RPA1 - replication protein A1 Gene

Also Known as HSSB; RF-A; RP-A; REPA1; RPA70; MST075; PFBMFT6

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 6117

About RPA1

Cytogenetic location: 17p13.3 Genomic coordinates (GRCh38): 17:1,830,005-1,900,082 (from NCBI)

This gene has 7 transcripts (splice variants), 268 orthologues and is associated with 1 phenotype. Ubiquitous expression in lymph node (RPKM 21.6), ovary (RPKM 19.7) and 25 other tissues.

Summary

This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. [provided by RefSeq, Sep 2017]

RPA1 Products (3)

mRNA	Protein	Name
NM_001355120.2	NP_001342049.1	replication protein A 70 kDa DNA-binding subunit isoform 2
NM_001355121.2	NP_001342050.1	replication protein A 70 kDa DNA-binding subunit isoform 3
NM_002945.5	NP_002936.1	replication protein A 70 kDa DNA-binding subunit isoform 1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables G-rich strand telomeric DNA binding	IDA IDA: Inferred from direct assay	16973897	GOA
enables G-rich strand telomeric DNA binding	IMP IMP: Inferred from mutant phenotype	26041456	GOA
enables chromatin-protein adaptor activity	IDA IDA: Inferred from direct assay	35597237	GOA
enables damaged DNA binding	IDA IDA: Inferred from direct assay	7700386	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	1311258	GOA
enables single-stranded DNA binding	IDA IDA: Inferred from direct assay	8990123	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in DNA damage response	IMP IMP: Inferred from mutant phenotype	27248496	GOA
involved in DNA repair	IDA IDA: Inferred from direct assay	20545304	GOA
involved in DNA repair	IMP IMP: Inferred from mutant phenotype	18469000	GOA
involved in DNA replication	IMP IMP: Inferred from mutant phenotype	9430682	GOA
involved in base-excision repair	IDA IDA: Inferred from direct assay	9765279	GOA
involved in double-strand break repair via homologous recombination	IMP IMP: Inferred from mutant phenotype	17765923	GOA
involved in mismatch repair	IMP IMP: Inferred from mutant phenotype	9430682	GOA
involved in nucleotide-excision repair	IMP IMP: Inferred from mutant phenotype	9430682	GOA
involved in protein localization to chromosome	IDA IDA: Inferred from direct assay	27723717	GOA
involved in protein localization to site of double-strand break	IDA IDA: Inferred from direct assay	35597237	GOA
involved in telomere maintenance	IMP IMP: Inferred from mutant phenotype	17959650	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of DNA replication factor A complex	IDA IDA: Inferred from direct assay	11927569	GOA
part of DNA replication factor A complex	IPI IPI: Inferred from physical interaction	7760808	GOA
colocalizes with PML body	IDA IDA: Inferred from direct assay	17959650	GOA
located in PML body	IDA IDA: Inferred from direct assay	16135809	GOA
located in nucleus	IDA IDA: Inferred from direct assay	17959650	GOA
located in site of DNA damage	IMP IMP: Inferred from mutant phenotype	27248496	GOA
is active in site of double-strand break	IDA IDA: Inferred from direct assay	35597237	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RPA1 Protein Structure

Rep-A_N

tRNA_anti-codon

Rep_fac-A_C

0
100
200
300
400
500
616 a.a.

Protein Preferred Names	Protein Names
replication protein A 70 kDa DNA-binding subunit	MSTP075

