RPA2 - replication protein A2 Gene

Also Known as REPA2; RPA32; RP-A p32; RP-A p34

Species: Homo sapiens

Gene Type: protein coding
Gene ID: 6118

About RPA2

Cytogenetic location: 1p35.3 Genomic coordinates (GRCh38): 1:27,891,524-27,914,797 (from NCBI)

This gene has 5 transcripts (splice variants), 244 orthologues and 2 paralogues. Ubiquitous expression in testis (RPKM 29.0), lymph node (RPKM 26.6) and 25 other tissues.

Summary

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]

RPA2 Products (5)

mRNA Protein Name
NM_001286076.2 NP_001273005.1 replication protein A 32 kDa subunit isoform 2
NM_001297558.1 NP_001284487.1 replication protein A 32 kDa subunit isoform 3
NM_001355128.2 NP_001342057.1 replication protein A 32 kDa subunit isoform 2
NM_001355129.2 NP_001342058.1 replication protein A 32 kDa subunit isoform 4
NM_002946.5 NP_002937.1 replication protein A 32 kDa subunit isoform 1

Gene Ontology

  • Molecular Function
  • Biological Process
  • Cellular Component
Molecular Function GO Annotation Evidence References Source
enables G-rich strand telomeric DNA binding IDA
IDA: Inferred from direct assay
24747047 GOA
enables damaged DNA binding IDA
IDA: Inferred from direct assay
7700386 GOA
enables enzyme binding IPI
IPI: Inferred from physical interaction
19793862 GOA
enables protein binding IPI
IPI: Inferred from physical interaction
2159011 GOA
enables protein phosphatase binding IPI
IPI: Inferred from physical interaction
20154705 GOA
enables single-stranded DNA binding IDA
IDA: Inferred from direct assay
19010961 GOA
enables ubiquitin protein ligase binding IPI
IPI: Inferred from physical interaction
21504906 GOA
Biological Process GO Annotation Evidence References Source
involved in DNA replication IDA
IDA: Inferred from direct assay
2406247 GOA
involved in DNA replication IMP
IMP: Inferred from mutant phenotype
9430682 GOA
involved in base-excision repair IDA
IDA: Inferred from direct assay
9765279 GOA
involved in double-strand break repair via homologous recombination IMP
IMP: Inferred from mutant phenotype
17765923 GOA
involved in mismatch repair IMP
IMP: Inferred from mutant phenotype
9430682 GOA
involved in mitotic G1 DNA damage checkpoint signaling IMP
IMP: Inferred from mutant phenotype
21731742 GOA
involved in nucleotide-excision repair IMP
IMP: Inferred from mutant phenotype
9430682 GOA
involved in protein localization to chromosome IDA
IDA: Inferred from direct assay
27723717 GOA
involved in regulation of DNA damage checkpoint IMP
IMP: Inferred from mutant phenotype
21504906 GOA
involved in regulation of double-strand break repair via homologous recombination IMP
IMP: Inferred from mutant phenotype
20154705 GOA
involved in telomere maintenance IMP
IMP: Inferred from mutant phenotype
17959650 GOA
Cellular Component GO Annotation Evidence References Source
part of DNA replication factor A complex IDA
IDA: Inferred from direct assay
2406247 GOA
part of DNA replication factor A complex IPI
IPI: Inferred from physical interaction
11927569 GOA
colocalizes with PML body IDA
IDA: Inferred from direct assay
17959650 GOA
located in PML body IDA
IDA: Inferred from direct assay
12814551 GOA
located in chromatin IDA
IDA: Inferred from direct assay
10336450 GOA
colocalizes with chromosome, telomeric region IDA
IDA: Inferred from direct assay
17959650 GOA
located in nucleus IDA
IDA: Inferred from direct assay
10982866 GOA
colocalizes with site of double-strand break IDA
IDA: Inferred from direct assay
21504906 GOA
EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RPA2 Protein Structure

tRNA_anti-codon

tRNA_anti-codon: OB-fold nucleic acid binding domain (74 - 148)

RPA_C

RPA_C: Replication protein A C terminal (166 - 262)

