1. Apoptosis
  2. Apoptosis
  3. Fluorizoline

Fluorizoline 

Cat. No.: HY-114989 Purity: 99.77%
Handling Instructions

Fluorizoline selectively and directly binds to prohibitin 1 (PHB1) and 2 (PHB2), and induces apoptosis. Fluorizoline reduces chronic lymphocytic leukemia (CLL) cell viability through the upregulation of NOXA and BIM. fluorizoline exerts antitumor action in a p53-independent manner.

For research use only. We do not sell to patients.

Fluorizoline Chemical Structure

Fluorizoline Chemical Structure

CAS No. : 1362243-70-6

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10 mM * 1 mL in DMSO Check price and availability
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Description

Fluorizoline selectively and directly binds to prohibitin 1 (PHB1) and 2 (PHB2), and induces apoptosis. Fluorizoline reduces chronic lymphocytic leukemia (CLL) cell viability through the upregulation of NOXA and BIM. fluorizoline exerts antitumor action in a p53-independent manner[1].

In Vitro

Fluorizoline (1.25-20 μM; 24 hours) induces apoptosis in primary CLL cells ex vivo[1].
Fluorizoline (1.25-20 μM; 24 hours) causes an increase of NOXA protein levels. NOXA levels are not modified after fluorizoline treatment, neither at the initial stages nor at 24 hours of incubation of CLL cells[1].
Fluorizoline reduces the percentage of viable normal B and T cells (48.6% and 82.8% of viable cells at 24 hours of treatment with 10 μM Fluorizoline in normal CD19+ and CD3+ populations, respectively) with a mean EC50 value of 10.9 μM and 19.1 μM at 24 hours for normal B and T cells, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[1]

Cell Line: Primary CLL cells
Concentration: 1.25 to 20 μM
Incubation Time: 24 hours
Result: Strongly reduced cell viability and induced apoptosis in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: Primary CLL cells
Concentration: 10 μM
Incubation Time: 2, 4, 8, 24 hours
Result: Caused an increase of NOXA protein levels.
In Vivo

Fluorizoline (15 mg/kg; ip; three times a week for five weeks) becomes very rapidly (3 weeks) leukemic, as reflected by the increase in the percentage and the number of CD5+CD19+ CLL cells in the blood in 6-week-old recipient C57BL/6 Eμ-TCL1 mouse model of CLL. Fluorizoline does not induce apoptosis in vivo. Fluorizoline does not control disease development in the spleen as indicated by enlarged spleens[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

362.20

Formula

C₁₅H₈Cl₂F₃NS

CAS No.

1362243-70-6

SMILES

FC1(C(F)(N=C(S1)C2=CC=C(C=C2)Cl)F)C3=CC=C(C=C3)Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (138.05 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7609 mL 13.8045 mL 27.6091 mL
5 mM 0.5522 mL 2.7609 mL 5.5218 mL
10 mM 0.2761 mL 1.3805 mL 2.7609 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (6.90 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (6.90 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

FluorizolineApoptosisprohibitinPHB1PHB2ChroniclymphocyticleukemiaCLLNOXABIMp53Inhibitorinhibitorinhibit

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Fluorizoline
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