RPA1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	RPA1	P27694	PRIMPOL	Homo sapiens	Q96LW4	Anti Tag CoIP	24126761
Intra	RPA1	P27694	PRIMPOL	Homo sapiens	Q96LW4	TAP	24126761
Intra	RPA1	P27694	PRIMPOL	Homo sapiens	Q96LW4	Pull Down	24126761
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59-3	Y2H Prey Pooling	32296183
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59-3	Validated Y2H	32296183
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59-3	Y2H Array	32296183
Intra	RPA1	P27694	RPA4	Homo sapiens	Q13156	Anti Bait CoIP	7760808
Intra	RPA1	P27694	RPA4	Homo sapiens	Q13156	Y2H	7760808
Intra	RPA1	P27694	HSPA6	Homo sapiens	P17066	Y2H Prey Pooling	32296183
Intra	RPA1	P27694	HSPA6	Homo sapiens	P17066	Y2H Array	32296183
Intra	RPA1	P27694	WRN	Homo sapiens	Q14191	Pull Down	15965237
Intra	RPA1	P27694	WRN	Homo sapiens	Q14191	TAP	24126761
Intra	RPA1	P27694	WRN	Homo sapiens	Q14191	ELISA	15965237
Intra	RPA1	P27694	WRN	Homo sapiens	Q14191	ELISA	15735006
Cross	RPA1	P27694	lt_sv40	Simian virus 40	P03070	CoIP	17666013
Cross	RPA1	P27694	lt_sv40	Simian virus 40	P03070	Experimental Interac	8381428
Intra	RPA1	P27694	BLM	Homo sapiens	P54132	TAP	24126761
Intra	RPA1	P27694	BLM	Homo sapiens	P54132	ELISA	15965237
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	TAP	24126761
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	TAP	24981860
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Anti Tag CoIP	26496610
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Y2H Array	32296183
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Pull Down	20811460
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	X-Ray Diffraction	11927569
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Y2H Prey Pooling	32296183
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	GMS	12527904
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Anti Bait CoIP	7760808
Intra	RPA1	P27694	RPA2	Homo sapiens	P15927	Chromatography	2159011
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Anti Bait CoIP	7760808
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	TAP	24981860
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Y2H	19338310
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	TAP	24126761
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Anti Tag CoIP	26496610
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	X-Ray Diffraction	11927569
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Chromatography	2159011
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	GMS	12527904
Intra	RPA1	P27694	RPA3	Homo sapiens	P35244	Pull Down	20811460
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59	Y2H Array	25416956
Intra	RPA1	P27694	MTUS2	Homo sapiens	Q5JR59	Y2H Prey Pooling	25416956
Intra	RPA1	P27694	POLA1	Homo sapiens	P09884	CoIP	17666013

Cross: Cross-species interaction Intra: Intraspecies interaction

RPA1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80884	RPA70 Antibody (YA678)	WB, ICC/IF, IP	Human, Monkey, Mouse, Rat
HY-P80884A	RPA70 Antibody (YA678)(PBS only)	WB, ICC/IF, IP	Human, Monkey, Mouse, Rat
HY-P85588	RPA70 Antibody (YA5280)	WB, ICC/IF, IP	Human, Monkey, Mouse, Rat

