2. Autophagy
  3. Autophagy
  4. KAN0438757

KAN0438757 is a potent and selective inhibitor of the metabolic kinase PFKFB3 with an IC50 of 0.19 μM.

For research use only. We do not sell to patients.

KAN0438757 Chemical Structure

KAN0438757 Chemical Structure

CAS No. : 1451255-59-6

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 302 In-stock
Solution
10 mM * 1 mL in DMSO USD 302 In-stock
Solid
1 mg USD 130 In-stock
5 mg USD 275 In-stock
10 mg USD 440 In-stock
50 mg USD 1089 In-stock
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 3 publication(s) in Google Scholar

KAN0438757 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

KAN0438757 is a potent and selective inhibitor of the metabolic kinase PFKFB3 with an IC50 of 0.19 μM[1].

IC50 & Target

IC50: 0.19 μM (PFKFB3)[1]
In Vitro

KAN0438757 (72 h) inhibits cell viability of Miapaca-2, PANC1, SW620, U-266, AMO-1 (IC50: 2.75, 3.83, 7.50, 5.08, 11.53 μM respectively)[1].
KAN0438757 (10 μM, 6 h) decreases homologous recombination (HR) activity and increases ionizing radiation (IR) induced γH2AX foci level in U2OS cells[1].
KAN0438757 (50 μM, 12 h) reduces PFKFB3 protein expression in HCT-116, SW-1463 and HUVECs[2].
KAN0438757 (0-50 μM, 24 h) reduces glycolysis in HCT-116 cancer cells and HUVECs[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

KAN0438757 Related Antibodies
In Vivo

KAN0438757 (i.p., 10; 25; 50 mg/kg) is well tolerated, and shows no relevant systemic toxic effects in C57BL6/N mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

447.43

Formula

C21H18FNO7S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OCCO)C1=CC=C(NS(=O)(C2=CC(C3=CC(F)=CC=C3O)=CC=C2)=O)C=C1O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 130 mg/mL (290.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2350 mL 11.1749 mL 22.3499 mL
5 mM 0.4470 mL 2.2350 mL 4.4700 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.17 mg/mL (4.85 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.17 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.17 mg/mL (4.85 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2350 mL 11.1749 mL 22.3499 mL 55.8747 mL
5 mM 0.4470 mL 2.2350 mL 4.4700 mL 11.1749 mL
10 mM 0.2235 mL 1.1175 mL 2.2350 mL 5.5875 mL
15 mM 0.1490 mL 0.7450 mL 1.4900 mL 3.7250 mL
20 mM 0.1117 mL 0.5587 mL 1.1175 mL 2.7937 mL
25 mM 0.0894 mL 0.4470 mL 0.8940 mL 2.2350 mL
30 mM 0.0745 mL 0.3725 mL 0.7450 mL 1.8625 mL
40 mM 0.0559 mL 0.2794 mL 0.5587 mL 1.3969 mL
50 mM 0.0447 mL 0.2235 mL 0.4470 mL 1.1175 mL
60 mM 0.0372 mL 0.1862 mL 0.3725 mL 0.9312 mL
80 mM 0.0279 mL 0.1397 mL 0.2794 mL 0.6984 mL
100 mM 0.0223 mL 0.1117 mL 0.2235 mL 0.5587 mL
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KAN0438757 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KAN04387571451255-59-6KAN 0438757KAN-0438757AutophagyInhibitorinhibitorinhibit

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