Formononetin
Based on 24 publication(s) in Google Scholar
Formononetin is a potent FGFR2 inhibitor with an IC50 of ~4.31 μM. Formononetin potently inhibits angiogenesis and tumor growth.
For research use only. We do not sell to patients.
- Purity: 99.21%
- CAS No.: 485-72-3
- Formula: C16H12O4
- Molecular Weight:268.26
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
Publications Citing Use of MedChemExpress (MCE) Formononetin
More- Acta Pharm Sin B. 2021 Jan;11(1):143-155. [Abstract]
- Food Chem. 2025 Dec 30:497:146992. [Abstract]
- Food Chem. 2025 May 31:489:144992. [Abstract]
- Phytomedicine. 2025 Oct 14:148:157408. [Abstract]
- Phytomedicine. 2025 Aug 15:147:157152. [Abstract]
- Phytomedicine. 2025 Jun 9:145:156960. [Abstract]
- Mol Med. 2019 Dec;20(6):4984-4992. [Abstract]
- Phytother Res. 2023 Aug;37(8):3363-3379. [Abstract]
- Ind Crops Prod. 2025 Dec 11;239:122458.
- Chin Med. 2026 Jan 12;21(1):29. [Abstract]
- Am J Chin Med. 2024;52(5):1487-1505. [Abstract]
- Int J Mol Sci. 2024 Aug 2;25(15):8471. [Abstract]
- Biosci Rep. 2020 Oct 30;40(10):BSR20201349. [Abstract]
- Ann Med. 2024 Dec;56(1):2404550. [Abstract]
- Fish Shellfish Immunol. 2025 Aug 6:166:110633. [Abstract]
- Mol Med Rep. 2020 Mar;21(3):1192-1200. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2024 Sep;397(9):6837-6850. [Abstract]
- Mol Immunol. 2026 Feb:190:1-10. [Abstract]
- Genomics. 2021 Jul;113(4):2702-2716. [Abstract]
- Genes (Basel). 2024 Sep 8;15(9):1180. [Abstract]
- Tissue Cell. 2025 Dec 9:99:103271. [Abstract]
- Biochem Biophys Res Commun. 2020 May 7;525(3):759-766. [Abstract]
- Biomed Chromatogr. 2019 Sep;33(9):e4563. [Abstract]
- Phytomed Plus. 2023 Sep 1, 100483.
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Histological Imaging/Staining
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Cell Imaging/Staining
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IF
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Histological Imaging/Staining
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WB
Biological Activity
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FGFR2 4.31 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | ED50 |
2.87 μg/mL
Compound: 11
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Cytotoxicity against human A549 cells after 7 days
Cytotoxicity against human A549 cells after 7 days
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[PMID: 8201311] |
| A549 | IC50 |
>100 μM
Compound: 13
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Cytotoxicity against human A549 cells after 72 hrs by MTT assay
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
|
[PMID: 18440233] |
| B16-BL6 | IC50 |
>100 μM
Compound: 13
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Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
|
[PMID: 18440233] |
| BV-2 | IC50 |
>100 μM
Compound: 19
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Anti-neuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
Anti-neuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
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[PMID: 25466192] |
| BV-2 | IC50 |
>50 μM
Compound: 9
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Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production
Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production
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[PMID: 29482940] |
| BV-2 | IC50 |
13.19 μM
Compound: 2
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Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
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[PMID: 28911817] |
| DU-145 | IC50 |
>100 μM
Compound: Formononetin
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Cytotoxicity against human DU145 cells after 72 hrs by MTT assay
Cytotoxicity against human DU145 cells after 72 hrs by MTT assay
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[PMID: 22633834] |
| ECa-109 cell line | IC50 |
>100 μM
Compound: 1
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Cytotoxicity against human EC109 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human EC109 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
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[PMID: 28038329] |
| HCT-116 | GI50 |
64 μM
Compound: 12e
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Antiproliferative activity against human HCT116 cell line
Antiproliferative activity against human HCT116 cell line
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[PMID: 16789754] |
| HCT-116 | IC50 |
60.97 μM
Compound: Formononetin
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Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay
Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay
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[PMID: 22633834] |
| HeLa | IC50 |
>100 μM
Compound: 13
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Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
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[PMID: 18440233] |
| HeLa | IC50 |
8.51 μM
Compound: Formononetin
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Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
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[PMID: 22633834] |
| HepG2 | IC50 |
60.5 μM
Compound: 15
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Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
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[PMID: 24974349] |
| HT-1080 | IC50 |
>100 μM
Compound: 13
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Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
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[PMID: 18440233] |
