GAPDH-IN-1
Based on 1 Customer Validation
GAPDH-IN-1 (Compound F8) is a GAPDH inhibitor (IC50 of 39.31 μM for GAPDH enzymatic activity). GAPDH-IN-1 forms a covalent adduct with an aspartic acid in the active site to displace NAD+, a cofactor of the enzyme, with concomitant enhancement of the cysteine-reactive probe reaction with the catalytic cysteine.
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- Pureté: 98.10%
- CAS No.: 252212-58-1
- Formule: C20H18N2O5
- Masse moléculaire:366.37
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Activité biologique
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Chemical Information
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Activité biologique
GAPDH-IN-1 (1.6-50 μM) exhibits a dose-dependent increase in the probe labeling of the 40 kDa protein[1].
GAPDH-IN-1 (50 μM, 4 h) covalently reacts with Asp35 but not cysteine residues in GAPDH, stimulating the subsequent reaction of Cys152 with the iodoacetamide-based (IA) probe[1].
GAPDH-IN-1 (25-50 μM, 24 h) enhances probe and GAPDH responses less in HEK293 cells than in cell lysates[1].
GAPDH-IN-1 (0-100 μM, 24 h) displays a concentration-dependent inactivation of GAPDH in HEK293 cell lysates, with an IC50 of 39.31 μM[1].
GAPDH-IN-1 (0-100 μM) inhibits the viability of HEK293 cells, with an IC50 of 50.64 μM[1].
GAPDH-IN-1-GAPDH adduct can be reduced by the addition of excess NAD+, as evidenced by the band shifts of endogenous GAPDH in a silver-stained gel[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293 cells transiently expressing FLAG-tagged GAPDH or its cysteine-to-alanine mutants
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Concentration:50 μM
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Incubation Time:4 h
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Result:The band of FLAG-GAPDH was slightly shifted.
Showed band shifts in all the cysteine mutants (C152A, C156A, and C247A).
Chemical Information
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CAS No. 252212-58-1
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Appearance Solid
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Masse moléculaire 366.37
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Formule C20H18N2O5
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Color White to off-white
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SMILES
C1(C2=CC(OCC3OC3)=CC=C2)=NN=C(C4=CC(OCC5OC5)=CC=C4)O1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Pureté et documentation
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Fiche technique (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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