ODN 1668
Based on 1 publication(s) in Google Scholar
ODN 1668, a class B CpG ODN (oligodeoxynucleotide), is a TLR-9 agonist. ODN 1668 has strong immune regulatory properties, can enhance the level of antibody IgG2 subtype, promote the immune response of T cells and B cells, and can be used in the study of vaccine adjuvants. In addition, CpG ODN 1668 induces an antimicrobial immune response via a CaTLR9 dependent pathway in groupers. Sequence: 5'-tccatgacgttcctgatgct-3’.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.03%
- No. CAS: 1186063-66-0
- Peso molecular:6364.10
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Almacenamiento:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) ODN 1668
More
Actividad biológica
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TLR9 |
ODN 1668 (6-60 µg/mL; 12-72 h) increases the synthesis of S100A8/A9, IL-6 and β-galactosidase in mouse bone marrow derived macrophages[1].
ODN 1668 (24 h) promotes the activation and proliferation of B cells and increases the expression of CD86 and CD25[2].
ODN 1668 (0.3-3.0 μM; 4 days) promotes cell growth in anti-CD3-treated CD8+ T cells in a dose-dependent manner[2].
ODN 1668 (0.25-32 μg/mL; 12 h) promotes the proliferation of head kidney lymphocytes and enhances the expression of immune genes[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Head kidney lymphocytes
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Concentration:2 μg/mL
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Incubation Time:12 h
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Result:Increased the mRNA levels of TLR9, IL-1β, IL-6, IL-8, TNF-α, IFN-γ, MHC-Iα and MHC-IIα.
ODN 1668 (15 μg; intramuscular injection; single dose) improves the antibacterial immunity of Epinephelus laminatus infection, and has the most significant antibacterial activity in liver, spleen and kidney[3].
ODN 1668 (1-5 mg/kg; intraperitoneal/subcutaneous injection; single dose) can cause fever, anorexia and brain inflammation in rats[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar rats (175-200 g)[4]
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Dosage:1 and 5 mg/kg
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Administration:Intraperitoneal and subcutaneous injection, single dose
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Result:Caused moderate fever and anorexia.
Induced a significant increase of IL-6.
Increased expression of inflammatory genes in the hypothalamus (SOCS3, PGE2, COX-2 and mPGES).
Activated inflammatory transcription factors in the rat brain (STAT3 and NF-IL6).
Chemical Information
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No. CAS 1186063-66-0
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Appearance Solid
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Peso molecular 6364.10
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Color White to off-white
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SMILES
[DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-T-G-C-T)]
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Sequence
DNA, d(P-thio)(T-C-C-A-T-G-A-C-G-T-T-C-C-T-G-A-T-G-C-T)
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Methods
Genetically encoded biosensor for monitoring spatiotemporal dynamics of CCR2 ligands in culture and in vivo. [Abstract]2025 Aug;22(8):1731-1741. PMID: 40665002
Solvente y solubilidad
H2O : ≥ 20 mg/mL (3.14 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2242 KB)
Referencias
[1]. Rattanaprukskul K, et al. TLR9 Mediates Periodontal Aging by Fostering Senescence and Inflammaging. J Dent Res. 2022 Dec;101(13):1628-1636. [Content Brief]
[2]. Lipford GB, et al. CpG-containing synthetic oligonucleotides promote B and cytotoxic T cell responses to protein antigen: a new class of vaccine adjuvants. Eur J Immunol. 1997 Sep;27(9):2340-4. [Content Brief]
[3]. Chen X, et al. CpG ODN 1668 as TLR9 agonist mediates humpback grouper (Cromileptes altivelis) antibacterial immune responses. Fish Shellfish Immunol. 2023 Jul;138:108839. [Content Brief]
[4]. Damm J, et al. Intraperitoneal and subcutaneous injections of the TLR9 agonist ODN 1668 in rats: brain inflammatory responses are related to peripheral IL-6 rather than interferons. J Neuroimmunol. 2014 Dec 15;277(1-2):105-17. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.1571 mL | 0.7857 mL | 1.5713 mL | 3.9283 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
ODN 1668 Related Classifications
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