| HT-29 | ED50 |
6.43 μg/mL
Compound: 11
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Cytotoxicity against human HT-29 cells after 7 days
Cytotoxicity against human HT-29 cells after 7 days
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[PMID: 8201311] |
| HT-29 | GI50 |
74 μM
Compound: 12e
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Antiproliferative activity against human HT29 cell line
Antiproliferative activity against human HT29 cell line
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[PMID: 16789754] |
| Lewis lung carcinoma cell line | IC50 |
>100 μM
Compound: 13
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Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
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[PMID: 18440233] |
| MCF7 | ED50 |
1.96 μg/mL
Compound: 11
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Cytotoxicity against human MCF7 cells after 7 days
Cytotoxicity against human MCF7 cells after 7 days
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[PMID: 8201311] |
| MCF7 | GI50 |
81.4 μM
Compound: 12e
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Antiproliferative activity against human MCF7 cell line
Antiproliferative activity against human MCF7 cell line
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[PMID: 16789754] |
| MDA-MB-468 | GI50 |
27.2 μM
Compound: 12e
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Antiproliferative activity against human MDA-MB-468 cell line
Antiproliferative activity against human MDA-MB-468 cell line
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[PMID: 16789754] |
| MDCK | CC50 |
123 μM
Compound: 3
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Cytotoxicity against MDCK cells after 72 hrs
Cytotoxicity against MDCK cells after 72 hrs
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[PMID: 24313801] |
| MDCK | IC50 |
38.2 μM
Compound: 3
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Antiviral activity against Influenza A virus J/8178/09 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs
Antiviral activity against Influenza A virus J/8178/09 infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs
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[PMID: 24313801] |
| MDCK | IC50 |
42.6 μM
Compound: 3
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Antiviral activity against Influenza A virus (A/Hong Kong/1/1968(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs
Antiviral activity against Influenza A virus (A/Hong Kong/1/1968(H3N2)) infected in MDCK cells assessed as inhibition of virus-induced cytopathic effect after 48 hrs
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[PMID: 24313801] |
| MGC-803 | IC50 |
>100 μM
Compound: 1
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Cytotoxicity against human MGC803 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human MGC803 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
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[PMID: 28038329] |
| N9 | IC50 |
>100 μM
Compound: 4
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Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
Antineuroinflammatory activity in mouse N9 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
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[PMID: 28073678] |
| Panel NCI-60 (60 carcinoma cell lines) | GI50 |
50 μM
Compound: 12e
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Antiproliferative activity against human NCI60 cell line
Antiproliferative activity against human NCI60 cell line
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[PMID: 16789754] |
| PC-3 | IC50 |
57.01 μM
Compound: 1
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Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
Cytotoxicity against human PC3 cells assessed as inhibition of cell viability after 48 hrs by MTT assay
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[PMID: 28038329] |
| RAW264.7 | IC50 |
56.1 μM
Compound: 6
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
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[PMID: 23743282] |
| SGC-7901 | IC50 |
2.17 μM
Compound: Formononetin
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Cytotoxicity against human SGC7901 cells after 72 hrs by MTT assay
Cytotoxicity against human SGC7901 cells after 72 hrs by MTT assay
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[PMID: 22633834] |
| SH-SY5Y | IC50 |
>100 μM
Compound: Formononetin
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Cytotoxicity against human SH-SY5Y cells after 72 hrs by MTT assay
Cytotoxicity against human SH-SY5Y cells after 72 hrs by MTT assay
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[PMID: 22633834] |
| U-937 | IC50 |
44 μM
Compound: 6, formononetin
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Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
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[PMID: 17158054] |
| U-937 | IC50 |
45 μM
Compound: 6, formononetin
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Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
|
[PMID: 17158054] |
| U-937 | IC50 |
50 μM
Compound: 6, formononetin
|
Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
|
[PMID: 17158054] |
Formononetin is one of the major isoflavonoid constituents isolated from Astragalus membranaceus and has been demonstrated diverse pharmacological benefits. Formononetin possesses anti-angiogenic activity in human colon cancer cells. Formononetin also promotes cell cycle arrest via downregulation of Akt/Cyclin D1/CDK4 in human prostate cancer cells[1].
