Saponins

Saponins purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Dec 1;17(1):253. [Abstract]

Customer Validation
Glutamate (20 μM) exposure significantly increased neuronal mitochondrial Ca²⁺ levels (shown by Rhod-2 fluorescence, representative graph scale bar: 20 μm). Detection: Neurons cultured 12–14 DIV were loaded with 2 μM Rhod-2-AM + 0.02% Pluronic F-127 (dark, RT, 30 min), washed, then incubated at 37°C for 30 min. Cells were permeabilized with 0.005% Saponins (1 min), rinsed with Ca²⁺-free HBSS, and finally bathed in extracellular buffer.

Saponins purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2021 Nov 29;12(12):1115. [Abstract]

Customer Validation
The mitochondrial calcium uptake assay in primary myoblasts from mice gastrocnemius muscle. The fluorescent dye Rhod-2/AM and Mito-Tracker Green were used to measure primary myoblasts mitochondrial Ca²⁺ uptake, Briefly, primary myoblasts were loaded with Rhod-2/AM and Mito-Tracker Green following the protocol, permeabilized with 0.005% Saponins for ~1 min, then incubated in Ca²⁺-free HBSS. Images were acquired at 2 frames/s using a spinning-disk confocal super-resolution microscope (excitation 549 nm, emission 578 nm). After 15 s, the buffer was switched to 5 μmol/L CaCl₂ solution, and imaging continued for an additional 50 s.

Saponins purchased from MedChemExpress. Usage Cited in: J Med Entomol. 2021 May 15;58(3):1202-1209. [Abstract]

Customer Validation
Transduction of P2C-EGFP-Cas9 RNP complex into Culex pipiens pallens ovaries. From left to right: P2C-EGFP-Cas9 + sgRNAs + chloroquine (n = 9); P2C-EGFP-Cas9 + sgRNAs + Saponins (n = 13); Wild-type (WT; n = 4). Scale bar: 1 mm. RNP, ribonucleoprotein; sgRNAs, single-guide RNAs.

Saponins purchased from MedChemExpress. Usage Cited in: J Med Entomol. 2021 May 15;58(3):1202-1209. [Abstract]

Customer Validation
Delivery efficiency of P2C-enhanced green fluorescent protein (EGFP)-Cas9 ribonucleoprotein with single-guide RNA and ERR.
Chl., Chloroquine; Sa., Saponins (50 mg/L; 1 μL); ERR, endosomal release reagent; PBM, post-bloodmeal
^#Injection mixture (Inj. Mix) = P2C-Cas9+sgRNAs+ERR (Chloroquine or Saponin).
^asgRNA935; ^bsgRNA936; ^csgRNA938.