Cat. No.: HY-12146

ELOVL6-IN-2

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

ELOVL6-IN-2 is a potent, orally active and selective ELOVL6 inhibitor. ELOVL6-IN-2 inhibits mouse ELOVL6 activities, with an IC₅₀ value of 34 nM.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 1067647-43-1
Formula: C₂₈H₂₃F₆N₃O₄
Molecular Weight:579.49

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 2 years , -20°C, 1 year

ELOVL6-IN-2 Chemical Structure

CAS No.: 1067647-43-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vivo
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

ELOVL6-IN-2 is a potent, orally active and selective ELOVL6 inhibitor. ELOVL6-IN-2 inhibits mouse ELOVL6 activities, with an IC₅₀ value of 34 nM^[1].

IC₅₀ & Target

IC50: 34 nM (ELOVL6)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
COS-7	IC₅₀	>10 μM Compound: 37	Inhibition of human ELOVL1 expressed in african green monkey COS7 cells Inhibition of human ELOVL1 expressed in african green monkey COS7 cells	[PMID: 19388647]
COS-7	IC₅₀	>10 μM Compound: 37	Inhibition of human ELOVL2 expressed in african green monkey COS7 cells Inhibition of human ELOVL2 expressed in african green monkey COS7 cells	[PMID: 19388647]
COS-7	IC₅₀	>10 μM Compound: 37	Inhibition of human ELOVL5 expressed in african green monkey COS7 cells Inhibition of human ELOVL5 expressed in african green monkey COS7 cells	[PMID: 19388647]
COS-7	IC₅₀	194 nM Compound: 37	Inhibition of mouse ELOVL3 expressed in african green monkey COS7 cells Inhibition of mouse ELOVL3 expressed in african green monkey COS7 cells	[PMID: 19388647]
COS-7	IC₅₀	31 nM Compound: 37	Inhibition of mouse ELOVL6 expressed in african green monkey COS7 cells Inhibition of mouse ELOVL6 expressed in african green monkey COS7 cells	[PMID: 19388647]
COS-7	IC₅₀	337 nM Compound: 37	Inhibition of human ELOVL3 expressed in african green monkey COS7 cells assessed as stearoyl-CoA elongation Inhibition of human ELOVL3 expressed in african green monkey COS7 cells assessed as stearoyl-CoA elongation	[PMID: 19388647]
COS-7	IC₅₀	8.9 nM Compound: 37	Inhibition of human ELOVL6 expressed in african green monkey COS7 cells assessed as palmitoyl-CoA elongation Inhibition of human ELOVL6 expressed in african green monkey COS7 cells assessed as palmitoyl-CoA elongation	[PMID: 19388647]

In Vivo

ELOVL6-IN-2 (0.1~1mg/kg; p.o.; 2 hours) potently and dose-proportionally suppresses the elongation index in the liver^[1].
ELOVL6-IN-2 (10mg/kg; p.o.; 2 hours) demonstrates highly liver penetrable^[1].
ELOVL6-IN-2 (1mg/kg; p.o.; 2~24 hours) exhibits sustained plasma exposure^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/6J mice
Dosage:

0.1~1mg/kg
Administration:

P.o
Result:

Potently and dose-proportionally suppressed the elongation index in the liver.

Animal Model:

Male C57BL/6J mice
Dosage:

10 mg/kg
Administration:

P.o
Result:

Demonstrated highly liver penetrable.

Animal Model:

Male C57BL/6J mice
Dosage:

1 mg/kg
Administration:

P.o
Result:

Exhibited sustained plasma exposure.

Chemical Information

CAS No. 1067647-43-1
Appearance Solid
Molecular Weight 579.49
Formula C₂₈H₂₃F₆N₃O₄
Color White to off-white
SMILES

O=C1N(C2=CC=CC=C2)C3=C(C(CC(C)(C)C3)=O)C1(C4=C(C)NN(C5=CC=C(OC(F)(F)F)C=C5)C4=O)C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All Cell Proliferation/Viability AssayIFIn Vivo Efficacy Study

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Journal Impact Factor

Gastroenterology

Oncogenic Fatty Acid Metabolism Rewires Energy Supply Chain in Gastric Carcinogenesis. [Abstract]

2024 Jan 24:S0016-5085(24)00064-7. PMID: 38272100
Nat Commun

Targeting ELOVL6 to disrupt c-MYC driven lipid metabolism in pancreatic cancer enhances chemosensitivity. [Abstract]

2025 Feb 16;16(1):1694. PMID: 39956817

ELOVL6-IN-2 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 16;16(1):1694. [Abstract]

Proliferation assay of cells with ELOVL6 inhibited by ELOVL6-IN-2 (10 nM; 24 h) compared to non-treated control in PDAC cell lines; n = 3 independent experiments.