Formononetin (25 to 150 μM) markedly decreases the proliferation of endothelial cells stimulated by FGF2[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HUVECs
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Concentration:0, 10, 25, 50, 75, 100, and 150 μM
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Incubation Time:
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Result:Significantly decreased the proliferation of HUVECs stimulated by FGF2 in a dose-dependent manner, while had little inhibitory effects on HUVECs that were not stimulated by FGF2.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude mice bearing MDA-MB-231 xenografts[1]
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Dosage:100 mg/kg
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Administration:Treated daily by intragastric administration for 25 days
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Result:Inhibited breast cancer growth and angiogenesis in vivo.
Chemical Information
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CAS No. 485-72-3
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Appearance Solid
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Molecular Weight 268.26
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Formula C16H12O4
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Color White to off-white
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SMILES
O=C1C(C2=CC=C(OC)C=C2)=COC3=CC(O)=CC=C13
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Synonyms
Biochanin B; Flavosil; Formononetol
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (24)
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Journal Impact Factor
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Most Recent
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Acta Pharm Sin B
Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]2021 Jan;11(1):143-155. PMID: 33532186 -
Food Chem
Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]2025 Dec 30:497:146992. PMID: 41285060 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 May 31:489:144992. PMID: 40466530 -
Phytomedicine
Formononetin mitigates alcoholic liver disease by restoring ALDH2 function and inducing ERK1/2-Nrf2 antioxidant signaling. [Abstract]2025 Oct 14:148:157408. PMID: 41110354 -
Phytomedicine
Formononetin, a bioactive isoflavone compound in modified nuanxinkang, alleviates cardiorenal syndrome type 4-induced mitochondrial dysfunction by targeting Hippo-YAP signaling. [Abstract]2025 Aug 15:147:157152. PMID: 40850069 -
Phytomedicine
Formononetin enhances cisplatin chemotherapy sensitivity in osteosarcoma by inducing ferroptosis and reconstructing the immune microenvironment. [Abstract]2025 Jun 9:145:156960. PMID: 40543234
Formononetin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun 9:145:156960. [Abstract]
Formononetin (25-100 mg/kg, i.g.) administration ameliorated Ethanol-Induced liver injury in 12 weeks aged mice. Representative images of H&E staining.
Formononetin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun 9:145:156960. [Abstract]
Formononetin (25-100 mg/kg, i.g.) administration ameliorated Ethanol-Induced liver injury in 12 weeks aged mice. Representative images of TUNEL staining.
Formononetin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Jun 9:145:156960. [Abstract]
Formononetin (25-100 mg/kg, i.g.) protected against Ethanol induced aldehyde toxicity in 12 weeks aged mice. Representative images of Dihydroethidium (DHE) staining and 4-Hydroxynonenal (4-HNE) staining.
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Mol Med
Formononetin attenuates monocrotaline‑induced pulmonary arterial hypertension via inhibiting pulmonary vascular remodeling in rats. [Abstract]2019 Dec;20(6):4984-4992. PMID: 31702810
Formononetin purchased from MedChemExpress. Usage Cited in: Mol Med. 2019 Dec;20(6):4984-4992. [Abstract]
Formononetin (10-60 mg/kg, i.p., daily for 2 weeks) suppressed Monocrotaline (MCT)-induced pulmonary vascular remodeling in male Sprague-Dawley (7-weeks-old) rats.
Formononetin purchased from MedChemExpress. Usage Cited in: Mol Med. 2019 Dec;20(6):4984-4992. [Abstract]
Formononetin (10-60 mg/kg, i.p., daily for 2 weeks) attenuated decreases in Bax and cleaved caspase-3 expression induced by Monocrotaline (MCT).
