2. Others Apoptosis Metabolic Enzyme/Protease Immunology/Inflammation NF-κB
  3. Fluorescent Dye Apoptosis Reactive Oxygen Species (ROS)
  4. IR-780

IR-780 is a near-infrared fluorescent probe for in vivo imaging of tumor cells. IR-780 is transported into tumor cells via OATPs and ABCB10, with uptake dependent on glycolytic activity and plasma membrane potential. IR-780 preferentially accumulates in tumor cell mitochondria, including those of drug-resistant cancer cells, without chemical conjugation. IR-780 generates reactive oxygen species (ROS), induces hyperthermia and apoptosis, inhibits tumor growth and recurrence, and modulates HSP70 expression upon ultrasound or 808 nm laser exposure. IR-780 acts as a sonosensitizer, photodynamic and photothermal agent, and drug delivery carrier, with low acute imaging-dose toxicity and rapid vital organ clearance. IR-780 can be used for the research of cancer, such as breast cancer, lung cancer, and non-small cell lung cancer (NSCLC).

For research use only. We do not sell to patients.

CAS No. : 207399-07-3

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Customer Review

Based on 6 publication(s) in Google Scholar

IR-780 Related Antibodies

Top Publications Citing Use of Products

    IR-780 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 15;16(1):6532.  [Abstract]

    Representative fluorescence image of subcutaneous 4T1 tumor-bearing mice at different time after intravenous injection with IR-780 (IR780; 0.5 mg/mL; 37 °C for 15 min; IV; 100 μL)-labeled SRB, FeS@BSA and FeS@SRB, respectively.

    IR-780 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 15;16(1):6532.  [Abstract]

    Representative fluorescence images of orthotopic 4T1 tumor-bearing mice at different time after intravenous injection with IR-780 (IR780; 0.5 mg/mL; 37 °C for 15 min; IV; 100 μL)-labeled SRB, FeS@BSA and FeS@SRB, respectively, and the corresponding fluorescence images of tumors and organs at 48 h.

    IR-780 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Dec 31.  [Abstract]

    MTT cytotoxicity profiles of IR-780 (0-2.5 μM), IR780@BSA, and IR780@HS at varying concentrations (48 h treatment) in HepG2, MCF-7, and 4T1 cell lines.

    IR-780 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Dec 31.  [Abstract]

    Viability assessment via Calcein-AM/PI dual staining in HepG2 cells following a 24 h treatment with IR780 ( 0.25 μM)@HS.

    IR-780 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Dec 31.  [Abstract]

    Wound-healing and migration assays indicated that IR780 ( 0.25 μM)@HS markedly suppressed HepG2 cell migration relative to the control groups.

    IR-780 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Dec 31.  [Abstract]

    Cellular internalization mechanisms of IR780@HS. (A−C) Confocal microscopy images.

    • Biological Activity

    • Purity & Documentation

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    • Customer Review

    Description

    IR-780 is a near-infrared fluorescent probe for in vivo imaging of tumor cells. IR-780 is transported into tumor cells via OATPs and ABCB10, with uptake dependent on glycolytic activity and plasma membrane potential. IR-780 preferentially accumulates in tumor cell mitochondria, including those of drug-resistant cancer cells, without chemical conjugation. IR-780 generates reactive oxygen species (ROS), induces hyperthermia and apoptosis, inhibits tumor growth and recurrence, and modulates HSP70 expression upon ultrasound or 808 nm laser exposure. IR-780 acts as a sonosensitizer, photodynamic and photothermal agent, and drug delivery carrier, with low acute imaging-dose toxicity and rapid vital organ clearance. IR-780 can be used for the research of cancer, such as breast cancer, lung cancer, and non-small cell lung cancer (NSCLC)[1][2][3][4][5][6][7][8][9][11].

