Cat. No.: HY-13764A

dl-Tetrandrine

dl-Tetrandrine is an orally active and brain-penetrant calcium channel blocker that inhibits voltage-dependent calcium channels. dl-Tetrandrine selectively blocks Ca²⁺ influx with an IC₅₀ value of approximately 1-10 μM. dl-Tetrandrine exerts anti-inflammatory, immunosuppressive, and anti-arrhythmic activities by inhibiting intracellular calcium overload, and can reverse multidrug resistance in tumor cells. dl-Tetrandrine is promising for research of autoimmune diseases (such as rheumatoid arthritis), cardiovascular diseases, and tumor drug resistance reversal.

For research use only. We do not sell to patients.

CAS No.: 23495-89-8
Formula: C₃₈H₄₂N₂O₆
Molecular Weight:622.75

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (276 KB)

dl-Tetrandrine Chemical Structure

CAS No.: 23495-89-8

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Biological Activity
- Description
- Cellular Effect
Chemical Information
Purity & Documentation
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Biological Activity

Description

All Calcium Channel Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A673	GI₅₀	4 μM Compound: 16	Antiproliferative activity against human A673 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human A673 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
CCRF-CEM	IC₅₀	15.8 μM Compound: 1	Antiproliferative activity against vincristine-resistant human CEM cells assessed as reduction in cell viability measured after 48 hrs by Celltiter-blue cell viability assay Antiproliferative activity against vincristine-resistant human CEM cells assessed as reduction in cell viability measured after 48 hrs by Celltiter-blue cell viability assay	[PMID: 32942071]
ECa-109 cell line	IC₅₀	29.37 nM Compound: TET	Reversal of VCR -resistance in human Eca-109 cells assessed as vincristine IC50 by measuring cell viability incubated for 48 hrs by CCK-8 method (Rvb = 6830.0+/-537.0 nM) Reversal of VCR -resistance in human Eca-109 cells assessed as vincristine IC50 by measuring cell viability incubated for 48 hrs by CCK-8 method (Rvb = 6830.0+/-537.0 nM)	[PMID: 36892076]
ECa-109 cell line	IC₅₀	29.4 nM Compound: TET	Reversal of VCR -resistance in human Eca-109 cells assessed as cell viability at 5 uM incubated for 48 hrs by CCK-8 method (Rvb = 6830.0+/-537.0 nM) Reversal of VCR -resistance in human Eca-109 cells assessed as cell viability at 5 uM incubated for 48 hrs by CCK-8 method (Rvb = 6830.0+/-537.0 nM)	[PMID: 36892076]
HCC1806	GI₅₀	5.1 μM Compound: 16	Antiproliferative activity against human HCC1806 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human HCC1806 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
HCC1937	GI₅₀	4.2 μM Compound: 16	Antiproliferative activity against human HCC1937 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human HCC1937 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
HCC70	GI₅₀	3.4 μM Compound: 16	Antiproliferative activity against human HCC70 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human HCC70 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
HEK-293T	CC₅₀	>1 μM Compound: 1; tet	Cytotoxicity against HEK293T cells assessed as reduction in cell viability by CellTiter-Glo assay Cytotoxicity against HEK293T cells assessed as reduction in cell viability by CellTiter-Glo assay	[PMID: 37043739]
HEL	IC₅₀	16.78 μM Compound: Tetrandrine	Cytotoxicity against human HEL cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HEL cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29133049]
HEL	IC₅₀	19.74 μM Compound: Tetrandrine	Cytotoxicity against human HEL cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HEL cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
HEL	IC₅₀	7.45 μM Compound: Tet	Antiproliferative activity against HEL cells after 48 hrs by MTT assay Antiproliferative activity against HEL cells after 48 hrs by MTT assay	[PMID: 30109000]
HeLa	IC₅₀	2.16 μM Compound: Tetrandrine	Inhibition of Ebolavirus glycoprotein/matrix protein VP40 entry in human HeLa cells after 4.5 hrs beta-lactamase reporter assay Inhibition of Ebolavirus glycoprotein/matrix protein VP40 entry in human HeLa cells after 4.5 hrs beta-lactamase reporter assay	[PMID: 29624387]
HepG2	IC₅₀	6.82 μM Compound: Tetrandrine	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 28057423]
K562	IC₅₀	11.76 μM Compound: Tet	Antiproliferative activity against human K562 cells after 48 hrs by MTT assay Antiproliferative activity against human K562 cells after 48 hrs by MTT assay	[PMID: 30109000]
K562	IC₅₀	13.81 μM Compound: Tetrandrine	Cytotoxicity against human K562 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human K562 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29133049]
K562	IC₅₀	6.43 μM Compound: Tetrandrine	Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
KB	ED₅₀	5800 nM Compound: 16	Cytotoxicity against human KB cells after 72 hrs by SRB assay Cytotoxicity against human KB cells after 72 hrs by SRB assay	[PMID: 9917283]
