2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. FRC-303

FRC-303 is a CHD1 inhibitor with a Kd of 0.14 μM and an IC50 of 0.18 μM. FRC-303 binds to the H3K4me3 binding site of CHD1 tandem chromodomain, forms aromatic cage interactions and extended ligand contacts, acts as a methyl-lysine mimic, and occupies natural peptide ligand-binding regions. FRC-303 can be used for the research of prostate cancer.

For research use only. We do not sell to patients.

FRC-303

FRC-303 Chemical Structure

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

FRC-303 Related Antibodies

Top Publications Citing Use of Products

View All DNA/RNA Synthesis Isoform Specific Products:

View All Isoforms
RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

FRC-303 is a CHD1 inhibitor with a Kd of 0.14 μM and an IC50 of 0.18 μM. FRC-303 binds to the H3K4me3 binding site of CHD1 tandem chromodomain, forms aromatic cage interactions and extended ligand contacts, acts as a methyl-lysine mimic, and occupies natural peptide ligand-binding regions. FRC-303 can be used for the research of prostate cancer[1].

In Vitro

FRC-303 (Compound 2s) binds to purified recombinant CHD1 tandem chromodomain with a Kd of 0.14 μM[1].
FRC-303 inhibits the interaction between purified recombinant CHD1 tandem chromodomain and H3K4me3 peptide in a TR-FRET assay with an IC50 of 0.18 μM[1].
FRC-303 engages exogenous CHD1 tandem chromodomain in transfected HEK293T cells with an EC50 of 15 μM in a NanoBRET assay[1].
FRC-303 (25-100 μM) dose-dependently inhibits binding of endogenous CHD1 to LSD1-K114me3 peptide in HEK293T cell lysates[1].
FRC-303 (10 μM) does not inhibit the catalytic activity of purified LSD1, KMT9, METTL21A, EHMT2 (G9a), SET8, or NSD2 enzymes[1].
FRC-303 (16 μM) does not inhibit H3K4me3 peptide binding to purified TAF3, SPIN1, or PHF8 reader domains[1].
FRC-303 (0.8-6 μM; 48 h) impairs viability of PC-3M-Luc prostate cancer cells, with viability reduced to ~5% at 6 μM[1].
FRC-303 (0.8-6 μM; 48 h) potently impairs viability of 22Rv1 prostate cancer cells, with viability reduced to ~5% or lower at concentrations ≥2.7 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FRC-303 Related Antibodies

Cell Viability Assay[1]

Cell Line: PC-3M-Luc prostate cancer cells
Concentration: 0.8 μM; 1.2 μM; 1.8 μM; 2.7 μM; 4 μM; 6 μM
Incubation Time: 48 h
Result: Maintained viability ≥100% at 0.8 μM and 1.2 μM.
Decreased viability to ~115% at 1.8 μM, ~40% at 2.7 μM, ~10% at 4 μM, and ~5% at 6 μM relative to DMSO control.

Cell Viability Assay[1]

Cell Line: 22Rv1 prostate cancer cells
Concentration: 0.8 μM; 1.2 μM; 1.8 μM; 2.7 μM; 4 μM; 6 μM
Incubation Time: 48 h
Result: Maintained viability ~100% at 0.8 μM and ~90% at 1.2 μM.
Decreased viability to ~10% at 1.8 μM, and remained at ~5% or lower at 2.7 μM, 4 μM, and 6 μM relative to DMSO control.
Molecular Weight

704.90

Formula

C41H52N8O3
SMILES

COCCN1N=NC(COC2=CC=C(C=C2)CNC3CCN(C4=NC5=CC(OCC)=CC=C5C(NC6CCN(CC7=CC=CC=C7)CC6)=C4)CC3)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

FRC-303 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FRC-303FRC303FRC 303DNA/RNA Synthesisprostate cancerHEK293T cellsEHMT2LSD1PC-3M-Luc prostate cancer cellsH3K4me3KMT9METTL21A22Rv1 prostate cancer cellsCHD1 tandem chromodomainInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
FRC-303
Cat. No.:
HY-183602
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.