    In Vitro

    IR-780 (10 μM; 15 min at 37°C) preferentially accumulates in the mitochondria of human MCF-7, HeLa, and MG-63 tumor cells via organic anion transporter peptide-mediated uptake[1].
    IR-780 acts as an SDT agent in 4T1 breast cancer cells, generating increased ROS upon US irradiation[2].
    IR-780 selectively accumulates in the mitochondria of A549/DR drug-resistant lung cancer cells, inhibiting cell growth and promoting apoptosis by disrupting mitochondrial function[2].
    IR-780 (4-16 μM; 1-3 h (flow cytometry); 1 h (confocal microscopy)) exhibited dose- and time-dependent uptake by 4T1 breast cancer cells, with higher concentrations and longer incubation times leading to greater uptake[3].
    IR-780 (10 μM; 3 h (pre-incubation); 20 s US irradiation)-induced sonodynamic cell damage in 4T1 breast cancer cells is inhibited by histidine and SOD but not mannitol[3].
    IR-780 (2.5-40 μM; 48-72 h) inhibits the viability of human cancer cell lines (A549, H460, HepG2, U251, MCF-7) with A549 cells being the most susceptible[4].
    IR-780 (10 μM; 4 h) significantly inhibits the clone formation ability of A549/DR cells[4].
    IR-780 (10 μM; 12 h) significantly inhibits the migration ability of A549/DR cells[4].
    IR-780 (20 μM; 20 min) increases ROS production in A549/DR cells[4].
    IR-780 (10-20 μM; 24 h) decreases the mitochondrial membrane potential of A549/DR cells, with significant reduction at 20 μM[4].
    IR-780 (20 μM; 24 h) induces apoptosis in over 60% of A549/DR cells after 24 h[4].
    IR-780 (4-16 μM; 20-40 s ultrasound irradiation) acted as a sonosensitizer to significantly reduce 4T1 murine breast cancer cell viability and induce apoptosis/necrosis in a dose- and ultrasound time-dependent manner, with higher concentrations and longer ultrasound exposure causing greater cytotoxicity[9].
    IR-780 (5 μg/mL equivalent free IR-780; 10-120 min) loaded into heparin-folic acid nanoparticles exhibits time-dependent fluorescence activation in MCF-7 cells, with fluorescence intensity increasing significantly over 120 min of incubation, indicating cellular uptake and release of active IR-780[11].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    IR-780 Related Antibodies

    Cell Viability Assay[4]

    Cell Line: A549, H460, HepG2, U251, MCF-7
    Concentration: 0, 2.5, 5, 10, 20, 40 μM
    Incubation Time: 48 h, 72 h
    Result: Displayed anticancer activity across all tested cell lines; identified A549 cells as the most susceptible. Calculated IC50 values for each cell line at 48 h and 72 h.

    Cell Viability Assay[4]

    Cell Line: A549, A549/DR
    Concentration: 0, 2.5, 5, 10, 15, 20 μM
    Incubation Time: 48 h
    Result: Showed concentration-dependent cytotoxicity on A549 cells, eliminating nearly all cells at 20 μM. Exhibited similar cytotoxicity to both A549 and A549/DR cells across concentrations, and effectively suppressed A549/DR cells, nearly killing all at 20 μM.

    Apoptosis Analysis[4]

    Cell Line: A549/DR
    Concentration: 0, 10, 20 μM
    Incubation Time: 24 h
    Result: Induced apoptosis in more than 60% of A549/DR cells after incubation with 20 μM IR-780 for 24 h, compared to 7% in the control group.
    In Vivo