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Phytother Res
Formononetin improves the inflammatory response and bone destruction in knee joint lesions by regulating the NF-kB and MAPK signaling pathways. [Abstract]2023 Aug;37(8):3363-3379. PMID: 37002905 -
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Chin Med
Therapeutic potential of Sheng-Xian-Tang in doxorubicin-induced chronic heart failure by regulation of phenylalanine metabolism disruption. [Abstract]2026 Jan 12;21(1):29. PMID: 41527105 -
Am J Chin Med
Formononetin Alleviates Ischemic Acute Kidney Injury by Regulating Macrophage Polarization through KLF6/STAT3 Pathway. [Abstract]2024;52(5):1487-1505. PMID: 39169449 -
Int J Mol Sci
Formononetin Defeats Multidrug-Resistant Cancers by Induction of Oxidative Stress and Suppression of P-Glycoprotein. [Abstract]2024 Aug 2;25(15):8471. PMID: 39126039 -
Biosci Rep
Experimental evidence and network pharmacology-based analysis reveal the molecular mechanism of Tongxinluo capsule administered in coronary heart diseases. [Abstract]2020 Oct 30;40(10):BSR20201349. PMID: 32990315 -
Ann Med
In silico and in vivo verification of the mechanism of formononetin in treating hepatocellular carcinoma. [Abstract]2024 Dec;56(1):2404550. PMID: 39301883 -
Fish Shellfish Immunol
Emodin enhances host antiviral immunity against Micropterus salmoides rhabdovirus by activating RLR signaling in largemouth bass. [Abstract]2025 Aug 6:166:110633. PMID: 40769268 -
Mol Med Rep
Inhibitory effects of formononetin on the monocrotaline‑induced pulmonary arterial hypertension in rats. [Abstract]2020 Mar;21(3):1192-1200. PMID: 31922224 -
Naunyn Schmiedebergs Arch Pharmacol
Mechanism of Sophorae Flavescentis Radix against ovarian cancer via new pharmacology, molecular docking, and experimental verification. [Abstract]2024 Sep;397(9):6837-6850. PMID: 38561549 -
Mol Immunol
Formononetin in Jiawei Qihuangyin inhibits podocyte epithelial-mesenchymal transition and ameliorates diabetic nephropathy via SIRT1/NF-κB axis. [Abstract]2026 Feb:190:1-10. PMID: 41483656 -
Genomics
Transcriptomics and metabolomics reveal the induction of flavonoid biosynthesis pathway in the interaction of Stylosanthes-Colletotrichum gloeosporioides. [Abstract]2021 Jul;113(4):2702-2716. PMID: 34111523 -
Genes (Basel)
Integration of Transcriptomics and WGCNA to Characterize Trichoderma harzianum-Induced Systemic Resistance in Astragalus mongholicus for Defense against Fusarium solani. [Abstract]2024 Sep 8;15(9):1180. PMID: 39336771 -
Tissue Cell
Niaodukang for the treatment of chronic kidney disease: Regulation of the TRAF3/NF-κB2 signaling pathway and its improvement of the intestinal barrier. [Abstract]2025 Dec 9:99:103271. PMID: 41401720 -
Biochem Biophys Res Commun
Formononetin ameliorates myocardial ischemia/reperfusion injury in rats by suppressing the ROS-TXNIP-NLRP3 pathway. [Abstract]2020 May 7;525(3):759-766. PMID: 32145915 -
Biomed Chromatogr
Comparative pharmacokinetic study on three formulations of Astragali Radix by an LC-MS/MS method for determination of formononetin in human plasma. [Abstract]2019 Sep;33(9):e4563. PMID: 31025385 -
Solvent & Solubility
DMSO : ≥ 35 mg/mL (130.47 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (9.32 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (9.32 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7277 mL | 18.6386 mL | 37.2773 mL | 93.1932 mL |
| 5 mM | 0.7455 mL | 3.7277 mL | 7.4555 mL | 18.6386 mL | |
| 10 mM | 0.3728 mL | 1.8639 mL | 3.7277 mL | 9.3193 mL | |
| 15 mM | 0.2485 mL | 1.2426 mL | 2.4852 mL | 6.2129 mL | |
| 20 mM | 0.1864 mL | 0.9319 mL | 1.8639 mL | 4.6597 mL | |
| 25 mM | 0.1491 mL | 0.7455 mL | 1.4911 mL | 3.7277 mL | |
| 30 mM | 0.1243 mL | 0.6213 mL | 1.2426 mL | 3.1064 mL | |
| 40 mM | 0.0932 mL | 0.4660 mL | 0.9319 mL | 2.3298 mL | |
| 50 mM | 0.0746 mL | 0.3728 mL | 0.7455 mL | 1.8639 mL | |
| 60 mM | 0.0621 mL | 0.3106 mL | 0.6213 mL | 1.5532 mL | |
| 80 mM | 0.0466 mL | 0.2330 mL | 0.4660 mL | 1.1649 mL | |
| 100 mM | 0.0373 mL | 0.1864 mL | 0.3728 mL | 0.9319 mL |