2. Immunology/Inflammation
  3. NO Synthase
  4. iNOs-IN-9

iNOs-IN-9 is a selective inducible nitric oxide synthase (iNOS) inhibitor with an IC50 of 82 nM against hiNOS. iNOs-IN-9 reduces cytokine-induced inflammatory responses and cell necrosis in inflammatory cell models. iNOs-IN-9 can be used for research related to psoriasis.

For research use only. We do not sell to patients.

iNOs-IN-9

iNOs-IN-9 Chemical Structure

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iNOs-IN-9 Related Antibodies

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Description

iNOs-IN-9 is a selective inducible nitric oxide synthase (iNOS) inhibitor with an IC50 of 82 nM against hiNOS. iNOs-IN-9 reduces cytokine-induced inflammatory responses and cell necrosis in inflammatory cell models. iNOs-IN-9 can be used for research related to psoriasis[1].

IC50 & Target[1]

iNOS

82 nM (IC50)

In Vitro

iNOs-IN-9 (Compound 10) (0.01-10 μM; 15 min preincubation, 30 min incubation at 37 °C) potently and selectively inhibits human iNOS with an IC50 of 82 nM, showing no significant inhibition of bovine eNOS at concentrations up to 10 μM and a selectivity ratio >116[1].
iNOs-IN-9 (10 μM; duration of cytokine stimulation) downregulates cytokine-induced inflammatory gene and membrane marker expression in primary human keratinocytes, with strong inhibitory effects on CXCL8, CCL2, CXCL10, ICAM-1, HLA-DR, and MHC class I[1].
iNOs-IN-9 (20 μM; 3 h) preserves histological integrity of healthy human skin explants and significantly reduces cytokine-induced inflammatory gene expression in this ex vivo model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

iNOs-IN-9 Related Antibodies

Cell Viability Assay[1]

Cell Line: HaCaT human keratinocytes (basal and pro-inflammatory conditions stimulated with TNF-α, IFN-γ, and IL-17)
Concentration: 0.1-100 μM
Incubation Time: 24 h, 48 h
Result: Did not alter HaCaT cell metabolic activity at any tested concentration after 24 or 48 h under basal conditions.
Slightly but significantly restored cell metabolic activity at concentrations of 50 and 100 μM after 24 h, and at concentrations of 20, 50, and 100 μM after 48 h under pro-inflammatory conditions, compared to cytokine-stimulated controls.
Decreased nitric oxide release in cytokine-stimulated HaCaT cells after 24 h.
In Vivo

iNOs-IN-9 (1-30 μM; continuous immersion) exhibits good tolerance when applied at concentrations of 10 μM and 20 μM to zebrafish larvae within the 48-120 hpf window, but shows significant toxicity when administered at concentrations of 10 μM and above to early embryos within the 3-96 hpf window[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: AB wild-type[1]
Dosage: 1 μM, 10 μM, 20 μM, 30 μM
Administration: continuous immersion; duration dependent on exposure window (3-96 hpf or 48-120 hpf)
Result: Resulted in 100% survival with very low malformation rates not statistically different from controls when administered at 10 μM and 20 μM during 48-120 hpf exposure window.
Caused significant mortality and sublethal effects when administered at 10 μM during 3-96 hpf exposure window.
Resulted in 100% mortality and severe malformations including pericardial edema, scoliosis, craniofacial defects, and yolk deformation when administered at 20 μM and 30 μM during 3-96 hpf exposure window.
Molecular Weight

323.31

Formula

C16H13N5O3
SMILES

O=C(NC1=CC2=C(N=C1)C=CC=C2)CNC3=NC=CC=C3[N+]([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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iNOs-IN-9 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

iNOs-IN-9NO SynthaseNitric oxide synthasesNOShuman monocytespsoriasismacrophagesinducible nitric oxide synthasehuman skin explantsprimary human keratinocytesrat liver microsomesHaCaT human keratinocyteszebrafish larvaenitric oxideInhibitorinhibitorinhibit

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