dTRIM24
Based on 8 publication(s) in Google Scholar
dTRIM24 is a selective bifunctional degrader of TRIM24 based on PROTAC, consists of ligands for von Hippel-Lindau and TRIM24.
For research use only. We do not sell to patients.
- Purity: 99.89%
- CAS No.: 2170695-14-2
- Formula: C55H68N8O13S2
- Molecular Weight:1113.30
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) dTRIM24
More- Mol Cell. 2021 Apr 1;81(7):1411-1424.e7. [Abstract]
- Proc Natl Acad Sci U S A. 2025 Aug 19;122(33):e2507571122. [Abstract]
- J Transl Med. 2021 Dec 9;19(1):505. [Abstract]
- Cell Death Discov. 2025 Mar 17;11(1):108. [Abstract]
- Structure. 2024 Dec 5;32(12):2352-2363.e8. [Abstract]
- Mol Carcinog. 2023 Aug;62(8):1119-1135. [Abstract]
- bioRxiv. 2025 May 24.
- Université de Toulouse. 2022 Feb 18.
All PROTACs Isoforms
More
Biological Activity
TRIM24[2].
dTRIM24 is a degrader of TRIM24 bromodomain. Recruitment of the VHL E3 ubiquitin ligase by dTRIM24 elicits potent and selective degradation of TRIM24. The anti-proliferative consequences of chemical degradation versus inhibition of TRIM24 are assessed. Growth over time is determined for MOLM-13 cells treated with dTRIM24, IACS-9571, VL-269, and eTRIM24. dTRIM24 suppresses growth to a greater extent than does IACS-9571, accompanied by apoptosis measured as enhanced PARP cleavage. In agreement with a sustained proliferative defect observed following dTRIM24 treatment, near-complete degradation of TRIM24 is observed in dTRIM24-treated cells throughout the duration of the experiment[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2170695-14-2
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Appearance Solid
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Molecular Weight 1113.30
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Formula C55H68N8O13S2
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Color White to off-white
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SMILES
CN(C1=C2C=C(C(NS(C3=CC(C(NCCOCCOCCOCC(N[C@@H](C(C)(C)C)C(N4[C@@H](C[C@H](C4)O)C(NCC5=CC=C(C=C5)C6=C(N=CS6)C)=O)=O)=O)=O)=CC=C3)(=O)=O)=C1)OC7=CC=CC(OCCC)=C7)C(N2C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (8)
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Journal Impact Factor
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Most Recent
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Mol Cell
TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains. [Abstract]2021 Apr 1;81(7):1411-1424.e7. PMID: 33567268 -
Proc Natl Acad Sci U S A
2025 Aug 19;122(33):e2507571122. PMID: 40815626 -
J Transl Med
Pharmacological targeting of Tripartite Motif Containing 24 for the treatment of glioblastoma. [Abstract]2021 Dec 9;19(1):505. PMID: 34886858 -
Cell Death Discov
Role of TRIM24 in the regulation of proteasome-autophagy crosstalk in bortezomib-resistant mantle cell lymphoma. [Abstract]2025 Mar 17;11(1):108. PMID: 40097385 -
Structure
PROTAC-mediated activation, rather than degradation, of a nuclear receptor reveals complex ligand-receptor interaction network. [Abstract]2024 Dec 5;32(12):2352-2363.e8. PMID: 39389062 -
Mol Carcinog
PRMT1 inhibition promotes ferroptosis sensitivity via ACSL1 upregulation in acute myeloid leukemia. [Abstract]2023 Aug;62(8):1119-1135. PMID: 37144835 -
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Solvent & Solubility
Methanol : 150 mg/mL (134.73 mM; Need ultrasonic)
DMSO : 100 mg/mL (89.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : < 1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
MOLM-13 cells are seeded at 30,000 cells/well. Growth over time of MOLM-13 cells treated with 5 μM of indicated compounds (e.g., dTRIM24) over 7 d. At endpoints, cells are suspended and mixed with Viacount reagent at 1:3. The mixture is incubated for 5 min, and viable cells are counted on the Guava easycyte flow cytometer. Means from three technical replicates of cell counts are calculated[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Methanol | 1 mM | 0.8982 mL | 4.4912 mL | 8.9823 mL | 22.4558 mL |
| 5 mM | 0.1796 mL | 0.8982 mL | 1.7965 mL | 4.4912 mL | |
| 10 mM | 0.0898 mL | 0.4491 mL | 0.8982 mL | 2.2456 mL | |
| 15 mM | 0.0599 mL | 0.2994 mL | 0.5988 mL | 1.4971 mL | |
| 20 mM | 0.0449 mL | 0.2246 mL | 0.4491 mL | 1.1228 mL | |
| 25 mM | 0.0359 mL | 0.1796 mL | 0.3593 mL | 0.8982 mL | |
| 30 mM | 0.0299 mL | 0.1497 mL | 0.2994 mL | 0.7485 mL | |
| 40 mM | 0.0225 mL | 0.1123 mL | 0.2246 mL | 0.5614 mL | |
| 50 mM | 0.0180 mL | 0.0898 mL | 0.1796 mL | 0.4491 mL | |
| 60 mM | 0.0150 mL | 0.0749 mL | 0.1497 mL | 0.3743 mL | |
| 80 mM | 0.0112 mL | 0.0561 mL | 0.1123 mL | 0.2807 mL | |
| Methanol | 100 mM | 0.0090 mL | 0.0449 mL | 0.0898 mL | 0.2246 mL |
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