  • 0
  • 100
  • 200
  • 270 a.a.
Protein Preferred Names Protein Names

replication protein A 32 kDa subunit

  • RF-A protein 2

RPA2 Protein-protein interaction Information

Type
Protein Name Protein ID Interactor Interactor Species Interactor ID Detection Method References
Intra
RPA2 P15927 UNG Homo sapiens P13051
Y2H
10982866
Intra
RPA2 P15927 UNG Homo sapiens P13051
ITC
24910198
Intra
RPA2 P15927 RFWD3 Homo sapiens Q6PCD5
TAP
24126761
Intra
RPA2 P15927 TIPIN Homo sapiens Q9BVW5
TAP
24126761
Intra
RPA2 P15927 TIPIN Homo sapiens Q9BVW5
ITC
24910198
Intra
RPA2 P15927 XPA Homo sapiens P23025
Y2H
10982866
Intra
RPA2 P15927 XPA Homo sapiens P23025
Validated Y2H
32296183
Intra
RPA2 P15927 GAPDH Homo sapiens P04406
Y2H Pooling
16169070
Intra
RPA2 P15927 CDC45 Homo sapiens O75419
Pull Down
23910567
Intra
RPA2 P15927 CCNC Homo sapiens P24863
Validated Y2H
32296183
Intra
RPA2 P15927 SMARCAL1 Homo sapiens Q9NZC9
X-Ray Diffraction
24910198
Intra
RPA2 P15927 SMARCAL1 Homo sapiens Q9NZC9
GMS
24910198
Intra
RPA2 P15927 SMARCAL1 Homo sapiens Q9NZC9
TAP
24126761
Intra
RPA2 P15927 SMARCAL1 Homo sapiens Q9NZC9
ITC
24910198
Intra
RPA2 P15927 RPA1 Homo sapiens P27694
Y2H
15965237
Intra
RPA2 P15927 RPA1 Homo sapiens P27694
TAP
24126761
Intra
RPA2 P15927 RPA1 Homo sapiens P27694
Anti Tag CoIP
33961781
Intra
RPA2 P15927 RPA1 Homo sapiens P27694
Anti Tag CoIP
34591612
Intra
RPA2 P15927 RPA1 Homo sapiens P27694
Validated Y2H
32296183
Intra
RPA2 P15927 RPA1 Homo sapiens P27694
Anti Bait CoIP
24126761
Intra
RPA2 P15927 RPA3 Homo sapiens P35244
Anti Tag CoIP
34591612
Intra
RPA2 P15927 RPA3 Homo sapiens P35244
Y2H
19338310
Intra
RPA2 P15927 RPA3 Homo sapiens P35244
TAP
24981860
Intra
RPA2 P15927 RPA3 Homo sapiens P35244
TAP
24126761
Intra
RPA2 P15927 RPA3 Homo sapiens P35244
Anti Tag CoIP
33961781
Intra
RPA2 P15927 RPA3 Homo sapiens P35244
Y2H
10982866
Intra
RPA2 P15927 EIF4EBP3 Homo sapiens O60516
Anti Tag CoIP
22684010
Intra
RPA2 P15927 SERTAD3 Homo sapiens Q9UJW9
Y2H
10982866
Intra
RPA2 P15927 SERTAD3 Homo sapiens Q9UJW9
Pull Down
10982866
Cross: Cross-species interaction Intra: Intraspecies interaction

RPA2 Antibodies

Cat. No. Product Name Application Reactivity
HY-P80975 RPA32/RPA2 Antibody (YA679) WB, IHC-P, ICC/IF, IP Human, Mouse, Rat
HY-P80975A RPA32/RPA2 Antibody (YA679)(PBS only) WB, IHC-P, ICC/IF, IP Human, Mouse, Rat
HY-P82975 Phospho-RPA32/RPA2 (Thr21) Antibody (YA2720) WB, IP Human, Mouse, Rat
HY-P84119 RPA32/RPA2 Antibody (YA3816) WB, IHC-P, FC Human
HY-P84119A RPA32/RPA2 Antibody (YA3816)(PBS only) WB, IHC-P, FC Human
HY-P85591 RPA32/RPA2 Antibody (YA5283) WB, IP, ICC/IF Human
HY-P86488 RPA32/RPA2 Antibody (YA6180) WB, IHC-P, ICC/IF, IP, ELISA Human, Mouse, Rat

