-Ovarian Cancer Compound Library

Radioprotector Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

Epigenetics Compound Library Mini

Kinase Inhibitor Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) BAY-3827

Voir tous les produits spécifiques à Isoform AMPK

Activité biologique

Chemical Information

Publications (18)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

BAY-3827 Related Classifications

HY-112083 Related Classifications