Cat. No.: HY-138072

EMI1

Customer Review

Based on 1 Customer Validation

EMI1 is an EGFR ex19del/T790M/C797S and EGFR L858R/T790M/C797S inhibitor. EMI1 can be used for the research of mutant EGFR-associated, drug-resistant non-small-cell lung cancer (NSCLC).

For research use only. We do not sell to patients.

Purity: 99.45%
CAS No.: 35773-42-3
Formula: C₂₀H₁₈N₂O₃
Molecular Weight:334.37

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

EMI1 Chemical Structure

CAS No.: 35773-42-3

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Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

EMI1 is an EGFR ex19del/T790M/C797S and EGFR L858R/T790M/C797S inhibitor. EMI1 can be used for the research of mutant EGFR-associated, drug-resistant non-small-cell lung cancer (NSCLC)^[1].

IC₅₀ & Target^[1]

EGFR^{del19 T790M C797S}

EGFR^{L858R/T790M/C797S}

All EGFR Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
B16	IC₅₀	1.3 μM Compound: 9	Antiproliferative activity against mouse B16 cell line Antiproliferative activity against mouse B16 cell line	[PMID: 16793260]
DLD-1	IC₅₀	0.21 μM Compound: 9	Antiproliferative activity against human DLD1 cell line Antiproliferative activity against human DLD1 cell line	[PMID: 16793260]
HeLa	IC₅₀	1.2 μM Compound: 9	Antiproliferative activity against human HeLa cell line Antiproliferative activity against human HeLa cell line	[PMID: 16793260]
HUVEC	IC₅₀	0.12 μM Compound: 9	Antiproliferative activity against HUVEC cell line Antiproliferative activity against HUVEC cell line	[PMID: 16793260]
NIH3T3	IC₅₀	2.8 μM Compound: 9	Antiproliferative activity against mouse NIH3T3 cell line Antiproliferative activity against mouse NIH3T3 cell line	[PMID: 16793260]
SiHa	IC₅₀	2 μM Compound: 9	Antiproliferative activity against human SiHa cell line Antiproliferative activity against human SiHa cell line	[PMID: 16793260]
U-87MG ATCC	IC₅₀	0.21 μM Compound: 9	Antiproliferative activity against human U87 MG cell line Antiproliferative activity against human U87 MG cell line	[PMID: 16793260]

In Vitro

EMI1 inhibits PC9 EGFR ex19del/T790M/C797S organoid growth with the EC₅₀ of 131 nM^[1].
EMI1 (1 nM-10 μM) potently reduces the interaction of EGFR triple mutant with Shc1^[1].
EMI1 (1 nM-100μM) strongly inhibits the viability and increase the caspase 3/7 activity of PC9 EGFR ex19del/T790M/C797S triple-mutant cells than noncancerous human bronchial epithelial (HBE) cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

PC9 EGFR ex19del/T790M/C797S and HBE bronchial epithelial lung EGFR-WT control cells.
Concentration:

0.001, 0.01, 0.1, 1, 10, 100 μM
Incubation Time:

72 hours
Result:

Strongly inhibited the viability.

Chemical Information

CAS No. 35773-42-3
Appearance Solid
Molecular Weight 334.37
Formula C₂₀H₁₈N₂O₃
Color Light yellow to yellow
SMILES

O=C1C(C2=NC3=CC=CC=C3O2)=CC4=CC=C(N(CC)CC)C=C4O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (74.77 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9907 mL

14.9535 mL

29.9070 mL

5 mM

0.5981 mL

2.9907 mL

5.9814 mL

10 mM

0.2991 mL

1.4953 mL

2.9907 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Punit Saraon, et al. A drug discovery platform to identify compounds that inhibit EGFR triple mutants. Nat Chem Biol. 2020 May;16(5):577-586. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9907 mL	14.9535 mL	29.9070 mL	74.7675 mL
	5 mM	0.5981 mL	2.9907 mL	5.9814 mL	14.9535 mL
	10 mM	0.2991 mL	1.4953 mL	2.9907 mL	7.4767 mL
	15 mM	0.1994 mL	0.9969 mL	1.9938 mL	4.9845 mL
	20 mM	0.1495 mL	0.7477 mL	1.4953 mL	3.7384 mL
	25 mM	0.1196 mL	0.5981 mL	1.1963 mL	2.9907 mL
	30 mM	0.0997 mL	0.4984 mL	0.9969 mL	2.4922 mL
	40 mM	0.0748 mL	0.3738 mL	0.7477 mL	1.8692 mL
	50 mM	0.0598 mL	0.2991 mL	0.5981 mL	1.4953 mL
	60 mM	0.0498 mL	0.2492 mL	0.4984 mL	1.2461 mL

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EMI1

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Differentiation Inducing Compound Library

Anti-Hepatitis C Virus Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Cytokine Inhibitors Library

Anti-Drug-Resistant Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

All EGFR Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

EMI1

Immunology/Inflammation Compound Library

JAK/STAT Compound Library

Kinase Inhibitor Library

Protein Tyrosine Kinase Compound Library

Anti-Cancer Compound Library

Differentiation Inducing Compound Library

Anti-Hepatitis C Virus Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Highly Selective Inhibitors Library

Cytokine Inhibitors Library

Anti-Drug-Resistant Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Brain Cancer Compound Library

Protein Kinase Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

All EGFR Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

EMI1 Related Classifications

Cat. No.: HY-138072