ELOVL6-IN-2 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 16;16(1):1694. [Abstract]

ELOVL6-IN-2 (10 nM; 48 h). Representative images of immunofluorescence staining for dextran-rhodamine B 10,000 Da (red) and DAPI (blue) on PDAC cell lines.

ELOVL6-IN-2 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 16;16(1):1694. [Abstract]

ELOVL6-IN-2 (10 nM; 48 h). Colony assay of ELOVL6-inhibited cells comparedtonon-treated control in Panc1 (pBH and pBH MYC); representative images from n=3 independent experiments.

ELOVL6-IN-2 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 16;16(1):1694. [Abstract]

Tumor growth in nudemice subcutaneously implanted with T3M4 either ELOVL6-inhibited or non-treated control cells; Abraxane or PBS was administered i.p. at a dose of 40 mg/kg twice per week when tumor volume reached 500 mm³; ELOVL6-IN-2 or methylcellulose was administered by oral gavage at a dose of 10 mg/kg in combination with chemotherapy; n = 10 animals per treatment.

ELOVL6-IN-2 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 16;16(1):1694. [Abstract]

ELOVL6-IN-2 (10 mg/kg; oral gavage; twice per week) in combination with Abraxane yielded a remarkably substantial survival-promoting effect and inhibited tumor recurrence.
Nat Chem Biol

ELOVL6 activity attenuation induces mutant KRAS degradation. [Abstract]

2025 Sep 15. PMID: 40954224
Sci Adv

N6-methyladenosine modification-tuned lipid metabolism controls skin immune homeostasis via regulating neutrophil chemotaxis. [Abstract]

2024 Oct 4;10(40):eadp5332. PMID: 39356764
Biochim Biophys Acta Mol Basis Dis

Inhibition of ELOVL6 activity impairs mitochondrial respiratory function and inhibits tumor progression in FGFR3-mutated bladder cancer cells. [Abstract]

2025 Aug 18;1871(8):168023. PMID: 40835210
bioRxiv

2024 November 11.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (172.57 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7257 mL

8.6283 mL

17.2566 mL

5 mM

0.3451 mL

1.7257 mL

3.4513 mL

10 mM

0.1726 mL

0.8628 mL

1.7257 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (4.31 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. akahashi T, et al. Synthesis and evaluation of a novel indoledione class of long chain fatty acid elongase 6 (ELOVL6) inhibitors. J Med Chem. 2009;52(10):3142-3145. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7257 mL	8.6283 mL	17.2566 mL	43.1414 mL
	5 mM	0.3451 mL	1.7257 mL	3.4513 mL	8.6283 mL
	10 mM	0.1726 mL	0.8628 mL	1.7257 mL	4.3141 mL
	15 mM	0.1150 mL	0.5752 mL	1.1504 mL	2.8761 mL
	20 mM	0.0863 mL	0.4314 mL	0.8628 mL	2.1571 mL
	25 mM	0.0690 mL	0.3451 mL	0.6903 mL	1.7257 mL
	30 mM	0.0575 mL	0.2876 mL	0.5752 mL	1.4380 mL
	40 mM	0.0431 mL	0.2157 mL	0.4314 mL	1.0785 mL
	50 mM	0.0345 mL	0.1726 mL	0.3451 mL	0.8628 mL
	60 mM	0.0288 mL	0.1438 mL	0.2876 mL	0.7190 mL
	80 mM	0.0216 mL	0.1079 mL	0.2157 mL	0.5393 mL
	100 mM	0.0173 mL	0.0863 mL	0.1726 mL	0.4314 mL

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ELOVL6-IN-2

Metabolism/Protease Compound Library

Orally Active Compound Library

Targeted Diversity Library

Metabolic Enzyme Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ELOVL6-IN-2

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

ELOVL6-IN-2

Metabolism/Protease Compound Library

Orally Active Compound Library

Targeted Diversity Library

Metabolic Enzyme Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ELOVL6-IN-2

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

ELOVL6-IN-2 Related Classifications

HY-12146 Related Classifications