    IR-780 iodide (0.2 mg/kg; i.v.; single dose) preferentially accumulates in multiple human tumor xenografts in nude mice, maintaining a detectable fluorescence signal with an average contrast index of 7 for at least 20 days post-injection[1].
    IR-780 iodide (0.2 mg/kg; i.v.; single dose) preferentially accumulates in chemically induced lung tumors in immune-intact C57BL/6 mice, enabling clear tumor visualization via near-infrared fluorescence imaging[1].
    IR-780 (80 μg; intratumoral injection; followed by US irradiation at 2 W/cm2 for 4 minutes) as a sonosensitizer combined with US significantly inhibits breast tumor growth and increases tumor cell apoptosis in BALB/c mice[3].
    IR-780 (5.0 mg/kg; i.p.; every two days; 4-5 times) exhibits potent tumoricidal activity and inhibits tumor recurrence in a mouse LLC xenograft model, with a tumor formation rate of 40% in secondary mice[4].
    IR-780 (5.74 μmol/kg; i.v.; every three days; 15 days; 808 nm laser irradiation) exerts tumor growth inhibition in Hep1-6 tumor-bearing BALB/c mice[6].
    IR-780 (80 μg; intratumoral injection; followed by ultrasound irradiation at 2 W/cm2 for 4 minutes) combined with US irradiation induces significant breast tumor growth inhibition in BALB/c mice, reducing mean tumor volume to 40.7 mm3 at day 30 and achieving a 50.9 % tumor cell apoptotic index, with no significant body weight changes[9].
    IR-780 (0.7 mg/kg; i.v.; single dose) accumulates in MCF-7 breast cancer xenografts, enabling NIR fluorescence imaging, though with lower tumor retention than IR-780 delivered via HF-IR-780 NPs[11].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Athymic nude mice (5-6 weeks old, 20-25 g)[1]
    Dosage: 0.2 mg/kg
    Administration: i.v.; single dose
    Result: Visualized intense, specific near-infrared fluorescence signals from MCF-7, HeLa, and MG-63 tumor xenografts with low background interference at 48 hours post-injection. Detected fluorescence signal persisting in tumors for at least 20 days, with contrast index (CI) values ranging from 4 to 14 and an average CI of 7 on day 20.
    Animal Model: BALB/c (female, 6 to 10 week old) injected with 4T1 breast carcinoma cells.[3]
    Dosage: 80 μg
    Administration: intratumoral injection; followed by US irradiation at 2 W/cm² for 4 minutes
    Result: Achieved mean tumor size of 40.7 mm3 at day 30; showed significant tumor growth inhibition; induced 50.9% apoptotic tumor cells (TUNEL-positive); caused significantly increased degree of cell necrosis.
    Animal Model: C57 BL/6 (male, 8 weeks old, 20-25 g)[4]
    Dosage: 5.0 mg/kg
    Administration: i.p.; every two days; 5 times; 4 times
    Result: Significantly suppressed tumor growth compared to control group and ADM group.
    Reduced tumor weight compared to control, CTX, and ADM groups.
    Produced much smaller tumors than ADM group at day 16.
    Induced a 40% tumor formation rate in secondary mice from treated xenograft cells.
    Animal Model: BALB/c (male, 6-8 weeks, 18-22 g, Hep1-6 tumor model)[6]
    Dosage: 5.74 μmol/kg
    Administration: i.v.; every three days; 15 days; 808 nm NIR laser (1 W/cm²) irradiation for 5 min at 6 h post-injection
    Result: Exerted tumor growth inhibition (a small number of trypan blue-stained cells observed in in vitro correlate studies), though less potent than the IR780-LA/CPT-ss-CPT NPs + laser group
    Animal Model: BALB/c (female, 6-8 weeks old; 4T1 breast carcinoma xenograft)[9]
    Dosage: 80 μg
    Administration: intratumoral injection; followed by ultrasound irradiation at 2 W/cm² for 4 minutes
    Result: Achieved mean tumor volume of 40.7 mm3 at day 30. Induced 50.9% apoptotic tumor cells via TUNEL staining. Observed significant tumor cell necrosis via histological analysis. Detected no significant changes in mouse body weight during the study.
    Molecular Weight

    667.11

    Formula

    C36H44ClIN2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to green yellow

    SMILES

    CCC[N+]1=C(/C=C/C2=C(Cl)/C(CCC2)=C/C=C3N(CCC)C4=C(C=CC=C4)C\3(C)C)C(C)(C)C5=C1C=CC=C5.[I-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 12.5 mg/mL (18.74 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4990 mL 7.4950 mL 14.9900 mL
    5 mM 0.2998 mL 1.4990 mL 2.9980 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    In Vivo Dissolution Calculator
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    Purity & Documentation

    Purity: 99.82%

    SDS (394 KB)

    COA (253 KB) HNMR (281 KB) RP-HPLC (249 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.4990 mL 7.4950 mL 14.9900 mL 37.4751 mL
    5 mM 0.2998 mL 1.4990 mL 2.9980 mL 7.4950 mL
    10 mM 0.1499 mL 0.7495 mL 1.4990 mL 3.7475 mL
    15 mM 0.0999 mL 0.4997 mL 0.9993 mL 2.4983 mL
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    IR-780 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    IR-780207399-07-3IR780IR 780Fluorescent DyeApoptosisReactive Oxygen Species (ROS)MG-63OATPsABCB10apoptosisheat shock protein 70A549HeLareactive oxygen speciesMCF-7mitochondriaInhibitorinhibitorinhibit

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