Related Diseases

Diseases	Alias
Pulmonary Fibrosis And/Or Bone Marrow Failure, Telomere-Related, 6	PFBMFT6 Pulmonary Fibrosis, And/Or Bone Marrow Failure, Telomere-Related, 6
Ataxia-Telangiectasia	Ataxia Telangiectasia Louis-Bar Syndrome AT At1 Ataxia-Telangiectasia Syndrome Ataxia - Telangiectasia Variant Boder-Sedgwick Syndrome Louis Bar Syndrome Cerebello-Oculocutaneous Telangiectasia Immunodeficiency With Ataxia Telangiectasia A-T Ataxia Telangiectasia Syndrome Atm Telangiectasia, Cerebello-Oculocutaneous Ataxia-Telangiectasia Variant
Werner Syndrome	Werner'S Syndrome WRN Adult Progeria Ws Adult Premature Ageing Syndrome Adult Premature Aging Syndrome Werners Syndrome
Xeroderma Pigmentosum, Variant Type	Xeroderma Pigmentosum XPV Xeroderma Pigmentosum Variant Type Xeroderma Pigmentosum With Normal Dna Repair Rates Photosensitivity With Defective Dna Synthesis Xp De Sanctis-Cacchione Syndrome Desanctis-Cacchione Syndrome Xeroderma Pigmentosa Xerodermic Idiocy Xeroderma Pigmentosum Variant Xp - [Xeroderma Pigmentosum] Atrophoderma Pigmentosum
Microphthalmia, Syndromic 10	MCOPS10 Moba Microphthalmia And Brain Atrophy Syndromic Microphthalmia 10 Microphthalmia-Brain Atrophy Syndrome Microphthalmia Syndromic 10 Moba Syndrome Syndromic Microphthalmia Type 10
Bloom Syndrome	BLM Bs Bls Bloom-Torre-Machacek Syndrome Microcephaly, Growth Restriction, And Increased Sister Chromatid Exchange 1 Mgrisce1 Congenital Telangiectatic Erythema Congenital Telangiectatic Erythema Syndrome Growth Deficiency, Sun-Sensitive, Telangiectatic, Hypo And Hyperpigmented Skin, Predisposition To Malignancy And Chromosomal Instability Bloom'S Syndrome Bsyn
Trichothiodystrophy	Ttd Amish Brittle Hair Syndrome Bids Syndrome Brittle Hair-Intellectual Impairment-Decreased Fertility-Short Stature Syndrome Ibids Pibids Trichothiodystrophy Syndromes
Alzheimer Disease 13	AD13 Alzheimer Disease-13 Alzheimer'S Disease 13 Alzheimer'S Disease 13, Late Onset
Nijmegen Breakage Syndrome	Berlin Breakage Syndrome NBS Microcephaly, Normal Intelligence And Immunodeficiency Ataxia-Telangiectasia Variant Ataxia-Telangiectasia Variant V1 Seemanova Syndrome Ii Immunodeficiency-Microcephaly-Chromosomal Instability Syndrome Seemanova Syndrome Type 2 At-V1 Microcephaly With Normal Intelligence, Immunodeficiency, And Lymphoreticular Malignancies Nonsyndromal Microcephaly, Autosomal Recessive, With Normal Intelligence Immunodeficiency, Microcephaly, And Chromosomal Instability Microcephaly-Immunodeficiency-Lymphoreticuloma Syndrome Microcephaly Immunodeficiency Lymphoreticuloma Microcephaly With Normal Intelligence Immunodeficiency And Lymphoreticular Malignancies Nonsyndromal Microcephaly Autosomal Recessive With Normal Intelligence Seemanova Syndrome 2 Ataxia-Telangiectasia Variant 1 Seemanova Syndrome At V1 Ataxia-Telangiectasia, Variant 1 Microcephaly-Immunodeficiency-Lymphoid Malignancy Syndrome V-At Ataxia Telangiectasia Variant V1
Haverhill Fever	Streptobacillosis Streptobacillary Rat-Bite Fever Streptobacillary Fever Rat-Bite Fever Due To Streptobacillus Moniliformis Erythema Arthriticum Epidemicum Epidemic Arthritic Erythema
Xeroderma Pigmentosum, Complementation Group A	Xeroderma Pigmentosum Group A Xp1 Xeroderma Pigmentosum, Group A XPA Xeroderma Pigmentosum I Xeroderma Pigmentosum Complementation Group A Xp Group A Xp, Group A Xeroderma Pigmentosum 1 Xeroderma Pigmentosum, Type 1 XP-A
Seckel Syndrome	Microcephalic Primordial Dwarfism Bird-Headed Dwarfism Harper'S Syndrome Virchow-Seckel Dwarfism Nanocephalic Dwarfism Sckl Seckel-Type Dwarfism
Fanconi Anemia, Complementation Group A	Fanconi Anemia Fanconi Pancytopenia Fanconi Anemia Complementation Group A FANCA Fa Fanconi Panmyelopathy Fanconi'S Anemia Fanconi Anaemia Fanconi'S Anaemia Fanconi Hypoplastic Anemia Estren-Dameshek Variant Of Fanconi Anemia Estren-Dameshek Variant Of Fanconi Pancytopenia Fanconi Anemia Estren-Dameshek Variant Fanconis Anemia

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (5)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-155655	JC-229	Inhibitor	97.07%	Unkown
HY-RS10837	POLR1A Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS12142	RPA1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS17297	Rpa1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS23752	Rpa1 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Rattus norvegicus	RPA1	RGD	RGD:1307376
Felis catus	RPA1	VGNC	VGNC:64726
Canis familiaris	RPA1	VGNC	VGNC:45702
Bos taurus	RPA1	VGNC	VGNC:34094
Mus musculus	RPA1	MGD	MGI:1915525
Others	RPA1	NCBI