Related Diseases

Diseases Alias
Ataxia-Telangiectasia

  • Ataxia Telangiectasia

  • Louis-Bar Syndrome

  • AT

  • At1

  • Ataxia-Telangiectasia Syndrome

  • Ataxia - Telangiectasia Variant

  • Boder-Sedgwick Syndrome

  • Louis Bar Syndrome

  • Cerebello-Oculocutaneous Telangiectasia

  • Immunodeficiency With Ataxia Telangiectasia

  • A-T

  • Ataxia Telangiectasia Syndrome

  • Atm

  • Telangiectasia, Cerebello-Oculocutaneous

  • Ataxia-Telangiectasia Variant
Xeroderma Pigmentosum, Variant Type

  • Xeroderma Pigmentosum

  • XPV

  • Xeroderma Pigmentosum Variant Type

  • Xeroderma Pigmentosum With Normal Dna Repair Rates

  • Photosensitivity With Defective Dna Synthesis

  • Xp

  • De Sanctis-Cacchione Syndrome

  • Desanctis-Cacchione Syndrome

  • Xeroderma Pigmentosa

  • Xerodermic Idiocy

  • Xeroderma Pigmentosum Variant

  • Xp - [Xeroderma Pigmentosum]

  • Atrophoderma Pigmentosum
Bloom Syndrome

  • BLM

  • Bs

  • Bls

  • Bloom-Torre-Machacek Syndrome

  • Microcephaly, Growth Restriction, And Increased Sister Chromatid Exchange 1

  • Mgrisce1

  • Congenital Telangiectatic Erythema

  • Congenital Telangiectatic Erythema Syndrome

  • Growth Deficiency, Sun-Sensitive, Telangiectatic, Hypo And Hyperpigmented Skin, Predisposition To Malignancy And Chromosomal Instability

  • Bloom'S Syndrome

  • Bsyn
Xeroderma Pigmentosum, Complementation Group A

  • Xeroderma Pigmentosum Group A

  • Xp1

  • Xeroderma Pigmentosum, Group A

  • XPA

  • Xeroderma Pigmentosum I

  • Xeroderma Pigmentosum Complementation Group A

  • Xp Group A

  • Xp, Group A

  • Xeroderma Pigmentosum 1

  • Xeroderma Pigmentosum, Type 1

  • XP-A
Xeroderma Pigmentosum, Complementation Group C

  • Xeroderma Pigmentosum, Group C

  • XPC

  • Xpcc

  • Xeroderma Pigmentosum Iii

  • Xp3

  • Xeroderma Pigmentosum Group C

  • Xp Group C

  • Xp, Group C

  • Xeroderma Pigmentosum, Type 3

  • Xeroderma Pigmentosum Complementation Group C

  • XP-C
Seckel Syndrome

  • Microcephalic Primordial Dwarfism

  • Bird-Headed Dwarfism

  • Harper'S Syndrome

  • Virchow-Seckel Dwarfism

  • Nanocephalic Dwarfism

  • Sckl

  • Seckel-Type Dwarfism
Fanconi Anemia, Complementation Group A

  • Fanconi Anemia

  • Fanconi Pancytopenia

  • Fanconi Anemia Complementation Group A

  • FANCA

  • Fa

  • Fanconi Panmyelopathy

  • Fanconi'S Anemia

  • Fanconi Anaemia

  • Fanconi'S Anaemia

  • Fanconi Hypoplastic Anemia

  • Estren-Dameshek Variant Of Fanconi Anemia

  • Estren-Dameshek Variant Of Fanconi Pancytopenia

  • Fanconi Anemia Estren-Dameshek Variant

  • Fanconis Anemia
Diseases Alias
Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Macroglobulinemia Of Waldenstrom

Lymphoplasmacytic Lymphoma With Igm Gammopathy

Lymphoplasmacytic Lymphoma

Waldenstroem'S Macroglobulinemia

Waldenstroem'S Macroglobulinemia

Macroglobulinemia Of Waldenstrom

Lymphoplasmacytic Lymphoma With Igm Gammopathy

Lymphoplasmacytic Lymphoma

Related Agents

  • Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (5)
Cat. No. Product Name
Effect
Purity
Rare Diseases
HY-RS10838 POLR1B Human Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-RS12143 RPA2 Human Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-RS17591 Rpa2 Mouse Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-RS21910 Polr1b Mouse Pre-designed siRNA Set A Pre-designed Set / Unkown
HY-RS24051 Rpa2 Rat Pre-designed siRNA Set A Pre-designed Set / Unkown

Orthologs Information

Species Symbol Source ID
Mus musculus RPA2 MGD MGI:1339939
Bos taurus RPA2 VGNC VGNC:34095
Felis catus RPA2 VGNC VGNC:80345
Macaca mulatta RPA2 VGNC VGNC:100064
Rattus norvegicus RPA2 RGD RGD:619714
Canis familiaris RPA2 VGNC VGNC:45703
Others RPA2 NCBI