KB-V1	ED₅₀	3.7 μg/mL Compound: 1	Cytotoxicity against human multidrug-resistant KBV1 cells Cytotoxicity against human multidrug-resistant KBV1 cells	[PMID: 8450318]
L02	IC₅₀	44.25 μM Compound: Tet	Cytotoxicity against human HL7702 cells after 48 hrs by MTT assay Cytotoxicity against human HL7702 cells after 48 hrs by MTT assay	[PMID: 30109000]
MCF7	IC₅₀	19.58 μM Compound: Tetrandrine	Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MCF-7 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
MCF7	IC₅₀	8.62 μM Compound: Tetrandrine	Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTT assay	[PMID: 28057423]
MDA-MB-231	GI₅₀	5 μM Compound: 16	Antiproliferative activity against human MDA-MB-231 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human MDA-MB-231 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
MDA-MB-231	IC₅₀	18.45 μM Compound: Tetrandrine	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31784186]
MDA-MB-231	IC₅₀	21.97 μM Compound: Tetrandrine	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29133049]
MDA-MB-231	IC₅₀	8.94 μM Compound: Tet	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay	[PMID: 30109000]
MDA-MB-453	GI₅₀	3.3 μM Compound: 16	Antiproliferative activity against human MDA-MB-453 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human MDA-MB-453 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
P388	ED₅₀	0.4 μg/mL Compound: 1	Cytotoxicity against mouse P388 cells Cytotoxicity against mouse P388 cells	[PMID: 8450318]
Panel NCI-60 (60 carcinoma cell lines)	GI₅₀	10 nM Compound: Fanchinin	Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program Growth inhibitory activity against human cancer cell line in the NCI's anticancer drug screening program	[PMID: 15743190]
PC-3	IC₅₀	5.86 μM Compound: Tet	Antiproliferative activity against human PC3 cells after 48 hrs by MTT assay Antiproliferative activity against human PC3 cells after 48 hrs by MTT assay	[PMID: 30109000]
PC-3	IC₅₀	7.87 μM Compound: Tetrandrine	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29133049]
PLC-PRF-5	IC₅₀	18.46 μM Compound: Tetrandrine	Cytotoxicity against human PLC/PRF/5 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human PLC/PRF/5 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 28109948]
SJRH30	GI₅₀	3 μM Compound: 16	Antiproliferative activity against human SJRH30 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human SJRH30 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
U-937	IC₅₀	>50 μM Compound: 16, NSC 77037	Cytotoxicity against human U937 cells after 24 hrs by alamar blue assay Cytotoxicity against human U937 cells after 24 hrs by alamar blue assay	[PMID: 22766217]
Vero	CC₅₀	14.92 μM Compound: Tetrandrine	Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr	10.1101/2020.03.20.999730
Vero	IC₅₀	3 μM Compound: Tetrandrine	Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr	10.1101/2020.03.20.999730
Vero C1008	CC₅₀	>33 μM Compound: Tetrandrine (Fanchinine)	Toxicity CC50 against VERO-E6 cells determined at 48 hours by high content imaging (same conditions as 2_LEY without exposure to 0.01 MOI SARS CoV-2 virus) Toxicity CC50 against VERO-E6 cells determined at 48 hours by high content imaging (same conditions as 2_LEY without exposure to 0.01 MOI SARS CoV-2 virus)	10.6019/CHEMBL4495565
Vero C1008	EC₅₀	1.1 μM Compound: Hanfangchin A	Determination of antiviral efficacy in high-content imaging assay in Vero E6 cells infected with SARS-CoV-2 (USA-WA1/2020 isolate) at MOI 0.75 after 24 hrs Determination of antiviral efficacy in high-content imaging assay in Vero E6 cells infected with SARS-CoV-2 (USA-WA1/2020 isolate) at MOI 0.75 after 24 hrs	10.1101/2020.04.16.044016
Vero C1008	IC₅₀	>5.01 × 10⁵M Compound: COVC-2820786116	Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of VERO-E6 cells after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of VERO-E6 cells after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging	10.6019/CHEMBL4651402
Vero C1008	IC₅₀	11.66 μM Compound: Tetrandrine (Fanchinine)	Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of VERO-E6 cells after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of VERO-E6 cells after 48 hours exposure to 0.01 MOI SARS CoV-2 virus by high content imaging	10.6019/CHEMBL4495565
VSMC	IC₅₀	1.48 μM Compound: Tetrandrine	Antivegetative activity against rat VSMC Antivegetative activity against rat VSMC	[PMID: 20708932]

Chemical Information

CAS No. 23495-89-8
Molecular Weight 622.75
Formula C₃₈H₄₂N₂O₆
SMILES

COC1=C2C3=C(C=C1OC)CCN(C)[C@@]3([H])CC4=CC=C(OC)C(OC5=CC=C(C[C@@]6([H])C7=CC(O2)=C(C=C7CCN6C)OC)C=C5)=C4
Structure Classification
- Alkaloids
- Alkaloid Dimers
Initial Source
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yupraphat T, et al. ALKALOIDE AUS DER THAILÄNDISCHEN MENISPERMACEEN–DROGE KRUNG KHA MAO[J]. Planta Medica, 1974, 25(04): 315-324.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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dl-Tetrandrine

All Calcium Channel Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

dl-Tetrandrine Related Classifications

HY-13764A Related Classifications