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Cat. No.: HY-D0159

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ZnAF-1F

ZnAF-1F is a potent fluorophore for with an K_d value of 2.2 nM. ZnAF-1F can be used as fluorescent probes for Zn²⁺ in cells. ZnAF-1F shows λ excitation of 489 nm and λ emission of 514 nm.

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CAS No.: 443302-08-7
Formule: C₃₄H₂₆F₂N₄O₅
Masse moléculaire:608.59

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-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

ZnAF-1F Chemical Structure

CAS No.: 443302-08-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

ZnAF-1F is a potent fluorophore for with an K_d value of 2.2 nM. ZnAF-1F can be used as fluorescent probes for Zn²⁺ in cells. ZnAF-1F shows λ excitation of 489 nm and λ emission of 514 nm^[1]^[2]^[3].

In Vitro

ZnAF-1F (1 μM) shows an K_on value of 3.5*10⁶ M^-1s^-1, and an K_off value of 7.7*10^-3 s^-1 in 100mM HEPES buffer^[1].
ZnAF-1F is a Zn sensors in neutral and slightly acidic conditions^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

514

Excitation (Ex)

489

Chemical Information

CAS No. 443302-08-7
Appearance Solid
Masse moléculaire 608.59
Formule C₃₄H₂₆F₂N₄O₅
Color Orange to red
SMILES

O=C1OC2(C3=C(OC4=C2C=C(F)C(O)=C4)C=C(O)C(F)=C3)C5=C1C=C(NCCN(CC6=NC=CC=C6)CC7=NC=CC=C7)C=C5
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (275 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Hirano T, et al. Improvement and biological applications of fluorescent probes for zinc, ZnAFs. J Am Chem Soc. 2002 Jun 12;124(23):6555-62. [Content Brief]

[2]. Zhaohua Dai, et al. Tailoring tripodal ligands for zinc sensing. New J. Chem., 2007,31, 1708-1718.

[3]. Que EL, et al. Metals in neurobiology: probing their chemistry and biology with molecular imaging. Chem Rev. 2008 May;108(5):1517-49. [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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MOQ

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100 mg

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