Fanotaprim (Synonyms: VYR-006)

Name: Fanotaprim
Brand: MedChemExpress
SKU: HY-137439
Rating: 4.5 (1 reviews)

Cat. No.: HY-137439 Purity: 98.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Fanotaprim is a dihydrofolate reductase (DHFR) inhibitor with IC₅₀s of 1.57 and 308 nM for tgDHFR (Toxoplasma gondii DHFR) and hDHFR (human DHFR), respectively. Fanotaprim has the potential for the research of toxoplasmosis.

For research use only. We do not sell to patients.

Fanotaprim Chemical Structure

CAS No. : 2120282-75-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 264	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 264	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1160	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1850	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

In Vitro

Fanotaprim shows parasiticidal and antiproliferative effects with EC₅₀s of 13 and 7300 nM against the type I RH strain of T. gondii and MCF-7 cells, respectively^[1].
Fanotaprim shows ability to inhibit the growth of T. gondii strains in vitro with EC₅₀s ranging 7.6~ 29.8 nM (GT1, ME49, CTG, RUB and VAND)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Fanotaprim (1-10 mg/kg; p.o.; daily; beginning on day 1 through day 7) shows highly effective in control of acute infection by highly virulent strains of T. gondii in the murine model^[1].
Fanotaprim (1mg/kg; i.v; mouse) shows C_L, V_d, and t_1/2 values of 10.6 mL/min/kg, 1.14 L/kg, and 3.9 hours, respectively^[1].
Fanotaprim (0.83 mg/kg; p.o; mouse) shows F, C_max, T_max, and AUC_0-last of 47.3%, 178 ng/mL, 0.05 hours and 750 ng h/mL, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	CD-1female mice (murine model of acute toxoplasmosis)^[1]
Dosage:	1-10 mg/kg
Administration:	p.o.; daily; beginning on day 1 through day 7
Result:	Mice were monitored for survival for 30 days within termittent IVIS monitoring. At doses of 10 mg/kg Fanotaprim, q.d. or b.i.d. for 7 days, yielded 100% survival for 30 days.

Molecular Weight

378.43

Formula

C₁₉H₂₂N₈O

CAS No.

2120282-75-7

Appearance

Solid

Color

Off-white to light yellow

SMILES

NC1=NC=C(N2CCN(C3=CC=CC(C4=CN=C(OC)N=C4)=C3)CC2)C(N)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (88.07 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6425 mL	13.2125 mL	26.4250 mL
5 mM	0.5285 mL	2.6425 mL	5.2850 mL
10 mM	0.2642 mL	1.3212 mL	2.6425 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.89%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (245 KB) HNMR (276 KB) LCMS (270 KB)

Handling Instructions (2659 KB)

References

[1]. Hopper AT, et al. Discovery of Selective Toxoplasma gondii Dihydrofolate Reductase Inhibitors for the Treatment of Toxoplasmosis. J Med Chem. 2019;62(3):1562-1576. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6425 mL	13.2125 mL	26.4250 mL	66.0624 mL
	5 mM	0.5285 mL	2.6425 mL	5.2850 mL	13.2125 mL
	10 mM	0.2642 mL	1.3212 mL	2.6425 mL	6.6062 mL
	15 mM	0.1762 mL	0.8808 mL	1.7617 mL	4.4042 mL
	20 mM	0.1321 mL	0.6606 mL	1.3212 mL	3.3031 mL
	25 mM	0.1057 mL	0.5285 mL	1.0570 mL	2.6425 mL
	30 mM	0.0881 mL	0.4404 mL	0.8808 mL	2.2021 mL
	40 mM	0.0661 mL	0.3303 mL	0.6606 mL	1.6516 mL
	50 mM	0.0528 mL	0.2642 mL	0.5285 mL	1.3212 mL
	60 mM	0.0440 mL	0.2202 mL	0.4404 mL	1.1010 mL
	80 mM	0.0330 mL	0.1652 mL	0.3303 mL	0.8258 mL

Fanotaprim Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fanotaprim2120282-75-7VYR-006VYR006VYR 006AntifolatedhydrofolatereductaseDHFRtoxoplasmosissurvivalCD-1miceInhibitorinhibitorinhibit

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Fanotaprim (Synonyms: VYR-006)

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Fanotaprim Related Antibodies

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Fanotaprim Related Antibodies

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Complete Stock Solution Preparation Table

Fanotaprim Related Classifications

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