1. Apoptosis
  2. Apoptosis
  3. CMLD-2

CMLD-2 

Cat. No.: HY-124828 Purity: 98.59%
Handling Instructions

CMLD-2, an inhibitor of HuR-ARE interaction, competitively binds HuR protein disrupting its interaction with adenine-uridine rich elements (ARE)-containing mRNAs (Ki=350 nM). CMLD-2 induces apoptosis exhibits antitumor activity in different cancer cells as colon, pancreatic, thyroid and lung cancer cell lines. Hu antigen R (HuR) is an RNA binding protein, can regulate target mRNAs stability and translation.

For research use only. We do not sell to patients.

CMLD-2 Chemical Structure

CMLD-2 Chemical Structure

CAS No. : 958843-91-9

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5 mg USD 300 In-stock
Estimated Time of Arrival: December 31
10 mg USD 500 In-stock
Estimated Time of Arrival: December 31
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Description

CMLD-2, an inhibitor of HuR-ARE interaction, competitively binds HuR protein disrupting its interaction with adenine-uridine rich elements (ARE)-containing mRNAs (Ki=350 nM). CMLD-2 induces apoptosis exhibits antitumor activity in different cancer cells as colon, pancreatic, thyroid and lung cancer cell lines. Hu antigen R (HuR) is an RNA binding protein, can regulate target mRNAs stability and translation[1][2].

IC50 & Target

HuR[1]

In Vitro

CMLD-2 (1-75 μM; 24-72 h) inhibits thyroid cancer cell viability[2].
CMLD-2 (20-30 μM; 24-48 h) activates caspases and induces apoptotic cell death in H1299 and A549 cells[3].
CMLD-2 (30 μM; 24-48 h) induces G1 cell cycle arrest and mitochondrial perturbation in H1299 and A549 cell[3].
CMLD-2 (30 μM; 24-48 h) reduces expression of HuR and HuR-regulated mRNAs and proteins in H1299 cells[3].
CMLD-2 (35 μM; 72 h) decreases directional migration capability in SW1736, 8505C, BCPAP and K1 cells. CMLD-2 induces a strong decrease of MAD2 mRNA levels in SW1736, 8505C, BCPAP and K1 cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: SW1736, 8505C, BCPAP and K1 cells
Concentration: 1, 5, 10, 25, 35, 50, 75 μM
Incubation Time: 24, 48, 72 hours
Result: Reduced the viability of all the four cell lines when used at 35, 50 and 75 μM concentration and at different time points.

Apoptosis Analysis[3]

Cell Line: H1299, A549, H1975, HCC827, MRC-9 and CCD16 cells
Concentration: 20, 30 μM
Incubation Time: 24, 48 hours
Result: Marked activated the caspase-9 and -3 in lung tumor cells.
Induce the cleavage of PARP in lung tumor cells.
Significantly increased the annexin-V-positive staining in lung tumor cells.

Cell Cycle Analysis[3]

Cell Line: H1299, A549, MRC-9 and CCD16 cells
Concentration: 30 μM
Incubation Time: 24, 48 hours
Result: Induced greater G1 phase cell cycle arrest in H1299 and A549 cells than in MRC-9 and CCD16 cells.

Western Blot Analysis[3]

Cell Line: H1299, A549, H1975, HCC827, CCD16 and MRC-9 cells
Concentration: 20, 30 μM
Incubation Time: 24, 48 hours
Result: Diminished protein expression of HuR, Bcl-2, Cyclin E and Bcl-XL and increased expression of p27 and BAX in lung tumor cells.
Molecular Weight

513.58

Formula

C₃₁H₃₁NO₆

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (97.36 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9471 mL 9.7356 mL 19.4712 mL
5 mM 0.3894 mL 1.9471 mL 3.8942 mL
10 mM 0.1947 mL 0.9736 mL 1.9471 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.87 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.87 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.87 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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CMLD-2
Cat. No.:
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